Vajad kellegagi rääkida?
Küsi julgelt abi LasteAbi
Logi sisse

BIOinformaatika kodutöö 5 (0)

5 VÄGA HEA
Punktid

Bioinformaatika 5

Andmebaasid (KEGG, NCBI, RCSB), järjestuste (SMS, BioEdit) ja struktuuride (SPdbV) analüüs


  • KEGG-i kasutamine ( http://www.genome.jp/
  • Leida liikide Escherichia coli K12 MG1655, Drosophila melanogaster ja Homo sapiens glükolüüsi rajad ning ensüümid aldolaas ja püruvaatkinaas.
    Escherichia coli glükolüüsi rada:
    http://www.kegg.com/dbget-bin/get_pathway?org_name=eco&mapno=00010
    Drosophila glükolüüsi rada:
    http://www.kegg.com/dbget-bin/get_pathway?org_name=dme&mapno=00010
    Homo sapiens glükolüüsi rada:
    http://www.kegg.com/dbget-bin/get_pathway?org_name=hsa&mapno=00010
    aldodaas:
    http://www.kegg.com/dbget-bin/www_bget?enzyme+4.1.2.13
    püruvaatkinees:
    http://www.kegg.com/dbget-bin/www_bget?enzyme+2.7.1.40
  • Leida vastavad geenid – KEGG-i entry numbrid , geeni nimed, nukleotiidsed järjestused ning asukohad geenikaardil.
    Aldolaas :
    Escherichia coli K12 MG1655:
    http://www.kegg.com/dbget-bin/www_bget?eco+b2097
    Drosophila melanogaster:
    http://www.kegg.com/dbget-bin/www_bget?dme+Dmel_CG6058
    Homo sapiens:
    http://www.kegg.com/dbget-bin/www_bget?hsa+226
    Püruvaatkinaas :
    Escherichia coli K12 MG1655:
    http://www.kegg.com/dbget-bin/www_bget?eco+b1676
    Drosophila melanogaster:
    http://www.kegg.com/dbget-bin/www_bget?dme+Dmel_CG7069
    Homo sapiens:
    http://www.kegg.com/dbget-bin/www_bget?hsa+5313
  • Leida, milliste teiste metaboolsete radadega antud ensüümid on seotud.
    Aldolaas:
    Glycolysis / Gluconeogenesis
    http://www.kegg.com/dbget-bin/show_pathway?map00010+4.1.2.13
    Pentose phosphate pathway
    http://www.kegg.com/dbget-bin/show_pathway?map00030+4.1.2.13
    Inositol metabolism
    http://www.kegg.com/dbget-bin/show_pathway?map00031+4.1.2.13
    Fructose and mannose metabolism
    http://www.kegg.com/dbget-bin/show_pathway?map00051+4.1.2.13
    Carbon fixation
    http://www.kegg.com/dbget-bin/show_pathway?map00710+4.1.2.13
    Püruvaatkinaas:
    Glycolysis / Gluconeogenesis
    http://www.kegg.com/dbget-bin/show_pathway?map00010+4.1.2.13
    Purine metabolism
    http://www.kegg.com/dbget-bin/show_pathway?map00230+2.7.1.40
    Pyruvate metabolism
    http://www.kegg.com/dbget-bin/show_pathway?map00620+2.7.1.40
    Carbon fixation
    http://www.kegg.com/dbget-bin/show_pathway?map00710+2.7.1.40
    Insulin signaling pathway
    http://www.kegg.com/dbget-bin/show_pathway?map04910+2.7.1.40
    Type II diabetes mellitus
    http://www.kegg.com/dbget-bin/show_pathway?map04930+2.7.1.40
  • Leida vastavate geenidega seotud ensüümide klassid , aminohappelised järjestused, EC number.
    Aldolaas:
    EC 4.1.2.13   
    Class
    Lyases
    Carbon-carbon lyases
    Aldehyde-lyases
    Escherichia coli K12 MG1655:
    >eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (A)
    MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNNRPPAVLRNMQTLYNTG
    RLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAIEAGCNCVASTYGVLASVS
    RRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMGAVAVGATIYFGSEESRRQIE
    EISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSADLTGQANHLAATIGADIVKQKM
    AENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQLANCYMGRAGLINSGGAAGGETDLS
    DAVRTAVINKRAGGMGLILGRKAFKKSMADGVKLINAVQDVYLDSKITIA
    Drosophila melanogaster:
    >dme:Dmel_CG6058 Ald; Aldolase [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (A)
    MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRLQDIGVENTEDNRRAYR
    QLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKGIILGIKVDKGVVPLFGSE
    DEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPSYQSILENANVLARYASICQS
    QRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALSDHHVYLEGTLLKPNMVTAGQSA
    KKNTPEEIALATVQALRRTVPAAVTGVTFLSGGQSEEEATVNLSAINNVPLIRPWALTFS
    YGRALQASVLRAWAGKKENIAAGQNELLKRAKANGEAACGNYTAGSVKGFAGKDTLHVDD
    HRY
    Homo sapiens:
    >hsa:226 ALDOA; aldolase A, fructose-bisphosphate [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (A)
    MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYR
    QLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTN
    GETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQ
    NGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHAC
    TQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTF
    SYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVS
    NHAY
    Püruvaatkinaas:
    EC 2.7.1.40 
    Class
    Transferases
    Transferring phosphorus -containing groups
    Phosphotransferases with an alcohol group as acceptor
    Escherichia coli K12 MG1655:
    >eco:b1676 pykF; pyruvate kinase [EC:2.7.1.40]; K00873 pyruvate kinase (A)
    MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKT
    AAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVG
    NTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIF
    GCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGI
    MVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
    AILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDNRKLRITEAVCR
    GAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQLV
    KEITSTDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL
    Drosophila melanogaster:
    >dme:Dmel_CG7069 CG7069 [EC:2.7.1.40]; K00873 pyruvate kinase (A)
    MRVVRMNFSHGSHEYHCQTIQAARKAIAMYVEQTGLPRTLAIALDTKGPEIRTGKLAGGN
    DRAEIELKTGDKVTLSTKKEMADKSNKDNIYVDYQRLPQLVKPGNRVFVDDGLIALIVKE
    SKGDEVICQVENGGKLGSHKGINLPGVPVDLPSVTEKDKQDLKFGAEQKVDMIFASFIRD
    ANALKEIRQVLGPAGACIKIISKIENHQGLVNIDDIIRESDGIMVARGDMGIEIPTEDVP
    LAQKSIVAKCNKVGKPVICATQMMESMTNKPRPTRAEASDVANAIFDGCDAVMLSGETAK
    GKYPVECVQCMARICAKVEAVLWYESLQNSLKREIRTSAADHISAVTTAIAEAATVGQAR
    AIVVASPCSMVAQMVSHMRPPCPIVMLTGNESEAAQSLLFRGIYPLLVEEMVIGSFNFRR
    IMQSGLKLMGKMDILEPGQKGSVVLVNAMSAEKITFRLFTIRQQTKEERDQDERCRKLAL
    EQSCKERAEKEECRKLQQAEECQKQKLAKKCKQFEEKQKVCPKKNDTPKNDCPKKDCPKK
    ECPKQDDEISKCRQMQEAEAEERKCKEEFEQMCKLAEEKRKEAEKCRKADEERRKEEAEK
    CRKLEEDRKCKLAEEKKRNEEELKIIEAEVAKLEAAEKAKRLKEEEKKKEELMKCKQRNE
    AKKKREEAERCKRKERERELAEMENKWKQVAEKRKRKKAAEMCRKIEDAKEKAAAESADK
    ILKAVCEKLKQSLSDPDKSKKGKK
    Homo sapiens:
    >hsa:5313 PKLR; pyruvate kinase, liver and RBC [EC:2.7.1.40]; K00873 pyruvate kinase (A)
    MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ
    LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS
    HGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKG
    SQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQV
    ENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAA
    LGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRC
    NLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKM
    QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS
    AQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESG
    KLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
  • Kirjeldada nende ensüümide poolt katalüüsitavaid reaktsioone ja nendes osalevaid molekule.
    Aldolaas:
    http://www.kegg.com/dbget-bin/www_bget?rn+R01068
    Püruvaatkinaas:
    http://www.kegg.com/dbget-bin/www_bget?rn+R00200
  • Leida antud geenide/ensüümidega seotud haiguste kirjeldused (MIM) ja kirjanduse viited bibliograafilistest andmebaasidest.
    Aldolaas:
    Disease
    Aldolase A, fructose-bisphosphatase
    http://www.kegg.com/dbget-bin/www_bget?omim+103850
    Aldolase C, fructose-bisphosphatase
    http://www.kegg.com/dbget-bin/www_bget?omim+103870
    Aldolase B, fructose-bisphosphatase
    http://www.kegg.com/dbget-bin/www_bget?omim+229600
    Reference
    1
    Horecker, B.L., Tsolas, O. and Lai, C.Y. Aldolases, in Boyer, P.D.
    (Ed.), The Enzymes, 3rd edn., vol. 7, Academic Press, New York ,
    1972, pp. 213-258.
    2   http://www.kegg.com/dbget-bin/www_bget?medline+2649077
    Alefounder PR, Baldwin SA, Perham RN, Short NJ.
    Cloning, sequence analysis and over-expression of the gene for the
    class II fructose 1,6-bisphosphate aldolase of Escherichia coli.
    Biochem. J. 257 (1989) 529-34.
    Püruvaatkinaas:
    Disease
    Pyruvate kinase-3
    http://www.kegg.com/dbget-bin/www_bget?omim+179050
    Pyruvate kinase, liver and RBC type
    http://www.kegg.com/dbget-bin/www_bget?omim+609712
    Reference
    1
    Boyer, P.D. Pyruvate kinase. In: Boyer, P.D., Lardy, H. and Myrback,
    K. (Eds.), The Enzymes, 2nd edn., vol. 6, Academic Press, New York,
    1962, pp. 95-113.
    2
    Kornberg, A. and Pricer, W.E. Enzymatic phosphorylation of adenosine
    and 2,6-diaminopurine riboside. J. Biol. Chem. 193 (1951) 481-495.
    3
    Kubowitz, F. and Ott, P. Isolierung von Garungsfermenten aus
    menschlichen Muskeln. Biochem. Z. 317 (1944) 193-203.
    4
    Strominger, J.L. Enzymatic synthesis of guanosine and cytidine
    triphosphates: a note on the nucleotide specificity of the pyruvate
    phosphokinase reaction. Biochim. Biophys. Acta 16 (1955) 616-618.
    5
    Tietz, A. and Ochoa, S. "Fluorokinase" and pyruvic kinase. Arch .
    Biochem. Biophys. 78 (1958) 477-493.
  • SMS 2 (Sequence Manipulation Suite , http://bioinformatics.org/sms2/ ) kasutamine (vähemalt 10 manipulatsiooni ):
  • Salvestada leitud E.coli ja H.sapiensi geenide ja valkude järjestused arvutisse FASTA formaadis failidena.
  • Failiformaatide konverteerimine (FASTA formaadis nukleotiidsete ja aminohappeliste järjestuste väljasõelumine GenBanki jt formaadist, aminohapete järjestuse muutmine 1-tähelistest koodidest 3-tähelisteks ja vastupidi, valkude ja DNA järjestuste filtreerimine, järjestuse teatud piirkondade väljavõtmine).
  • Koodonite kasutussageduse uurimine , mustrite otsimine järjestuses, nukleotiidide ja aminohapete sisaldused järjestustes, ORF-de leidmine, PCR produktide otsimine, valgu isoelektrilise punkti arvutamine, valgu molekulmassi arvutamine, restriktsioonisaitide kindlakstegemine , (de)transleerimine, restriktsioonikaartide tegemine, translatsioonikaardid, praimerikaardid.
    Aldolaasi E. coli nukleotiidne järjestus:
    >eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I
    atgacagatattgcgcagttgcttggcaaagacgccgacaaccttttacagcaccgttgt
    atgacaattccttctgaccagctttatctccccggacatgactacgtagaccgcgtaatg
    attgacaataatcgcccgccagcggtgttacgtaatatgcagacgttgtacaacaccggg
    cgtctggctggcacaggatatctttctattctgccggttgaccagggcgttgagcactct
    gccggagcttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactg
    gcgatcgaagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcg
    cggcgttatgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagt
    tacccgaatacctacgatcaaacgctgtatgccagcgtggagcaggcgttcaacatgggc
    gcggttgcggttggtgcgactatctattttggctcggaagagtcacgtcgccagattgaa
    gaaatttctgcggcttttgaacgtgcgcacgagctgggtatggtgacagtgctgtgggcc
    tatttgcgtaactccgcctttaagaaagatggcgttgattaccatgtttccgccgacctg
    accggtcaggcaaaccatctggcggcaaccatcggtgcagatatcgtcaaacaaaaaatg
    gcggaaaataacggcggctataaagcaattaattacggttacaccgacgatcgtgtttac
    agcaaattgaccagcgaaaacccgattgatctggtgcgttatcagttagctaactgctat
    atgggtcgggctgggttgataaactccggcggtgctgcgggcggtgaaactgacctcagc
    gatgcagtgcgtactgcggttatcaacaaacgcgcaggcggaatggggctgattcttgga
    cgtaaagcgttcaagaaatcgatggctgacggcgtgaaactgattaacgccgtgcaggac
    gtttatctcgatagcaaaattactatcgcctga
    10 manipulatsiooni aldolaasi E. coli nukleotiidse järjestusega:
  • Filter DNA
    > filtered DNA sequence consisting of 1075 bases .
    canActataacaCctataacaNatgacagatattgcgcagttgcttggcaaagacgccga
    caaccttttacagcaccgttgtatgacaattccttctgaccagctttatctccccggaca
    tgactacgtagaccgcgtaatgattgacaataatcgcccgccagcggtgttacgtaatat
    gcagacgttgtacaacaccgggcgtctggctggcacaggatatctttctattctgccggt
    tgaccagggcgttgagcactctgccggagcttcatttgctgctaacccgctctactttga
    cccgaaaaacattgttgaactggcgatcgaagcgggctgtaactgtgtggcgtcaactta
    cggcgtgctggcgtcggtatcgcggcgttatgcgcatcgcattccattcctcgtcaaact
    taatcacaacgagacgctaagttacccgaatacctacgatcaaacgctgtatgccagcgt
    ggagcaggcgttcaacatgggcgcggttgcggttggtgcgactatctattttggctcgga
    agagtcacgtcgccagattgaagaaatttctgcggcttttgaacgtgcgcacgagctggg
    tatggtgacagtgctgtgggcctatttgcgtaactccgcctttaagaaagatggcgttga
    ttaccatgtttccgccgacctgaccggtcaggcaaaccatctggcggcaaccatcggtgc
    agatatcgtcaaacaaaaaatggcggaaaataacggcggctataaagcaattaattacgg
    ttacaccgacgatcgtgtttacagcaaattgaccagcgaaaacccgattgatctggtgcg
    ttatcagttagctaactgctatatgggtcgggctgggttgataaactccggcggtgctgc
    gggcggtgaaactgacctcagcgatgcagtgcgtactgcggttatcaacaaacgcgcagg
    cggaatggggctgattcttggacgtaaagcgttcaagaaatcgatggctgacggcgtgaa
    actgattaacgccgtgcaggacgtttatctcgatagcaaaattactatcgcctga
  • CpG Islands
    CpG Islands results
    Results for 1053 residue sequence "eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)" starting "atgacagata"
    CpG island detected in region 3 to 202 (Obs/Exp = 1.18 and %GC = 50.50)
    CpG island detected in region 18 to 217 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 21 to 220 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 24 to 223 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 25 to 224 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 26 to 225 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 27 to 226 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 28 to 227 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 29 to 228 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 30 to 229 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 31 to 230 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 32 to 231 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 33 to 232 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 34 to 233 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 35 to 234 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 36 to 235 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 37 to 236 (Obs/Exp = 1.10 and %GC = 50.50)
    CpG island detected in region 38 to 237 (Obs/Exp = 1.11 and %GC = 50.50)
    CpG island detected in region 39 to 238 (Obs/Exp = 1.11 and %GC = 50.50)
    CpG island detected in region 40 to 239 (Obs/Exp = 1.11 and %GC = 50.50)
    CpG island detected in region 41 to 240 (Obs/Exp = 1.11 and %GC = 50.50)
    CpG island detected in region 42 to 241 (Obs/Exp = 1.08 and %GC = 51.00)
    CpG island detected in region 43 to 242 (Obs/Exp = 1.08 and %GC = 51.00)
    CpG island detected in region 44 to 243 (Obs/Exp = 1.08 and %GC = 51.00)
    CpG island detected in region 45 to 244 (Obs/Exp = 1.14 and %GC = 51.50)
    CpG island detected in region 46 to 245 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 47 to 246 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 48 to 247 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 49 to 248 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 50 to 249 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 51 to 250 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 52 to 251 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 53 to 252 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 54 to 253 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 55 to 254 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 56 to 255 (Obs/Exp = 1.08 and %GC = 51.00)
    CpG island detected in region 57 to 256 (Obs/Exp = 1.08 and %GC = 51.00)
    CpG island detected in region 58 to 257 (Obs/Exp = 1.06 and %GC = 51.50)
    CpG island detected in region 59 to 258 (Obs/Exp = 1.06 and %GC = 51.50)
    CpG island detected in region 60 to 259 (Obs/Exp = 1.06 and %GC = 51.50)
    CpG island detected in region 61 to 260 (Obs/Exp = 1.04 and %GC = 52.00)
    CpG island detected in region 62 to 261 (Obs/Exp = 1.04 and %GC = 52.00)
    CpG island detected in region 63 to 262 (Obs/Exp = 1.04 and %GC = 52.00)
    CpG island detected in region 64 to 263 (Obs/Exp = 1.06 and %GC = 51.50)
    CpG island detected in region 65 to 264 (Obs/Exp = 1.04 and %GC = 52.00)
    CpG island detected in region 66 to 265 (Obs/Exp = 1.04 and %GC = 52.00)
    CpG island detected in region 67 to 266 (Obs/Exp = 1.02 and %GC = 52.50)
    CpG island detected in region 68 to 267 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 69 to 268 (Obs/Exp = 1.06 and %GC = 53.50)
    CpG island detected in region 70 to 269 (Obs/Exp = 1.06 and %GC = 53.50)
    CpG island detected in region 71 to 270 (Obs/Exp = 1.06 and %GC = 53.50)
    CpG island detected in region 72 to 271 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 73 to 272 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 74 to 273 (Obs/Exp = 1.06 and %GC = 53.50)
    CpG island detected in region 75 to 274 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 76 to 275 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 77 to 276 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 78 to 277 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 79 to 278 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 80 to 279 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 81 to 280 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 82 to 281 (Obs/Exp = 1.08 and %GC = 53.00)
    CpG island detected in region 83 to 282 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 84 to 283 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 85 to 284 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 86 to 285 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 87 to 286 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 88 to 287 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 89 to 288 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 90 to 289 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 91 to 290 (Obs/Exp = 1.17 and %GC = 52.50)
    CpG island detected in region 92 to 291 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 93 to 292 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 94 to 293 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 95 to 294 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 96 to 295 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 97 to 296 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 98 to 297 (Obs/Exp = 1.18 and %GC = 50.50)
    CpG island detected in region 99 to 298 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 100 to 299 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 101 to 300 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 102 to 301 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 103 to 302 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 104 to 303 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 105 to 304 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 106 to 305 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 107 to 306 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 108 to 307 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 109 to 308 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 110 to 309 (Obs/Exp = 1.21 and %GC = 51.50)
    CpG island detected in region 111 to 310 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 112 to 311 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 113 to 312 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 114 to 313 (Obs/Exp = 1.18 and %GC = 52.00)
    CpG island detected in region 115 to 314 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 116 to 315 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 117 to 316 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 118 to 317 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 119 to 318 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 120 to 319 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 121 to 320 (Obs/Exp = 1.11 and %GC = 52.00)
    CpG island detected in region 122 to 321 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 123 to 322 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 124 to 323 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 125 to 324 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 126 to 325 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 127 to 326 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 128 to 327 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 129 to 328 (Obs/Exp = 1.05 and %GC = 53.50)
    CpG island detected in region 130 to 329 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 131 to 330 (Obs/Exp = 1.08 and %GC = 54.50)
    CpG island detected in region 132 to 331 (Obs/Exp = 1.08 and %GC = 54.50)
    CpG island detected in region 133 to 332 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 134 to 333 (Obs/Exp = 1.01 and %GC = 54.50)
    CpG island detected in region 135 to 334 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 136 to 335 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 137 to 336 (Obs/Exp = 1.05 and %GC = 53.50)
    CpG island detected in region 138 to 337 (Obs/Exp = 1.00 and %GC = 53.00)
    CpG island detected in region 139 to 338 (Obs/Exp = 1.02 and %GC = 52.50)
    CpG island detected in region 140 to 339 (Obs/Exp = 1.02 and %GC = 52.50)
    CpG island detected in region 141 to 340 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 142 to 341 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 143 to 342 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 144 to 343 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 145 to 344 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 146 to 345 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 147 to 346 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 148 to 347 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 149 to 348 (Obs/Exp = 1.07 and %GC = 53.00)
    CpG island detected in region 150 to 349 (Obs/Exp = 1.05 and %GC = 53.50)
    CpG island detected in region 151 to 350 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 152 to 351 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 153 to 352 (Obs/Exp = 1.05 and %GC = 53.50)
    CpG island detected in region 154 to 353 (Obs/Exp = 1.03 and %GC = 54.00)
    CpG island detected in region 155 to 354 (Obs/Exp = 1.08 and %GC = 54.50)
    CpG island detected in region 156 to 355 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 157 to 356 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 158 to 357 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 159 to 358 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 160 to 359 (Obs/Exp = 1.06 and %GC = 55.00)
    CpG island detected in region 161 to 360 (Obs/Exp = 1.13 and %GC = 55.00)
    CpG island detected in region 162 to 361 (Obs/Exp = 1.11 and %GC = 55.50)
    CpG island detected in region 163 to 362 (Obs/Exp = 1.17 and %GC = 55.50)
    CpG island detected in region 164 to 363 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 165 to 364 (Obs/Exp = 1.09 and %GC = 56.00)
    CpG island detected in region 166 to 365 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 167 to 366 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 168 to 367 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 169 to 368 (Obs/Exp = 1.17 and %GC = 55.50)
    CpG island detected in region 170 to 369 (Obs/Exp = 1.17 and %GC = 55.50)
    CpG island detected in region 171 to 370 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 172 to 371 (Obs/Exp = 1.15 and %GC = 56.00)
    CpG island detected in region 173 to 372 (Obs/Exp = 1.19 and %GC = 56.50)
    CpG island detected in region 174 to 373 (Obs/Exp = 1.17 and %GC = 57.00)
    CpG island detected in region 175 to 374 (Obs/Exp = 1.19 and %GC = 56.50)
    CpG island detected in region 176 to 375 (Obs/Exp = 1.19 and %GC = 56.50)
    CpG island detected in region 177 to 376 (Obs/Exp = 1.19 and %GC = 56.50)
    CpG island detected in region 178 to 377 (Obs/Exp = 1.20 and %GC = 56.50)
    CpG island detected in region 179 to 378 (Obs/Exp = 1.19 and %GC = 56.50)
    CpG island detected in region 180 to 379 (Obs/Exp = 1.22 and %GC = 56.00)
    CpG island detected in region 181 to 380 (Obs/Exp = 1.24 and %GC = 55.50)
    CpG island detected in region 182 to 381 (Obs/Exp = 1.19 and %GC = 55.00)
    CpG island detected in region 183 to 382 (Obs/Exp = 1.19 and %GC = 55.00)
    CpG island detected in region 184 to 383 (Obs/Exp = 1.17 and %GC = 55.50)
    CpG island detected in region 185 to 384 (Obs/Exp = 1.19 and %GC = 55.00)
    CpG island detected in region 186 to 385 (Obs/Exp = 1.19 and %GC = 55.00)
    CpG island detected in region 187 to 386 (Obs/Exp = 1.21 and %GC = 54.50)
    CpG island detected in region 188 to 387 (Obs/Exp = 1.21 and %GC = 54.50)
    CpG island detected in region 189 to 388 (Obs/Exp = 1.21 and %GC = 54.50)
    CpG island detected in region 190 to 389 (Obs/Exp = 1.21 and %GC = 54.50)
    CpG island detected in region 191 to 390 (Obs/Exp = 1.21 and %GC = 54.50)
    CpG island detected in region 192 to 391 (Obs/Exp = 1.28 and %GC = 54.50)
    CpG island detected in region 193 to 392 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 194 to 393 (Obs/Exp = 1.28 and %GC = 54.50)
    CpG island detected in region 195 to 394 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 196 to 395 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 197 to 396 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 198 to 397 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 199 to 398 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 200 to 399 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 201 to 400 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 202 to 401 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 203 to 402 (Obs/Exp = 1.35 and %GC = 53.00)
    CpG island detected in region 204 to 403 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 205 to 404 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 206 to 405 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 207 to 406 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 208 to 407 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 209 to 408 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 210 to 409 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 211 to 410 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 212 to 411 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 213 to 412 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 214 to 413 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 215 to 414 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 216 to 415 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 217 to 416 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 218 to 417 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 219 to 418 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 220 to 419 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 221 to 420 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 222 to 421 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 223 to 422 (Obs/Exp = 1.42 and %GC = 53.00)
    CpG island detected in region 224 to 423 (Obs/Exp = 1.42 and %GC = 53.00)
    CpG island detected in region 225 to 424 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 226 to 425 (Obs/Exp = 1.40 and %GC = 53.50)
    CpG island detected in region 227 to 426 (Obs/Exp = 1.47 and %GC = 53.50)
    CpG island detected in region 228 to 427 (Obs/Exp = 1.50 and %GC = 53.00)
    CpG island detected in region 229 to 428 (Obs/Exp = 1.45 and %GC = 52.50)
    CpG island detected in region 230 to 429 (Obs/Exp = 1.48 and %GC = 52.00)
    CpG island detected in region 231 to 430 (Obs/Exp = 1.48 and %GC = 52.00)
    CpG island detected in region 232 to 431 (Obs/Exp = 1.46 and %GC = 52.50)
    CpG island detected in region 233 to 432 (Obs/Exp = 1.46 and %GC = 52.50)
    CpG island detected in region 234 to 433 (Obs/Exp = 1.46 and %GC = 52.50)
    CpG island detected in region 235 to 434 (Obs/Exp = 1.49 and %GC = 52.00)
    CpG island detected in region 236 to 435 (Obs/Exp = 1.49 and %GC = 52.00)
    CpG island detected in region 237 to 436 (Obs/Exp = 1.54 and %GC = 52.50)
    CpG island detected in region 238 to 437 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 239 to 438 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 240 to 439 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 241 to 440 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 242 to 441 (Obs/Exp = 1.60 and %GC = 51.50)
    CpG island detected in region 243 to 442 (Obs/Exp = 1.62 and %GC = 51.00)
    CpG island detected in region 244 to 443 (Obs/Exp = 1.55 and %GC = 51.00)
    CpG island detected in region 245 to 444 (Obs/Exp = 1.62 and %GC = 51.00)
    CpG island detected in region 246 to 445 (Obs/Exp = 1.63 and %GC = 51.00)
    CpG island detected in region 247 to 446 (Obs/Exp = 1.63 and %GC = 51.00)
    CpG island detected in region 248 to 447 (Obs/Exp = 1.63 and %GC = 51.00)
    CpG island detected in region 249 to 448 (Obs/Exp = 1.66 and %GC = 50.50)
    CpG island detected in region 250 to 449 (Obs/Exp = 1.66 and %GC = 50.50)
    CpG island detected in region 251 to 450 (Obs/Exp = 1.66 and %GC = 50.50)
    CpG island detected in region 252 to 451 (Obs/Exp = 1.65 and %GC = 50.50)
    CpG island detected in region 253 to 452 (Obs/Exp = 1.62 and %GC = 51.00)
    CpG island detected in region 254 to 453 (Obs/Exp = 1.60 and %GC = 51.50)
    CpG island detected in region 255 to 454 (Obs/Exp = 1.60 and %GC = 51.50)
    CpG island detected in region 256 to 455 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 257 to 456 (Obs/Exp = 1.57 and %GC = 52.00)
    CpG island detected in region 258 to 457 (Obs/Exp = 1.64 and %GC = 52.00)
    CpG island detected in region 259 to 458 (Obs/Exp = 1.64 and %GC = 52.00)
    CpG island detected in region 260 to 459 (Obs/Exp = 1.64 and %GC = 52.00)
    CpG island detected in region 261 to 460 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 262 to 461 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 263 to 462 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 264 to 463 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 265 to 464 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 266 to 465 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 267 to 466 (Obs/Exp = 1.52 and %GC = 52.50)
    CpG island detected in region 268 to 467 (Obs/Exp = 1.53 and %GC = 52.50)
    CpG island detected in region 269 to 468 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 270 to 469 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 271 to 470 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 272 to 471 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 273 to 472 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 274 to 473 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 275 to 474 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 276 to 475 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 277 to 476 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 278 to 477 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 279 to 478 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 280 to 479 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 281 to 480 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 282 to 481 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 283 to 482 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 284 to 483 (Obs/Exp = 1.61 and %GC = 53.50)
    CpG island detected in region 285 to 484 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 286 to 485 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 287 to 486 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 288 to 487 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 289 to 488 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 290 to 489 (Obs/Exp = 1.59 and %GC = 55.00)
    CpG island detected in region 291 to 490 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 292 to 491 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 293 to 492 (Obs/Exp = 1.59 and %GC = 55.00)
    CpG island detected in region 294 to 493 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 295 to 494 (Obs/Exp = 1.54 and %GC = 56.00)
    CpG island detected in region 296 to 495 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 297 to 496 (Obs/Exp = 1.54 and %GC = 56.00)
    CpG island detected in region 298 to 497 (Obs/Exp = 1.51 and %GC = 56.50)
    CpG island detected in region 299 to 498 (Obs/Exp = 1.58 and %GC = 56.50)
    CpG island detected in region 300 to 499 (Obs/Exp = 1.58 and %GC = 56.50)
    CpG island detected in region 301 to 500 (Obs/Exp = 1.57 and %GC = 56.50)
    CpG island detected in region 302 to 501 (Obs/Exp = 1.60 and %GC = 56.00)
    CpG island detected in region 303 to 502 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 304 to 503 (Obs/Exp = 1.59 and %GC = 55.00)
    CpG island detected in region 305 to 504 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 306 to 505 (Obs/Exp = 1.56 and %GC = 55.50)
    CpG island detected in region 307 to 506 (Obs/Exp = 1.53 and %GC = 55.00)
    CpG island detected in region 308 to 507 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 309 to 508 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 310 to 509 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 311 to 510 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 312 to 511 (Obs/Exp = 1.51 and %GC = 54.00)
    CpG island detected in region 313 to 512 (Obs/Exp = 1.51 and %GC = 54.00)
    CpG island detected in region 314 to 513 (Obs/Exp = 1.51 and %GC = 54.00)
    CpG island detected in region 315 to 514 (Obs/Exp = 1.54 and %GC = 53.50)
    CpG island detected in region 316 to 515 (Obs/Exp = 1.54 and %GC = 53.50)
    CpG island detected in region 317 to 516 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 318 to 517 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 319 to 518 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 320 to 519 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 321 to 520 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 322 to 521 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 323 to 522 (Obs/Exp = 1.56 and %GC = 54.50)
    CpG island detected in region 324 to 523 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 325 to 524 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 326 to 525 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 327 to 526 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 328 to 527 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 329 to 528 (Obs/Exp = 1.65 and %GC = 54.00)
    CpG island detected in region 330 to 529 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 331 to 530 (Obs/Exp = 1.65 and %GC = 54.00)
    CpG island detected in region 332 to 531 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 333 to 532 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 334 to 533 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 335 to 534 (Obs/Exp = 1.59 and %GC = 55.00)
    CpG island detected in region 336 to 535 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 337 to 536 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 338 to 537 (Obs/Exp = 1.62 and %GC = 54.50)
    CpG island detected in region 339 to 538 (Obs/Exp = 1.59 and %GC = 55.00)
    CpG island detected in region 340 to 539 (Obs/Exp = 1.55 and %GC = 54.50)
    CpG island detected in region 341 to 540 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 342 to 541 (Obs/Exp = 1.58 and %GC = 54.00)
    CpG island detected in region 343 to 542 (Obs/Exp = 1.54 and %GC = 53.50)
    CpG island detected in region 344 to 543 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 345 to 544 (Obs/Exp = 1.57 and %GC = 53.00)
    CpG island detected in region 346 to 545 (Obs/Exp = 1.60 and %GC = 52.50)
    CpG island detected in region 347 to 546 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 348 to 547 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 349 to 548 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 350 to 549 (Obs/Exp = 1.66 and %GC = 51.50)
    CpG island detected in region 351 to 550 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 352 to 551 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 353 to 552 (Obs/Exp = 1.63 and %GC = 52.00)
    CpG island detected in region 354 to 553 (Obs/Exp = 1.56 and %GC = 52.00)
    CpG island detected in region 355 to 554 (Obs/Exp = 1.55 and %GC = 52.00)
    CpG island detected in region 356 to 555 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 357 to 556 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 358 to 557 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 359 to 558 (Obs/Exp = 1.58 and %GC = 51.50)
    CpG island detected in region 360 to 559 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 361 to 560 (Obs/Exp = 1.54 and %GC = 51.00)
    CpG island detected in region 362 to 561 (Obs/Exp = 1.49 and %GC = 50.50)
    CpG island detected in region 363 to 562 (Obs/Exp = 1.49 and %GC = 50.50)
    CpG island detected in region 364 to 563 (Obs/Exp = 1.57 and %GC = 50.50)
    CpG island detected in region 367 to 566 (Obs/Exp = 1.49 and %GC = 50.50)
    CpG island detected in region 368 to 567 (Obs/Exp = 1.54 and %GC = 51.00)
    CpG island detected in region 369 to 568 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 370 to 569 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 371 to 570 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 372 to 571 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 373 to 572 (Obs/Exp = 1.54 and %GC = 51.00)
    CpG island detected in region 374 to 573 (Obs/Exp = 1.54 and %GC = 51.00)
    CpG island detected in region 375 to 574 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 376 to 575 (Obs/Exp = 1.51 and %GC = 51.50)
    CpG island detected in region 377 to 576 (Obs/Exp = 1.43 and %GC = 51.50)
    CpG island detected in region 378 to 577 (Obs/Exp = 1.43 and %GC = 51.50)
    CpG island detected in region 379 to 578 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 380 to 579 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 381 to 580 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 382 to 581 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 383 to 582 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 384 to 583 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 385 to 584 (Obs/Exp = 1.44 and %GC = 51.50)
    CpG island detected in region 386 to 585 (Obs/Exp = 1.42 and %GC = 52.00)
    CpG island detected in region 387 to 586 (Obs/Exp = 1.42 and %GC = 52.00)
    CpG island detected in region 388 to 587 (Obs/Exp = 1.42 and %GC = 52.00)
    CpG island detected in region 389 to 588 (Obs/Exp = 1.45 and %GC = 51.50)
    CpG island detected in region 390 to 589 (Obs/Exp = 1.42 and %GC = 52.00)
    CpG island detected in region 391 to 590 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 392 to 591 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 393 to 592 (Obs/Exp = 1.35 and %GC = 52.00)
    CpG island detected in region 394 to 593 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 395 to 594 (Obs/Exp = 1.36 and %GC = 52.00)
    CpG island detected in region 396 to 595 (Obs/Exp = 1.36 and %GC = 52.00)
    CpG island detected in region 397 to 596 (Obs/Exp = 1.33 and %GC = 52.50)
    CpG island detected in region 398 to 597 (Obs/Exp = 1.34 and %GC = 52.50)
    CpG island detected in region 399 to 598 (Obs/Exp = 1.32 and %GC = 53.00)
    CpG island detected in region 400 to 599 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 401 to 600 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 402 to 601 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 403 to 602 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 404 to 603 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 405 to 604 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 406 to 605 (Obs/Exp = 1.32 and %GC = 53.00)
    CpG island detected in region 407 to 606 (Obs/Exp = 1.30 and %GC = 53.50)
    CpG island detected in region 408 to 607 (Obs/Exp = 1.27 and %GC = 54.00)
    CpG island detected in region 409 to 608 (Obs/Exp = 1.28 and %GC = 54.00)
    CpG island detected in region 410 to 609 (Obs/Exp = 1.30 and %GC = 53.50)
    CpG island detected in region 411 to 610 (Obs/Exp = 1.30 and %GC = 53.50)
    CpG island detected in region 412 to 611 (Obs/Exp = 1.32 and %GC = 53.00)
    CpG island detected in region 413 to 612 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 414 to 613 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 415 to 614 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 416 to 615 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 417 to 616 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 418 to 617 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 419 to 618 (Obs/Exp = 1.24 and %GC = 54.50)
    CpG island detected in region 420 to 619 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 421 to 620 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 422 to 621 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 423 to 622 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 424 to 623 (Obs/Exp = 1.28 and %GC = 53.50)
    CpG island detected in region 425 to 624 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 426 to 625 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 427 to 626 (Obs/Exp = 1.27 and %GC = 52.50)
    CpG island detected in region 428 to 627 (Obs/Exp = 1.27 and %GC = 52.50)
    CpG island detected in region 429 to 628 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 430 to 629 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 431 to 630 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 432 to 631 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 433 to 632 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 434 to 633 (Obs/Exp = 1.24 and %GC = 53.50)
    CpG island detected in region 435 to 634 (Obs/Exp = 1.29 and %GC = 54.00)
    CpG island detected in region 436 to 635 (Obs/Exp = 1.25 and %GC = 53.50)
    CpG island detected in region 437 to 636 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 438 to 637 (Obs/Exp = 1.25 and %GC = 53.50)
    CpG island detected in region 439 to 638 (Obs/Exp = 1.25 and %GC = 53.50)
    CpG island detected in region 440 to 639 (Obs/Exp = 1.28 and %GC = 53.00)
    CpG island detected in region 441 to 640 (Obs/Exp = 1.28 and %GC = 53.00)
    CpG island detected in region 442 to 641 (Obs/Exp = 1.28 and %GC = 53.00)
    CpG island detected in region 443 to 642 (Obs/Exp = 1.25 and %GC = 53.50)
    CpG island detected in region 444 to 643 (Obs/Exp = 1.17 and %GC = 53.50)
    CpG island detected in region 445 to 644 (Obs/Exp = 1.19 and %GC = 53.00)
    CpG island detected in region 446 to 645 (Obs/Exp = 1.22 and %GC = 52.50)
    CpG island detected in region 447 to 646 (Obs/Exp = 1.20 and %GC = 53.00)
    CpG island detected in region 448 to 647 (Obs/Exp = 1.22 and %GC = 52.50)
    CpG island detected in region 449 to 648 (Obs/Exp = 1.22 and %GC = 52.50)
    CpG island detected in region 450 to 649 (Obs/Exp = 1.22 and %GC = 52.50)
    CpG island detected in region 451 to 650 (Obs/Exp = 1.19 and %GC = 53.00)
    CpG island detected in region 452 to 651 (Obs/Exp = 1.18 and %GC = 53.00)
    CpG island detected in region 453 to 652 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 454 to 653 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 455 to 654 (Obs/Exp = 1.24 and %GC = 53.50)
    CpG island detected in region 456 to 655 (Obs/Exp = 1.31 and %GC = 53.50)
    CpG island detected in region 457 to 656 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 458 to 657 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 459 to 658 (Obs/Exp = 1.23 and %GC = 53.50)
    CpG island detected in region 460 to 659 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 461 to 660 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 462 to 661 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 463 to 662 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 464 to 663 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 465 to 664 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 466 to 665 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 467 to 666 (Obs/Exp = 1.31 and %GC = 53.00)
    CpG island detected in region 468 to 667 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 469 to 668 (Obs/Exp = 1.26 and %GC = 52.50)
    CpG island detected in region 470 to 669 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 471 to 670 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 472 to 671 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 473 to 672 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 474 to 673 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 475 to 674 (Obs/Exp = 1.25 and %GC = 53.00)
    CpG island detected in region 476 to 675 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 477 to 676 (Obs/Exp = 1.19 and %GC = 54.00)
    CpG island detected in region 478 to 677 (Obs/Exp = 1.21 and %GC = 53.50)
    CpG island detected in region 479 to 678 (Obs/Exp = 1.23 and %GC = 53.00)
    CpG island detected in region 480 to 679 (Obs/Exp = 1.23 and %GC = 53.00)
    CpG island detected in region 481 to 680 (Obs/Exp = 1.18 and %GC = 52.50)
    CpG island detected in region 482 to 681 (Obs/Exp = 1.18 and %GC = 52.50)
    CpG island detected in region 483 to 682 (Obs/Exp = 1.11 and %GC = 52.50)
    CpG island detected in region 484 to 683 (Obs/Exp = 1.11 and %GC = 52.50)
    CpG island detected in region 485 to 684 (Obs/Exp = 1.18 and %GC = 52.50)
    CpG island detected in region 486 to 685 (Obs/Exp = 1.16 and %GC = 53.00)
    CpG island detected in region 487 to 686 (Obs/Exp = 1.13 and %GC = 53.50)
    CpG island detected in region 488 to 687 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 489 to 688 (Obs/Exp = 1.11 and %GC = 52.50)
    CpG island detected in region 490 to 689 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 491 to 690 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 492 to 691 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 493 to 692 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 494 to 693 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 495 to 694 (Obs/Exp = 1.17 and %GC = 52.50)
    CpG island detected in region 496 to 695 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 497 to 696 (Obs/Exp = 1.17 and %GC = 52.50)
    CpG island detected in region 498 to 697 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 499 to 698 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 500 to 699 (Obs/Exp = 1.09 and %GC = 52.50)
    CpG island detected in region 501 to 700 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 502 to 701 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 503 to 702 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 504 to 703 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 505 to 704 (Obs/Exp = 1.12 and %GC = 52.00)
    CpG island detected in region 506 to 705 (Obs/Exp = 1.10 and %GC = 52.50)
    CpG island detected in region 507 to 706 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 508 to 707 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 509 to 708 (Obs/Exp = 1.13 and %GC = 53.50)
    CpG island detected in region 510 to 709 (Obs/Exp = 1.13 and %GC = 53.50)
    CpG island detected in region 511 to 710 (Obs/Exp = 1.13 and %GC = 53.50)
    CpG island detected in region 512 to 711 (Obs/Exp = 1.15 and %GC = 53.00)
    CpG island detected in region 513 to 712 (Obs/Exp = 1.14 and %GC = 53.00)
    CpG island detected in region 514 to 713 (Obs/Exp = 1.17 and %GC = 52.50)
    CpG island detected in region 515 to 714 (Obs/Exp = 1.17 and %GC = 52.50)
    CpG island detected in region 516 to 715 (Obs/Exp = 1.12 and %GC = 52.00)
    CpG island detected in region 517 to 716 (Obs/Exp = 1.14 and %GC = 51.50)
    CpG island detected in region 518 to 717 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 519 to 718 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 520 to 719 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 521 to 720 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 522 to 721 (Obs/Exp = 1.14 and %GC = 51.50)
    CpG island detected in region 523 to 722 (Obs/Exp = 1.13 and %GC = 51.50)
    CpG island detected in region 524 to 723 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 525 to 724 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 526 to 725 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 527 to 726 (Obs/Exp = 1.14 and %GC = 51.50)
    CpG island detected in region 528 to 727 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 529 to 728 (Obs/Exp = 1.16 and %GC = 51.00)
    CpG island detected in region 530 to 729 (Obs/Exp = 1.11 and %GC = 50.50)
    CpG island detected in region 536 to 735 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 537 to 736 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 538 to 737 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 539 to 738 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 540 to 739 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 541 to 740 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 542 to 741 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 543 to 742 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 544 to 743 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 545 to 744 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 546 to 745 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 547 to 746 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 548 to 747 (Obs/Exp = 1.19 and %GC = 52.00)
    CpG island detected in region 549 to 748 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 550 to 749 (Obs/Exp = 1.22 and %GC = 51.50)
    CpG island detected in region 551 to 750 (Obs/Exp = 1.24 and %GC = 51.00)
    CpG island detected in region 552 to 751 (Obs/Exp = 1.19 and %GC = 50.50)
    CpG island detected in region 559 to 758 (Obs/Exp = 1.26 and %GC = 50.50)
    CpG island detected in region 718 to 917 (Obs/Exp = 1.36 and %GC = 50.50)
    CpG island detected in region 719 to 918 (Obs/Exp = 1.42 and %GC = 51.00)
    CpG island detected in region 720 to 919 (Obs/Exp = 1.40 and %GC = 51.50)
    CpG island detected in region 721 to 920 (Obs/Exp = 1.42 and %GC = 51.00)
    CpG island detected in region 722 to 921 (Obs/Exp = 1.44 and %GC = 50.50)
    CpG island detected in region 732 to 931 (Obs/Exp = 1.35 and %GC = 50.50)
    CpG island detected in region 733 to 932 (Obs/Exp = 1.36 and %GC = 50.50)
    CpG island detected in region 734 to 933 (Obs/Exp = 1.35 and %GC = 50.50)
    CpG island detected in region 735 to 934 (Obs/Exp = 1.43 and %GC = 50.50)
    CpG island detected in region 736 to 935 (Obs/Exp = 1.35 and %GC = 50.50)
    CpG island detected in region 740 to 939 (Obs/Exp = 1.35 and %GC = 50.50)
    CpG island detected in region 741 to 940 (Obs/Exp = 1.40 and %GC = 51.00)
    CpG island detected in region 742 to 941 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 743 to 942 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 744 to 943 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 745 to 944 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 746 to 945 (Obs/Exp = 1.38 and %GC = 51.50)
    CpG island detected in region 747 to 946 (Obs/Exp = 1.39 and %GC = 51.50)
    CpG island detected in region 748 to 947 (Obs/Exp = 1.36 and %GC = 52.00)
    CpG island detected in region 749 to 948 (Obs/Exp = 1.34 and %GC = 52.50)
    CpG island detected in region 750 to 949 (Obs/Exp = 1.31 and %GC = 53.00)
    CpG island detected in region 751 to 950 (Obs/Exp = 1.31 and %GC = 53.00)
    CpG island detected in region 752 to 951 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 753 to 952 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 754 to 953 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 755 to 954 (Obs/Exp = 1.29 and %GC = 53.50)
    CpG island detected in region 756 to 955 (Obs/Exp = 1.26 and %GC = 54.00)
    CpG island detected in region 757 to 956 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 758 to 957 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 759 to 958 (Obs/Exp = 1.24 and %GC = 53.00)
    CpG island detected in region 760 to 959 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 761 to 960 (Obs/Exp = 1.22 and %GC = 53.50)
    CpG island detected in region 762 to 961 (Obs/Exp = 1.19 and %GC = 54.00)
    CpG island detected in region 763 to 962 (Obs/Exp = 1.27 and %GC = 54.00)
    CpG island detected in region 764 to 963 (Obs/Exp = 1.27 and %GC = 54.00)
    CpG island detected in region 765 to 964 (Obs/Exp = 1.30 and %GC = 53.50)
    CpG island detected in region 766 to 965 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 767 to 966 (Obs/Exp = 1.28 and %GC = 52.50)
    CpG island detected in region 768 to 967 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 769 to 968 (Obs/Exp = 1.18 and %GC = 53.00)
    CpG island detected in region 770 to 969 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 771 to 970 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 772 to 971 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 773 to 972 (Obs/Exp = 1.18 and %GC = 53.00)
    CpG island detected in region 774 to 973 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 775 to 974 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 776 to 975 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 777 to 976 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 778 to 977 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 779 to 978 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 780 to 979 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 781 to 980 (Obs/Exp = 1.20 and %GC = 52.50)
    CpG island detected in region 782 to 981 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 783 to 982 (Obs/Exp = 1.28 and %GC = 52.50)
    CpG island detected in region 784 to 983 (Obs/Exp = 1.31 and %GC = 52.00)
    CpG island detected in region 785 to 984 (Obs/Exp = 1.28 and %GC = 52.50)
    CpG island detected in region 786 to 985 (Obs/Exp = 1.26 and %GC = 53.00)
    CpG island detected in region 787 to 986 (Obs/Exp = 1.24 and %GC = 53.50)
    CpG island detected in region 788 to 987 (Obs/Exp = 1.24 and %GC = 53.50)
    CpG island detected in region 789 to 988 (Obs/Exp = 1.22 and %GC = 54.00)
    CpG island detected in region 790 to 989 (Obs/Exp = 1.24 and %GC = 53.50)
    CpG island detected in region 791 to 990 (Obs/Exp = 1.21 and %GC = 54.00)
    CpG island detected in region 792 to 991 (Obs/Exp = 1.29 and %GC = 54.00)
    CpG island detected in region 793 to 992 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 794 to 993 (Obs/Exp = 1.27 and %GC = 54.50)
    CpG island detected in region 795 to 994 (Obs/Exp = 1.34 and %GC = 54.50)
    CpG island detected in region 796 to 995 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 797 to 996 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 798 to 997 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 799 to 998 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 800 to 999 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 801 to 1000 (Obs/Exp = 1.27 and %GC = 54.50)
    CpG island detected in region 802 to 1001 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 803 to 1002 (Obs/Exp = 1.31 and %GC = 54.00)
    CpG island detected in region 804 to 1003 (Obs/Exp = 1.27 and %GC = 53.50)
    CpG island detected in region 805 to 1004 (Obs/Exp = 1.29 and %GC = 53.00)
    CpG island detected in region 806 to 1005 (Obs/Exp = 1.29 and %GC = 53.00)
    CpG island detected in region 807 to 1006 (Obs/Exp = 1.29 and %GC = 53.00)
    CpG island detected in region 808 to 1007 (Obs/Exp = 1.29 and %GC = 53.00)
    CpG island detected in region 809 to 1008 (Obs/Exp = 1.28 and %GC = 53.00)
    CpG island detected in region 810 to 1009 (Obs/Exp = 1.33 and %GC = 53.50)
    CpG island detected in region 811 to 1010 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 812 to 1011 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 813 to 1012 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 814 to 1013 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 815 to 1014 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 816 to 1015 (Obs/Exp = 1.34 and %GC = 54.50)
    CpG island detected in region 817 to 1016 (Obs/Exp = 1.36 and %GC = 54.00)
    CpG island detected in region 818 to 1017 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 819 to 1018 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 820 to 1019 (Obs/Exp = 1.30 and %GC = 54.00)
    CpG island detected in region 821 to 1020 (Obs/Exp = 1.27 and %GC = 54.50)
    CpG island detected in region 822 to 1021 (Obs/Exp = 1.32 and %GC = 55.00)
    CpG island detected in region 823 to 1022 (Obs/Exp = 1.32 and %GC = 55.00)
    CpG island detected in region 824 to 1023 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 825 to 1024 (Obs/Exp = 1.35 and %GC = 54.50)
    CpG island detected in region 826 to 1025 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 827 to 1026 (Obs/Exp = 1.37 and %GC = 54.00)
    CpG island detected in region 828 to 1027 (Obs/Exp = 1.34 and %GC = 54.50)
    CpG island detected in region 829 to 1028 (Obs/Exp = 1.34 and %GC = 54.50)
    CpG island detected in region 830 to 1029 (Obs/Exp = 1.33 and %GC = 54.50)
    CpG island detected in region 831 to 1030 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 832 to 1031 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 833 to 1032 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 834 to 1033 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 835 to 1034 (Obs/Exp = 1.42 and %GC = 54.50)
    CpG island detected in region 836 to 1035 (Obs/Exp = 1.39 and %GC = 55.00)
    CpG island detected in region 837 to 1036 (Obs/Exp = 1.41 and %GC = 54.50)
    CpG island detected in region 838 to 1037 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 839 to 1038 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 840 to 1039 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 841 to 1040 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 842 to 1041 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 843 to 1042 (Obs/Exp = 1.44 and %GC = 54.00)
    CpG island detected in region 844 to 1043 (Obs/Exp = 1.43 and %GC = 54.00)
    CpG island detected in region 845 to 1044 (Obs/Exp = 1.45 and %GC = 53.50)
    CpG island detected in region 846 to 1045 (Obs/Exp = 1.48 and %GC = 53.00)
    CpG island detected in region 847 to 1046 (Obs/Exp = 1.48 and %GC = 53.00)
    CpG island detected in region 848 to 1047 (Obs/Exp = 1.40 and %GC = 53.00)
    CpG island detected in region 849 to 1048 (Obs/Exp = 1.48 and %GC = 53.00)
    CpG island detected in region 850 to 1049 (Obs/Exp = 1.47 and %GC = 53.00)
    CpG island detected in region 851 to 1050 (Obs/Exp = 1.46 and %GC = 53.00)
    CpG island detected in region 852 to 1051 (Obs/Exp = 1.49 and %GC = 52.50)
    CpG island detected in region 853 to 1052 (Obs/Exp = 1.47 and %GC = 53.00)
    CpG island detected in region 854 to 1053 (Obs/Exp = 1.49 and %GC = 52.50)
  • Mutate for Digest
    Mutate for Digest results
    mutated sequence
    current sequence
    Results for linear 1053 residue sequence "eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)" starting "atgacagata"
    1 M T D I A Q L L G K D A D N L L Q H R C
    1 M T D I A Q L L G K D A D N L L Q H R C
    1 atgacagatattgcgcagttgcttggcaaagacgccgacaaccttttacagcaccgttgt
    1 atgacagatattgcgcagttgcttggcaaagacgccgacaaccttttacagcaccgttgt
    21 M T I P S D Q L Y L P G H D Y V D R V M
    21 M T I P S D Q L Y L P G H D Y V D R V M
    61 atgacaattccttctgaccagctttatctccccggacatgactacgtagaccgcgtaatg
    61 atgacaattccttctgaccagctttatctccccggacatgactacgtagaccgcgtaatg
    41 I D N N R P P A V L R N M Q T L Y N T G
    41 I D N N R P P A V L R N M Q T L Y N T G
    121 attgacaataatcgcccgccagcggtgttacgtaatatgcagacgttgtacaacaccggg
    121 attgacaataatcgcccgccagcggtgttacgtaatatgcagacgttgtacaacaccggg
    61 R L A G T G Y L S I L P V D Q G V E H S
    61 R L A G T G Y L S I L P V D Q G V E H S
    181 cgtctggctggcacaggatatctttctattctgccggttgaccagggcgttgagcactct
    181 cgtctggctggcacaggatatctttctattctgccggttgaccagggcgttgagcactct
    81 A G A S F A A N P L Y F D P K N I V E L
    81 A G A S F A A N P L Y F D P K N I V E L
    241 gccggagcttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactg
    241 gccggagcttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactg
    101 A I E A G C N C V A S T Y G V L A S V S
    101 A I E A G C N C V A S T Y G V L A S V S
    301 gcgatcgaagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcg
    301 gcgatcgaagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcg
    121 R R Y A H R I P F L V K L N H N E T L S
    121 R R Y A H R I P F L V K L N H N E T L S
    361 cggcgttatgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagt
    361 cggcgttatgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagt
    141 Y P N T Y D Q T L Y A S V E Q A F N M G
    141 Y P N T Y D Q T L Y A S V E Q A F N M G
    421 tacccgaatacctacgatcaaacgctgtatgccagcgtggagcaggcgttcaacatgggc
    421 tacccgaatacctacgatcaaacgctgtatgccagcgtggagcaggcgttcaacatgggc
    161 A V A V G A T I Y F G S E E S R R Q I E
    161 A V A V G A T I Y F G S E E S R R Q I E
    481 gcggttgcggttggtgcgactatctattttggctcggaagagtcacgtcgccagattgaa
    481 gcggttgcggttggtgcgactatctattttggctcggaagagtcacgtcgccagattgaa
    181 E I S A A F E R A H E L G M V T V L W A
    181 E I S A A F E R A H E L G M V T V L W A
    541 gaaatttctgcggcttttgaacgtgcgcacgagctgggtatggtgacagtgctgtgggcc
    541 gaaatttctgcggcttttgaacgtgcgcacgagctgggtatggtgacagtgctgtgggcc
    201 Y L R N S A F K K D G V D Y H V S A D L
    201 Y L R N S A F K K D G V D Y H V S A D L
    601 tatttgcgtaactccgcctttaagaaagatggcgttgattaccatgtttccgccgacctg
    601 tatttgcgtaactccgcctttaagaaagatggcgttgattaccatgtttccgccgacctg
    221 T G Q A N H L A A T I G A D I V K Q K M
    221 T G Q A N H L A A T I G A D I V K Q K M
    661 accggtcaggcaaaccatctggcggcaaccatcggtgcagatatcgtcaaacaaaaaatg
    661 accggtcaggcaaaccatctggcggcaaccatcggtgcagatatcgtcaaacaaaaaatg
    241 A E N N G G Y K A I N Y G Y T D D R V Y
    241 A E N N G G Y K A I N Y G Y T D D R V Y
    721 gcggaaaataacggcggctataaagcaattaattacggttacaccgacgatcgtgtttac
    721 gcggaaaataacggcggctataaagcaattaattacggttacaccgacgatcgtgtttac
    261 S K L T S E N P I D L V R Y Q L A N C Y
    261 S K L T S E N P I D L V R Y Q L A N C Y
    781 agcaaattgaccagcgaaaacccgattgatctggtgcgttatcagttagctaactgctat
    781 agcaaattgaccagcgaaaacccgattgatctggtgcgttatcagttagctaactgctat
    281 M G R A G L I N S G G A A G G E T D L S
    281 M G R A G L I N S G G A A G G E T D L S
    841 atgggtcgggctgggttgataaactccggcggtgctgcgggcggtgaaactgacctcagc
    841 atgggtcgggctgggttgataaactccggcggtgctgcgggcggtgaaactgacctcagc
    301 D A V R T A V I N K R A G G M G L I L G
    301 D A V R T A V I N K R A G G M G L I L G
    901 gatgcagtgcgtactgcggttatcaacaaacgcgcaggcggaatggggctgattcttgga
    901 gatgcagtgcgtactgcggttatcaacaaacgcgcaggcggaatggggctgattcttgga
    321 R K A F K K S M A D G V K L I N A V Q D
    321 R K A F K K S M A D G V K L I N A V Q D
    961 cgtaaagcgttcaagaaatcgatggctgacggcgtgaaactgattaacgccgtgcaggac
    961 cgtaaagcgttcaagaaatcgatggctgacggcgtgaaactgattaacgccgtgcaggac
    341 V Y L D S K I T I A *
    341 V Y L D S K I T I A *
    1021 gtttatctcgatagcaaaattactatcgcctga
    1021 gtttatctcgatagcaaaattactatcgcctga
    5) ORF Finder
    ORF Finder results
    Results for 1053 residue sequence "eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)" starting "atgacagata"
    >ORF number 1 in reading frame 1 on the direct strand extends from base 1 to base 1053.
    atgacagatattgcgcagttgcttggcaaagacgccgacaaccttttacagcaccgttgt
    atgacaattccttctgaccagctttatctccccggacatgactacgtagaccgcgtaatg
    attgacaataatcgcccgccagcggtgttacgtaatatgcagacgttgtacaacaccggg
    cgtctggctggcacaggatatctttctattctgccggttgaccagggcgttgagcactct
    gccggagcttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactg
    gcgatcgaagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcg
    cggcgttatgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagt
    tacccgaatacctacgatcaaacgctgtatgccagcgtggagcaggcgttcaacatgggc
    gcggttgcggttggtgcgactatctattttggctcggaagagtcacgtcgccagattgaa
    gaaatttctgcggcttttgaacgtgcgcacgagctgggtatggtgacagtgctgtgggcc
    tatttgcgtaactccgcctttaagaaagatggcgttgattaccatgtttccgccgacctg
    accggtcaggcaaaccatctggcggcaaccatcggtgcagatatcgtcaaacaaaaaatg
    gcggaaaataacggcggctataaagcaattaattacggttacaccgacgatcgtgtttac
    agcaaattgaccagcgaaaacccgattgatctggtgcgttatcagttagctaactgctat
    atgggtcgggctgggttgataaactccggcggtgctgcgggcggtgaaactgacctcagc
    gatgcagtgcgtactgcggttatcaacaaacgcgcaggcggaatggggctgattcttgga
    cgtaaagcgttcaagaaatcgatggctgacggcgtgaaactgattaacgccgtgcaggac
    gtttatctcgatagcaaaattactatcgcctga
    > Translation of ORF number 1 in reading frame 1 on the direct strand.
    MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNNRPPAVLRNMQTLYNTG
    RLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAIEAGCNCVASTYGVLASVS
    RRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMGAVAVGATIYFGSEESRRQIE
    EISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSADLTGQANHLAATIGADIVKQKM
    AENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQLANCYMGRAGLINSGGAAGGETDLS
    DAVRTAVINKRAGGMGLILGRKAFKKSMADGVKLINAVQDVYLDSKITIA*
    6) PCR Primer Stats
    PCR Primer Stats results
    Global settings:
    -The primers do not have a 5'-phosphate group.
    -Combined concentration of K+ and Na+ in the reaction = 50 millimolar.
    -Mg+2 concentration in the reaction = 1.5 millimolar.
    -Primer concentration in the reaction = 200 nanomolar.
    7) Restriction Digest
    Restriction Digest results
    >326 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 248 to base 573 (AluI ag|ct - AluI ag|ct).
    cttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactggcgatcg
    aagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcgcggcgtt
    atgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagttacccga
    atacctacgatcaaacgctgtatgccagcgtggagcaggcgttcaacatgggcgcggttg
    cggttggtgcgactatctattttggctcggaagagtcacgtcgccagattgaagaaattt
    ctgcggcttttgaacgtgcgcacgag
    >256 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 574 to base 829 (AluI ag|ct - AluI ag|ct).
    ctgggtatggtgacagtgctgtgggcctatttgcgtaactccgcctttaagaaagatggc
    gttgattaccatgtttccgccgacctgaccggtcaggcaaaccatctggcggcaaccatc
    ggtgcagatatcgtcaaacaaaaaatggcggaaaataacggcggctataaagcaattaat
    tacggttacaccgacgatcgtgtttacagcaaattgaccagcgaaaacccgattgatctg
    gtgcgttatcagttag
    >224 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 830 to base 1053 (AluI ag|ct - sequence end).
    ctaactgctatatgggtcgggctgggttgataaactccggcggtgctgcgggcggtgaaa
    ctgacctcagcgatgcagtgcgtactgcggttatcaacaaacgcgcaggcggaatggggc
    tgattcttggacgtaaagcgttcaagaaatcgatggctgacggcgtgaaactgattaacg
    ccgtgcaggacgtttatctcgatagcaaaattactatcgcctga
    >166 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 82 to base 247 (AluI ag|ct - AluI ag|ct).
    ctttatctccccggacatgactacgtagaccgcgtaatgattgacaataatcgcccgcca
    gcggtgttacgtaatatgcagacgttgtacaacaccgggcgtctggctggcacaggatat
    ctttctattctgccggttgaccagggcgttgagcactctgccggag
    >81 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 1 to base 81 (sequence start - AluI ag|ct).
    atgacagatattgcgcagttgcttggcaaagacgccgacaaccttttacagcaccgttgt
    atgacaattccttctgaccag
    8) Restriction Summary
    Restriction Summary results
    doesn't cut
    cuts once
    cuts twice
    cuts three times
    Results for linear 1053 residue sequence "eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)" starting "atgacagata"
    Site:
    Positions :
    AatI agg|cct
    none
    AatII gacgt|c
    none
    Acc16I tgc|gca
    15, 372, 567
    AccII cg|cg
    114, 361, 482, 933
    AccIII t|ccgga
    none
    AclI aa| cgtt
    none
    AcvI cac|gtg
    none
    AfaI gt|ac
    170, 913
    AfeI agc|gct
    none
    AflII c|ttaag
    none
    AgeI a|ccggt
    662
    AhlI a|ctagt
    none
    Alw441 g|tgcac
    none
    AluI ag|ct
    82, 248, 574, 830
    Aor51HI agc|gct
    none
    ApaI gggcc|c
    none
    ApaLI g|tgcac
    none
    AscI gg|cgcgcc
    none
    AseI at|taat
    750
    Asp718I g|gtacc
    none
    AsuII tt| cgaa
    none
    AvaI c|ycgrg
    none
    AviII tgc|gca
    15, 372, 567
    AvrII c|ctagg
    none
    BalI tgg|cca
    none
    BamHI g|gatcc
    none
    BanIII at| cgat
    980
    BbeI ggcgc|c
    none
    BbrPI cac|gtg
    none
    BbuI gcatg|c
    none
    BcuI a|ctagt
    none
    BclI t|gatca
    none
    BfaI c|tag
    none
    BfrI c|ttaag
    none
    BfrBI atg|cat
    none
    BglII a|gatct
    none
    BlnI c|ctagg
    none
    BseCI at|cgat
    980
    BsePI g|cgcgc
    none
    BseX3I c| ggccg
    none
    BshTI a|ccggt
    662
    Bsp1407I t|gtaca
    168
    Bsp19I c|catgg
    none
    BspDI at|cgat
    980
    BspEI t|ccgga
    none
    BsrGI t|gtaca
    168
    BssHII g|cgcgc
    none
    BstUI cg|cg
    114, 361, 482, 933
    ClaI at|cgat
    980
    DraI ttt|aaa
    none
    EagI c|ggccg
    none
    EcoRI g|aattc
    none
    EcoRV gat|atc
    200, 703
    EgeI ggc|gcc
    none
    FseI ggccgg|cc
    none
    FspI tgc|gca
    15, 372, 567
    HaeIII gg|cc
    599
    HincII gty|rac
    220, 333
    HindIII a|agctt
    none
    HinfI g|antc
    521, 952
    HpaI gtt|aac
    none
    HpaII c|cgg
    93, 177, 215, 243, 663, 867
    KasI g|gcgcc
    none
    KpnI ggtac|c
    none
    MboI |gatc
    303, 436, 769, 808
    MfeI c|aattg
    none
    MluI a| cgcgt
    none
    MscI tgg|cca
    none
    MseI t|taa
    399, 621, 750, 1005
    MspI c|cgg
    93, 177, 215, 243, 663, 867
    NaeI gcc|ggc
    none
    NarI gg| cgcc
    none
    NcoI c|catgg
    none
    NdeI ca|tatg
    none
    NdeII |gatc
    303, 436, 769, 808
    NgoMIV g|ccggc
    none
    NheI g|ctagc
    none
    NotI gc|ggccgc
    none
    NruI tcg|cga
    none
    NsiI atgca|t
    none
    PacI ttaat|taa
    none
    PciI a|catgt
    none
    PhoI gg|cc
    599
    PmeI gttt|aaac
    none
    PmlI cac|gtg
    none
    PsiI tta|taa
    none
    PstI ctgca|g
    none
    PvuI cgat|cg
    306, 772
    PvuII cag|ctg
    none
    RsaI gt|ac
    170, 913
    SacI gagct|c
    none
    SacII ccgc|gg
    none
    SalI g|tcgac
    none
    SbfI cctgca|gg
    none
    ScaI agt|act
    none
    SfoI ggc|gcc
    none
    SmaI ccc|ggg
    none
    SnaBI tac|gta
    106, 152
    SpeI a|ctagt
    none
    SphI gcatg|c
    none
    SspI aat|att
    none
    SstI gagct|c
    none
    SstII ccgc|gg
    none
    StuI agg|cct
    none
    SwaI attt|aaat
    none
    TaqI t|cga
    306, 980, 1029
    TliI c|tcgag
    none
    VspI at|taat
    750
    XbaI t|ctaga
    none
    XhoI c|tcgag
    none
    XmaI c|ccggg
    none
    9) Translate
    Translate results
    >rf 1 eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)
    MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNNRPPAVLRNMQTLYNTG
    RLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAIEAGCNCVASTYGVLASVS
    RRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMGAVAVGATIYFGSEESRRQIE
    EISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSADLTGQANHLAATIGADIVKQKM
    AENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQLANCYMGRAGLINSGGAAGGETDLS
    DAVRTAVINKRAGGMGLILGRKAFKKSMADGVKLINAVQDVYLDSKITIA*
    10) Group DNA
    Group DNA results
    Results for 1053 residue sequence "eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N)" starting "atgacagata"
    1 atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt
    61 atgacaattc cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg
    121 attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg
    181 cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct
    241 gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg
    301 gcgatcgaag cgggctgtaa ctgtgtggcg tcaacttacg gcgtgctggc gtcggtatcg
    361 cggcgttatg cgcatcgcat tccattcctc gtcaaactta atcacaacga gacgctaagt
    421 tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcgtt caacatgggc
    481 gcggttgcgg ttggtgcgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa
    541 gaaatttctg cggcttttga acgtgcgcac gagctgggta tggtgacagt gctgtgggcc
    601 tatttgcgta actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg
    661 accggtcagg caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg
    721 gcggaaaata acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttac
    781 agcaaattga ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat
    841 atgggtcggg ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagc
    901 gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg gaatggggct gattcttgga
    961 cgtaaagcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac
    1021 gtttatctcg atagcaaaat tactatcgcc tga
    3. SPdbV ( http://www.expasy.org/spdbv/ ) kasutamine.
    a. Leida vastavate valkude ruumiliste struktuuride PDB failid (alternatiiv http://www.rcsb.org/pdb/ ) ja laadida need arvutisse ning kirjeldada neid (peaahela käik, sekundaarstruktuuride olemasolu ja ulatus, elektrostaatilised ja molekulaarsed pinnad).
    Peaahela käik
    Sekundaarstruktuurid
    Elektrostaatilised pinnad
    Molekulaarsed pinnad
    4. Andmete otsimine NCBI-st Entrezi kaudu ( http://www.ncbi.nih.gov/ )
    a.Leida Drosophila melanogaster püruvaatkinaasi kodeeriv geen NCBI RefSeq-s (geeni tüüp, kirjeldus, nimi, funktsioon, protsessid), töötada MapVieweriga.
    Gene name
    CG7069
    Gene description
    CG7069
    Gene type
    protein coding
    Function Evidence
    carbohydrate kinase activity IEA
    pyruvate kinase activity ISS
    Process Evidence
    glycolysis IEA
    Drosophila melanogaster püruvaatkinaasi kodeeriva geeni lehekülg:
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=full_report&list_uids=42621#refseq
    b.Leida vastav mRNA järjestus ja moodustada GenBanki, FASTA ja graafiline formaat .
    Vastav mRNA järjestus:
    FASTA formaat:
    >gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    ATTTTATTCGTTTTTGACAGGTAACACTACATTTTATTTTTGTTTCGGTGTATCAAAATTTATTCAAAAT
    TCATCCATACGCAAGAATCCTTAGTCCAGGGTCCACAGTGCCCATATGTTCCACTTGCCTGTTAAACCTA
    AGATATCGCTGCTATGCCAGCAAATGCAAGAAAGAGAAGAAACCACCGAAGTTCCGACTGGACCGCGGAC
    CATTCGCCTCCCAGTTGGATTACCAGTCGCGACTGCAGTACCAAGCACCTGCCCTGAGTATCCCCCTCAG
    CAGCATCATATGCACCATTGGACCCTCATCCAACAGCCCAGAAAAGCTATTGGAGCTCATCCGGGCTGGG
    ATGCGGGTCGTGCGTATGAACTTCTCTCACGGCTCTCACGAGTATCACTGCCAGACAATACAGGCGGCTC
    GTAAGGCCATCGCCATGTACGTGGAGCAAACGGGTCTGCCCAGAACATTGGCAATTGCTCTGGACACCAA
    GGGTCCAGAGATCCGGACGGGCAAACTGGCCGGTGGCAATGATAGGGCTGAGATAGAGCTCAAGACCGGA
    GACAAAGTGACATTGAGCACCAAGAAGGAGATGGCAGACAAGAGTAACAAGGATAATATCTATGTGGATT
    ATCAGAGGCTACCTCAACTGGTCAAACCAGGAAATCGGGTATTTGTAGACGACGGATTGATTGCATTGAT
    CGTGAAGGAATCAAAGGGAGATGAAGTGATCTGTCAGGTGGAGAACGGTGGCAAGCTGGGCTCCCACAAG
    GGAATCAATTTGCCAGGAGTTCCGGTAGATCTTCCCAGCGTAACGGAGAAGGATAAGCAGGATCTTAAAT
    TTGGGGCCGAGCAAAAGGTGGACATGATCTTCGCATCGTTCATACGAGACGCAAATGCTCTGAAGGAAAT
    CCGCCAAGTACTTGGACCGGCTGGAGCGTGTATAAAGATCATCTCTAAGATTGAAAACCACCAAGGTCTG
    GTAAACATAGACGACATTATCCGTGAATCAGATGGCATAATGGTGGCCCGAGGTGATATGGGCATTGAGA
    TACCCACGGAGGATGTTCCCCTAGCCCAGAAATCGATTGTGGCTAAGTGCAACAAGGTGGGCAAGCCTGT
    GATCTGTGCCACCCAGATGATGGAGTCGATGACGAACAAGCCGCGACCCACTCGTGCCGAAGCCTCGGAT
    GTGGCCAACGCTATTTTCGATGGCTGCGATGCCGTAATGCTGTCCGGTGAAACGGCCAAGGGCAAGTATC
    CGGTGGAGTGCGTCCAGTGCATGGCCAGGATCTGTGCCAAAGTAGAAGCAGTTCTATGGTACGAAAGCCT
    GCAGAACAGCCTTAAAAGGGAAATCCGGACCAGTGCTGCCGACCACATTTCGGCAGTTACCACGGCGATC
    GCGGAGGCGGCCACGGTGGGCCAAGCCCGGGCAATTGTGGTGGCCAGTCCGTGCTCCATGGTGGCCCAAA
    TGGTCAGTCACATGAGGCCGCCATGTCCAATTGTCATGCTCACGGGTAACGAATCCGAGGCGGCCCAGTC
    TCTGCTCTTTCGCGGCATCTACCCACTCCTCGTTGAGGAAATGGTTATTGGCAGCTTTAACTTTCGACGC
    ATCATGCAGTCGGGCCTGAAGCTGATGGGCAAGATGGACATCTTGGAGCCGGGTCAGAAAGGATCGGTGG
    TGCTGGTTAATGCCATGTCGGCAGAAAAGATCACCTTCCGGCTGTTCACTATCCGTCAGCAGACCAAGGA
    AGAACGCGATCAGGATGAGCGATGCCGAAAATTGGCCCTAGAGCAGAGCTGCAAGGAGAGGGCTGAAAAG
    GAGGAATGCAGAAAGCTCCAGCAAGCGGAAGAATGTCAGAAGCAAAAACTGGCCAAAAAATGTAAACAGT
    TCGAAGAGAAGCAAAAGGTCTGCCCCAAAAAGAATGATACTCCGAAGAATGATTGTCCGAAGAAAGATTG
    TCCGAAGAAAGAATGTCCCAAGCAAGATGACGAAATCTCAAAGTGCAGGCAAATGCAAGAAGCGGAAGCT
    GAAGAAAGGAAGTGCAAAGAAGAGTTTGAACAGATGTGCAAATTAGCTGAAGAAAAAAGGAAAGAAGCTG
    AAAAATGCAGGAAAGCGGACGAAGAACGAAGGAAAGAGGAAGCTGAAAAGTGCAGGAAATTGGAAGAAGA
    TAGGAAATGCAAATTGGCTGAAGAGAAGAAAAGGAATGAAGAAGAACTTAAGATAATAGAAGCAGAAGTG
    GCCAAACTGGAAGCTGCTGAGAAGGCCAAGCGTTTGAAGGAGGAAGAGAAAAAGAAGGAAGAACTAATGA
    AGTGCAAGCAACGAAATGAAGCGAAAAAAAAGAGGGAAGAGGCTGAAAGGTGCAAGAGAAAGGAAAGAGA
    AAGGGAGCTAGCTGAAATGGAAAACAAATGGAAACAGGTTGCGGAGAAAAGAAAGAGAAAGAAGGCAGCA
    GAGATGTGCAGGAAAATAGAGGATGCAAAAGAAAAAGCCGCGGCAGAATCGGCTGATAAGATTCTGAAAG
    CTGTGTGCGAAAAACTAAAGCAATCTCTCTCGGATCCGGACAAATCAAAAAAGGGCAAGAAATAAAGTGA
    AAGCTAATGGCAGATTTATATGGAACATTTTAACTTTTAATACGGTGTTACACACCGAATTCCAAATTAT
    TATATAAAACTATTGGAATTTTTTAAATTTTCT
    GenBank formaat:
    LOCUS NM_142773 2693 bp mRNA linear INV 12-OCT-2006
    DEFINITION Drosophila melanogaster CG7069-RA (CG7069), mRNA.
    ACCESSION NM_142773
    VERSION NM_142773.1 GI:24648965
    KEYWORDS .
    SOURCE Drosophila melanogaster (fruit fly)
    ORGANISM Drosophila melanogaster
    Eukaryota; Metazoa ; Arthropoda; Hexapoda; Insecta; Pterygota;
    Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
    Ephydroidea; Drosophilidae; Drosophila.
    REFERENCE 1 (bases 1 to 2693)
    AUTHORS Quesneville,H., Bergman ,C.M., Andrieu,O., Autard,D., Nouaud,D.,
    Ashburner,M. and Anxolabehere,D.
    TITLE Combined evidence annotation of transposable elements in genome
    sequences
    JOURNAL PLoS Comput. Biol. 1 (2), e22 (2005)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/16110336?dopt=Abstract
    REFERENCE 2 (bases 1 to 2693)
    AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., de Carvalho,A.B.,
    Halpern,A., Kaminker,J.S., Kennedy ,C., Mungall,C.J., Sullivan ,B.A.,
    Sutton ,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers ,E.W.,
    Celniker,S.E., Rubin ,G.M. and Karpen,G.H.
    TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun
    assembly
    JOURNAL Genome Biol. 3 (12), RESEARCH0085 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537574?dopt=Abstract
    REFERENCE 3 (bases 1 to 2693)
    AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J.,
    Svirskas,R., Patel ,S., Frise,E., Wheeler,D.A., Lewis ,S.E.,
    Rubin,G.M., Ashburner,M. and Celniker,S.E.
    TITLE The transposable elements of the Drosophila melanogaster
    euchromatin: a genomics perspective
    JOURNAL Genome Biol. 3 (12), RESEARCH0084 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537573?dopt=Abstract
    REFERENCE 4 (bases 1 to 2693)
    AUTHORS Misra,S., Crosby ,M.A., Mungall,C.J., Matthews,B.B., Campbell ,K.S.,
    Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E.,
    Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L.,
    Berman ,B.P., Bettencourt,B.R., Celniker,S.E., de Grey ,A.D.,
    Drysdale,R.A., Harris ,N.L., Richter ,J., Russo ,S., Schroeder,A.J.,
    Shu,S.Q., Stapleton ,M., Yamada,C., Ashburner,M., Gelbart,W.M.,
    Rubin,G.M. and Lewis,S.E.
    TITLE Annotation of the Drosophila melanogaster euchromatic genome: a
    systematic review
    JOURNAL Genome Biol. 3 (12), RESEARCH0083 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537572?dopt=Abstract
    REFERENCE 5 (bases 1 to 2693)
    AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W.,
    Halpern,A., Patel,S., Adams ,M., Champe,M., Dugan,S.P., Frise,E.,
    Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M.,
    Nelson ,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards ,S.,
    Sodergren,E.J., Svirskas,R., Tabor ,P.E., Wan,K., Stapleton,M.,
    Sutton,G.G., Venter ,C., Weinstock,G., Scherer,S.E., Myers,E.W.,
    Gibbs ,R.A. and Rubin,G.M.
    TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila
    melanogaster euchromatic genome sequence
    JOURNAL Genome Biol. 3 (12), RESEARCH0079 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537568?dopt=Abstract
    REFERENCE 6 (bases 1 to 2693)
    AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans ,C.A., Gocayne,J.D.,
    Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle ,R.F.,
    George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson ,S.N.,
    Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang ,Q., Chen ,L.X.,
    Brandon ,R.C., Rogers ,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D.,
    Wan,K.H., Doyle ,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L.,
    Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D.,
    Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M.,
    Beeson,K.Y., Benos ,P.V., Berman,B.P., Bhandari,D., Bolshakov,S.,
    Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P.,
    Burtis,K.C., Busam,D.A., Butler ,H., Cadieu,E., Center,A.,
    Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B.,
    Davies,P., de Pablos,B., Delcher,A., Deng ,Z., Mays,A.D., Dew,I.,
    Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S.,
    Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C.,
    Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S.,
    Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z.,
    Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J.,
    Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J.,
    Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z.,
    Kennison,J.A., Ketchum,K.A., Kimmel ,B.E., Kodira,C.D., Kraft ,C.,
    Kravitz,S., Kulp ,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A.,
    Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C.,
    McLeod ,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C.,
    Morris ,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy ,B., Murphy,L.,
    Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon ,K.,
    Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S.,
    Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K.,
    Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I.,
    Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C.,
    Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner ,R.,
    Venter,E., Wang ,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A.,
    Weinstock,G.M., Weissenbach,J., Williams ,S.M., WoodageT,
    Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F.,
    Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H.,
    Zhong,F.N., Zhong,W., Zhou ,X., Zhu,S., Zhu,X., Smith,H.O.,
    Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C.
    TITLE The genome sequence of Drosophila melanogaster
    JOURNAL Science 287 (5461), 2185-2195 (2000)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/10731132?dopt=Abstract
    REFERENCE 7 (bases 1 to 2693)
    AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R.,
    Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R.,
    Smith,E., Yu,C. and Rubin,G.
    CONSRTM Berkeley Drosophila Genome Project
    TITLE Drosophila melanogaster release 4 sequence
    JOURNAL Unpublished
    REFERENCE 8 (bases 1 to 2693)
    AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R.,
    Park,S., Svirskas,R. and Karpen,G.
    TITLE Direct Submission
    JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project,
    Lawrence Berkeley National Laboratory , One Cyclotron Road, MS
    64-121, Berkeley, CA 94720, USA
    REFERENCE 9 (bases 1 to 2693)
    AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S.,
    Svirskas,R. and Rubin,G.
    TITLE Direct Submission
    JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project,
    Lawrence Berkeley National Laboratory, One Cyclotron Road, MS
    64-121, Berkeley, CA 94720, USA
    REFERENCE 10 (bases 1 to 2693)
    AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J.
    TITLE Direct Submission
    JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive,
    Rockville, MD 20850, USA
    COMMENT REVIEWED REFSEQ: This record has been curated by FlyBase. This
    record is derived from an annotated genomic sequence (NT_033777).
    The reference sequence was derived from CG7069-RA.
    FEATURES Location /Qualifiers
    source 1..2693
    /organism="Drosophila melanogaster"
    /mol_type="mRNA"
    /db_xref=" taxon :7227"
    /chromosome="3R"
    gene 1..2693
    /gene="CG7069"
    /locus_tag="Dmel_CG7069"
    /old_locus_tag="CG7069"
    /note="CG7069"
    /map="94A6-94A6"
    /db_xref="FLYBASE:FBgn0038952"
    /db_xref="GeneID:42621"
    CDS 351..2585
    /gene="CG7069"
    /locus_tag="Dmel_CG7069"
    /old_locus_tag="CG7069"
    /note="CG7069 gene product from transcript CG7069-RA"
    /codon_start=1
    /product="CG7069-PA"
    /protein_id="NP_651030.1"
    /db_xref="GI:24648966"
    /db_xref="FLYBASE:FBgn0038952"
    /db_xref="GeneID:42621"
    /translation="MRVVRMNFSHGSHEYHCQTIQAARKAIAMYVEQTGLPRTLAIAL
    DTKGPEIRTGKLAGGNDRAEIELKTGDKVTLSTKKEMADKSNKDNIYVDYQRLPQLVK
    PGNRVFVDDGLIALIVKESKGDEVICQVENGGKLGSHKGINLPGVPVDLPSVTEKDKQ
    DLKFGAEQKVDMIFASFIRDANALKEIRQVLGPAGACIKIISKIENHQGLVNIDDIIR
    ESDGIMVARGDMGIEIPTEDVPLAQKSIVAKCNKVGKPVICATQMMESMTNKPRPTRA
    EASDVANAIFDGCDAVMLSGETAKGKYPVECVQCMARICAKVEAVLWYESLQNSLKRE
    IRTSAADHISAVTTAIAEAATVGQARAIVVASPCSMVAQMVSHMRPPCPIVMLTGNES
    EAAQSLLFRGIYPLLVEEMVIGSFNFRRIMQSGLKLMGKMDILEPGQKGSVVLVNAMS
    AEKITFRLFTIRQQTKEERDQDERCRKLALEQSCKERAEKEECRKLQQAEECQKQKLA
    KKCKQFEEKQKVCPKKNDTPKNDCPKKDCPKKECPKQDDEISKCRQMQEAEAEERKCK
    EEFEQMCKLAEEKRKEAEKCRKADEERRKEEAEKCRKLEEDRKCKLAEEKKRNEEELK
    IIEAEVAKLEAAEKAKRLKEEEKKKEELMKCKQRNEAKKKREEAERCKRKERERELAE
    MENKWKQVAEKRKRKKAAEMCRKIEDAKEKAAAESADKILKAVCEKLKQSLSDPDKSK
    KGKK"
    ORIGIN
    1 attttattcg tttttgacag gtaacactac attttatttt tgtttcggtg tatcaaaatt
    61 tattcaaaat tcatccatac gcaagaatcc ttagtccagg gtccacagtg cccatatgtt
    121 ccacttgcct gttaaaccta agatatcgct gctatgccag caaatgcaag aaagagaaga
    181 aaccaccgaa gttccgactg gaccgcggac cattcgcctc ccagttggat taccagtcgc
    241 gactgcagta ccaagcacct gccctgagta tccccctcag cagcatcata tgcaccattg
    301 gaccctcatc caacagccca gaaaagctat tggagctcat ccgggctggg atgcgggtcg
    361 tgcgtatgaa cttctctcac ggctctcacg agtatcactg ccagacaata caggcggctc
    421 gtaaggccat cgccatgtac gtggagcaaa cgggtctgcc cagaacattg gcaattgctc
    481 tggacaccaa gggtccagag atccggacgg gcaaactggc cggtggcaat gatagggctg
    541 agatagagct caagaccgga gacaaagtga cattgagcac caagaaggag atggcagaca
    601 agagtaacaa ggataatatc tatgtggatt atcagaggct acctcaactg gtcaaaccag
    661 gaaatcgggt atttgtagac gacggattga ttgcattgat cgtgaaggaa tcaaagggag
    721 atgaagtgat ctgtcaggtg gagaacggtg gcaagctggg ctcccacaag ggaatcaatt
    781 tgccaggagt tccggtagat cttcccagcg taacggagaa ggataagcag gatcttaaat
    841 ttggggccga gcaaaaggtg gacatgatct tcgcatcgtt catacgagac gcaaatgctc
    901 tgaaggaaat ccgccaagta cttggaccgg ctggagcgtg tataaagatc atctctaaga
    961 ttgaaaacca ccaaggtctg gtaaacatag acgacattat ccgtgaatca gatggcataa
    1021 tggtggcccg aggtgatatg ggcattgaga tacccacgga ggatgttccc ctagcccaga
    1081 aatcgattgt ggctaagtgc aacaaggtgg gcaagcctgt gatctgtgcc acccagatga
    1141 tggagtcgat gacgaacaag ccgcgaccca ctcgtgccga agcctcggat gtggccaacg
    1201 ctattttcga tggctgcgat gccgtaatgc tgtccggtga aacggccaag ggcaagtatc
    1261 cggtggagtg cgtccagtgc atggccagga tctgtgccaa agtagaagca gttctatggt
    1321 acgaaagcct gcagaacagc cttaaaaggg aaatccggac cagtgctgcc gaccacattt
    1381 cggcagttac cacggcgatc gcggaggcgg ccacggtggg ccaagcccgg gcaattgtgg
    1441 tggccagtcc gtgctccatg gtggcccaaa tggtcagtca catgaggccg ccatgtccaa
    1501 ttgtcatgct cacgggtaac gaatccgagg cggcccagtc tctgctcttt cgcggcatct
    1561 acccactcct cgttgaggaa atggttattg gcagctttaa ctttcgacgc atcatgcagt
    1621 cgggcctgaa gctgatgggc aagatggaca tcttggagcc gggtcagaaa ggatcggtgg
    1681 tgctggttaa tgccatgtcg gcagaaaaga tcaccttccg gctgttcact atccgtcagc
    1741 agaccaagga agaacgcgat caggatgagc gatgccgaaa attggcccta gagcagagct
    1801 gcaaggagag ggctgaaaag gaggaatgca gaaagctcca gcaagcggaa gaatgtcaga
    1861 agcaaaaact ggccaaaaaa tgtaaacagt tcgaagagaa gcaaaaggtc tgccccaaaa
    1921 agaatgatac tccgaagaat gattgtccga agaaagattg tccgaagaaa gaatgtccca
    1981 agcaagatga cgaaatctca aagtgcaggc aaatgcaaga agcggaagct gaagaaagga
    2041 agtgcaaaga agagtttgaa cagatgtgca aattagctga agaaaaaagg aaagaagctg
    2101 aaaaatgcag gaaagcggac gaagaacgaa ggaaagagga agctgaaaag tgcaggaaat
    2161 tggaagaaga taggaaatgc aaattggctg aagagaagaa aaggaatgaa gaagaactta
    2221 agataataga agcagaagtg gccaaactgg aagctgctga gaaggccaag cgtttgaagg
    2281 aggaagagaa aaagaaggaa gaactaatga agtgcaagca acgaaatgaa gcgaaaaaaa
    2341 agagggaaga ggctgaaagg tgcaagagaa aggaaagaga aagggagcta gctgaaatgg
    2401 aaaacaaatg gaaacaggtt gcggagaaaa gaaagagaaa gaaggcagca gagatgtgca
    2461 ggaaaataga ggatgcaaaa gaaaaagccg cggcagaatc ggctgataag attctgaaag
    2521 ctgtgtgcga aaaactaaag caatctctct cggatccgga caaatcaaaa aagggcaaga
    2581 aataaagtga aagctaatgg cagatttata tggaacattt taacttttaa tacggtgtta
    2641 cacaccgaat tccaaattat tatataaaac tattggaatt ttttaaattt tct
    Graafiline formaat:
    http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&qty=1&c_start=1&list_uids=NM_142773.1&uids=&dopt=graph&dispmax=5&sendto=&fmt_mask=0&from=begin&to=end&extrafeatpresent=1&ef_CDD=8&ef_MGC=16&ef_HPRD=32&ef_STS=64&ef_tRNA=128&ef_microRNA=256
    c .Leida järjestusega seotud haigused ja kirjeldada neid lühidalt (OMIM).
    Antud järjestusega seotud haiguste kohta informatsioon puudub.
  • Leida vastava geeniga seotud valk ja moodustada GenPept, FASTA ja graafiline formaat.
    Vastava geeniga seotud valgu:
    FASTA formaat:
    >gi|24648966|ref|NP_651030.1| CG7069-PA [Drosophila melanogaster]
    MRVVRMNFSHGSHEYHCQTIQAARKAIAMYVEQTGLPRTLAIALDTKGPEIRTGKLAGGNDRAEIELKTG
    DKVTLSTKKEMADKSNKDNIYVDYQRLPQLVKPGNRVFVDDGLIALIVKESKGDEVICQVENGGKLGSHK
    GINLPGVPVDLPSVTEKDKQDLKFGAEQKVDMIFASFIRDANALKEIRQVLGPAGACIKIISKIENHQGL
    VNIDDIIRESDGIMVARGDMGIEIPTEDVPLAQKSIVAKCNKVGKPVICATQMMESMTNKPRPTRAEASD
    VANAIFDGCDAVMLSGETAKGKYPVECVQCMARICAKVEAVLWYESLQNSLKREIRTSAADHISAVTTAI
    AEAATVGQARAIVVASPCSMVAQMVSHMRPPCPIVMLTGNESEAAQSLLFRGIYPLLVEEMVIGSFNFRR
    IMQSGLKLMGKMDILEPGQKGSVVLVNAMSAEKITFRLFTIRQQTKEERDQDERCRKLALEQSCKERAEK
    EECRKLQQAEECQKQKLAKKCKQFEEKQKVCPKKNDTPKNDCPKKDCPKKECPKQDDEISKCRQMQEAEA
    EERKCKEEFEQMCKLAEEKRKEAEKCRKADEERRKEEAEKCRKLEEDRKCKLAEEKKRNEEELKIIEAEV
    AKLEAAEKAKRLKEEEKKKEELMKCKQRNEAKKKREEAERCKRKERERELAEMENKWKQVAEKRKRKKAA
    EMCRKIEDAKEKAAAESADKILKAVCEKLKQSLSDPDKSKKGKK
    GenPept formaat:
    LOCUS NP_651030 744 aa linear INV 12-OCT-2006
    DEFINITION CG7069-PA [Drosophila melanogaster].
    ACCESSION NP_651030
    VERSION NP_651030.1 GI:24648966
    DBSOURCE REFSEQ: accession NM_142773.1
    KEYWORDS .
    SOURCE Drosophila melanogaster (fruit fly)
    ORGANISM Drosophila melanogaster
    Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
    Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
    Ephydroidea; Drosophilidae; Drosophila.
    REFERENCE 1 (residues 1 to 744)
    AUTHORS Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D.,
    Ashburner,M. and Anxolabehere,D.
    TITLE Combined evidence annotation of transposable elements in genome
    sequences
    JOURNAL PLoS Comput. Biol. 1 (2), e22 (2005)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/16110336?dopt=Abstract
    REFERENCE 2 (residues 1 to 744)
    AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., de Carvalho,A.B.,
    Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A.,
    Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W.,
    Celniker,S.E., Rubin,G.M. and Karpen,G.H.
    TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun
    assembly
    JOURNAL Genome Biol. 3 (12), RESEARCH0085 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537574?dopt=Abstract
    REFERENCE 3 (residues 1 to 744)
    AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J.,
    Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E.,
    Rubin,G.M., Ashburner,M. and Celniker,S.E.
    TITLE The transposable elements of the Drosophila melanogaster
    euchromatin: a genomics perspective
    JOURNAL Genome Biol. 3 (12), RESEARCH0084 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537573?dopt=Abstract
    REFERENCE 4 (residues 1 to 744)
    AUTHORS Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S.,
    Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E.,
    Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L.,
    Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D.,
    Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J.,
    Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M.,
    Rubin,G.M. and Lewis,S.E.
    TITLE Annotation of the Drosophila melanogaster euchromatic genome: a
    systematic review
    JOURNAL Genome Biol. 3 (12), RESEARCH0083 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537572?dopt=Abstract
    REFERENCE 5 (residues 1 to 744)
    AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W.,
    Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E.,
    Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M.,
    Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S.,
    Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M.,
    Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W.,
    Gibbs,R.A. and Rubin,G.M.
    TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila
    melanogaster euchromatic genome sequence
    JOURNAL Genome Biol. 3 (12), RESEARCH0079 (2002)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/12537568?dopt=Abstract
    REFERENCE 6 (residues 1 to 744)
    AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D.,
    Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F.,
    George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N.,
    Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X.,
    Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D.,
    Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L.,
    Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D.,
    Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M.,
    Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S.,
    Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P.,
    Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A.,
    Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B.,
    Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I.,
    Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S.,
    Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C.,
    Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S.,
    Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z.,
    Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J.,
    Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J.,
    Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z.,
    Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C.,
    Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A.,
    Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C.,
    McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C.,
    Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L.,
    Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K.,
    Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S.,
    Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K.,
    Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I.,
    Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C.,
    Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R.,
    Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A.,
    Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT,
    Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F.,
    Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H.,
    Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O.,
    Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C.
    TITLE The genome sequence of Drosophila melanogaster
    JOURNAL Science 287 (5461), 2185-2195 (2000)
    PUBMED http://www.ncbi.nlm.nih.gov/pubmed/10731132?dopt=Abstract
    REFERENCE 7 (residues 1 to 744)
    AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R.,
    Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R.,
    Smith,E., Yu,C. and Rubin,G.
    CONSRTM Berkeley Drosophila Genome Project
    TITLE Drosophila melanogaster release 4 sequence
    JOURNAL Unpublished
    REFERENCE 8 (residues 1 to 744)
    AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R.,
    Park,S., Svirskas,R. and Karpen,G.
    TITLE Direct Submission
    JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project,
    Lawrence Berkeley National Laboratory, One Cyclotron Road, MS
    64-121, Berkeley, CA 94720, USA
    REFERENCE 9 (residues 1 to 744)
    AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S.,
    Svirskas,R. and Rubin,G.
    TITLE Direct Submission
    JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project,
    Lawrence Berkeley National Laboratory, One Cyclotron Road, MS
    64-121, Berkeley, CA 94720, USA
    REFERENCE 10 (residues 1 to 744)
    AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J.
    TITLE Direct Submission
    JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive,
    Rockville, MD 20850, USA
    COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
    reference sequence was derived from AAF55980.
    Method : conceptual translation.
    FEATURES Location/Qualifiers
    source 1..744
    /organism="Drosophila melanogaster"
    /db_xref="taxon:7227"
    /chromosome="3R"
    Protein 1..744
    /product="CG7069-PA"
    /name="CG7069 gene product from transcript CG7069-RA"
    /calculated_mol_wt=83867
    Region 1..410
    /region_name="Pyruvate_Kinase"
    /note="Pyruvate kinase (PK): Large allosteric enzyme that
    regulates glycolysis through binding of the substrate,
    phosphoenolpyruvate, and one or more allosteric effectors;
    cd00288"
    /db_xref="CDD:29370"
    CDS 1..744
    /gene="CG7069"
    /locus_tag="Dmel_CG7069"
    /old_locus_tag="CG7069"
    /coded_by="NM_142773.1:351..2585"
    /db_xref="FLYBASE:FBgn0038952"
    /db_xref="GeneID:42621"
    ORIGIN
    1 mrvvrmnfsh gsheyhcqti qaarkaiamy veqtglprtl aialdtkgpe irtgklaggn
    61 draeielktg dkvtlstkke madksnkdni yvdyqrlpql vkpgnrvfvd dglialivke
    121 skgdevicqv enggklgshk ginlpgvpvd lpsvtekdkq dlkfgaeqkv dmifasfird
    181 analkeirqv lgpagaciki iskienhqgl vniddiires dgimvargdm gieiptedvp
    241 laqksivakc nkvgkpvica tqmmesmtnk prptraeasd vanaifdgcd avmlsgetak
    301 gkypvecvqc maricakvea vlwyeslqns lkreirtsaa dhisavttai aeaatvgqar
    361 aivvaspcsm vaqmvshmrp pcpivmltgn eseaaqsllf rgiypllvee mvigsfnfrr
    421 imqsglklmg kmdilepgqk gsvvlvnams aekitfrlft irqqtkeerd qdercrklal
    481 eqsckeraek eecrklqqae ecqkqklakk ckqfeekqkv cpkkndtpkn dcpkkdcpkk
    541 ecpkqddeis kcrqmqeaea eerkckeefe qmcklaeekr keaekcrkad eerrkeeaek
    601 crkleedrkc klaeekkrne eelkiieaev akleaaekak rlkeeekkke elmkckqrne
    661 akkkreeaer ckrkererel aemenkwkqv aekrkrkkaa emcrkiedak ekaaaesadk
    721 ilkavceklk qslsdpdksk kgkk
    Graafiline formaat:
    http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&qty=1&c_start=1&list_uids=NP_651030.1&uids=&dopt=graph&dispmax=5&sendto=&from=begin&to=end&extrafeatpresent=1&ef_CDD=8&ef_MGC=16&ef_HPRD=32&ef_STS=64&ef_tRNA=128&ef_microRNA=256
    5. BioEdit ( http://www.mbio.ncsu.edu/BioEdit/page2.html ) kasutamine.
    a. Installeerida arvutisse BioEdit, juhend aadressil ( http://www.mbio.ncsu.edu/BioEdit/biodoc.pdf ).
    b. Laadida D.melanogaster püruvaatkinaasi mRNA järjestus programmi.
    c. Näppida programmi ja teha selgeks lihtsamad funktsioonid – selekteerimine, tühikute lisamine, redigeerimine jne.
    d. Leida nukleotiidide sisaldused, ORF-d, komplementaarsed järjestused, restriktsioonikaardid. Transleerida erinevates raamides, leida aminohapete sisaldused, luua erinevaid hüdrofoobsus ja –fiilsus profiile.
    Nukleotiidide sisaldused:
    DNA molecule: gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    Length = 2693 base pairs
    Molecular Weight = 818782,00 Daltons, single stranded
    Molecular Weight = 1636219,00 Daltons, double stranded
    G+C content = 48,05%
    A+T content = 51,95%
    Nucleotide Number Mol%
    A 880 32,68
    C 551 20,46
    G 743 27,59
    T 519 19,27
    ORF:
    >gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    ATGTTCCACTTGCCTGTTAAACCTAAGATATCGCTGCTATGCCAGCAAATGCAAGAAAGAGAAGAAACCACCGAAGTTCCGACTGGACCGCGGACCATTCGCCTCCCAGTTGGATTACCAGTCGCGACTGCAGTACCAAGCACCTGCCCTGAGTATCCCCCTCAGCAGCATCATATGCACCATTGGACCCTCATCCAACAGCCCAGAAAAGCTATTGGAGCTCATCCGGGCTGGGATGCGGGTCGTGCGTATGAACTTCTCTCACGGCTCTCACGAGTATCACTGCCAGACAATACAGGCGGCTCGTAA
    Komplementaarne järjestus:
    TACAAGGTGAACGGACAATTTGGATTCTATAGCGACGATACGGTCGTTTACGTTCTTTCTCTTCTTTGGTGGCTTCAAGGCTGACCTGGCGCCTGGTAAGCGGAGGGTCAACCTAATGGTCAGCGCTGACGTCATGGTTCGTGGACGGGACTCATAGGGGGAGTCGTCGTAGTATACGTGGTAACCTGGGAGTAGGTTGTCGGGTCTTTTCGATAACCTCGAGTAGGCCCGACCCTACGCCCAGCACGCATACTTGAAGAGAGTGCCGAGAGTGCTCATAGTGACGGTCTGTTATGTCCGCCGAGCATT
    Restriktsioonikaardid:
    BioEdit version 7.0.5.3 (10/28/05) Restriction Mapping Utility
    (c)1998, Tom Hall
    gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA Restriction Map
    27.02.2007 8:39:03
    2693 base pairs
    Translations: none
    Restriction Enzyme Map:
    1 TAAAATAAGCAAAAACTGTCCATTGTGATGTAAAATAAAAACAAAGCCACATAGTTTTAAATAAGTTTTAAGTAGGTATG 80
    1 ATTTTATTCGTTTTTGACAGGTAACACTACATTTTATTTTTGTTTCGGTGTATCAAAATTTATTCAAAATTCATCCATAC 80
    MslI DraI
    81 CGTTCTTAGGAATCAGGTCCCAGGTGTCACGGGTATACAAGGTGAACGGACAATTTGGATTCTATAGCGACGATACGGTC 160
    81 GCAAGAATCCTTAGTCCAGGGTCCACAGTGCCCATATGTTCCACTTGCCTGTTAAACCTAAGATATCGCTGCTATGCCAG 160
    BsmFI EcoO109I AccI Hpy8I HphI TspGWI DrdI
    PpuMI BstZ17I SfcI BsiEI
    NlaIV Hpy8I BaeI
    BsaJI
    161 GTTTACGTTCTTTCTCTTCTTTGGTGGCTTCAAGGCTGACCTGGCGCCTGGTAAGCGGAGGGTCAACCTAATGGTCAGCG 240
    161 CAAATGCAAGAAAGAGAAGAAACCACCGAAGTTCCGACTGGACCGCGGACCATTCGCCTCCCAGTTGGATTACCAGTCGC 240
    Hpy8I BaeI MwoI BbeI HpyF10VI HincII AfeI
    MboII EarI HpyF10VI MwoI Hpy8I
    BanI MnlI
    KasI
    BsaHI
    NarI
    NlaIV
    SfoI
    HaeII
    241 CTGACGTCATGGTTCGTGGACGGGACTCATAGGGGGAGTCGTCGTAGTATACGTGGTAACCTGGGAGTAGGTTGTCGGGT 320
    241 GACTGCAGTACCAAGCACCTGCCCTGAGTATCCCCCTCAGCAGCATCATATGCACCATTGGACCCTCATCCAACAGCCCA 320
    HaeII Hpy8I BsaXI BsmFI PleI BsaAI BsaJI
    BsaHI MlyI MlyI BstEII
    ZraI PleI AccI BsaXI
    AatII BstZ17I
    Hpy8I
    321 CTTTTCGATAACCTCGAGTAGGCCCGACCCTACGCCCAGCACGCATACTTGAAGAGAGTGCCGAGAGTGCTCATAGTGAC 400
    321 GAAAAGCTATTGGAGCTCATCCGGGCTGGGATGCGGGTCGTGCGTATGAACTTCTCTCACGGCTCTCACGAGTATCACTG 400
    AvaI MnlI BseYI EarI MboII BsiHKAI
    SmlI MwoI MwoI
    XhoI HpyF10VI HpyF10VI
    EcoNI Cac8I Bsp1286I
    BslI
    401 GGTCTGTTATGTCCGCCGAGCATTCCGGTAGCGGTACATGCACCTCGTTTGCCCAGACGGGTCTTGTAACCGTTAACGAG 480
    401 CCAGACAATACAGGCGGCTCGTAAGGCCATCGCCATGTACGTGGAGCAAACGGGTCTGCCCAGAACATTGGCAATTGCTC 480
    EciI BsmI MwoI MnlI BsaI
    BsaWI HpyF10VI BsmAI
    NspI HincII
    HpaI
    Hpy8I
    481 ACCTGTGGTTCCCAGGTCTCTAGGCCTGCCCGTTTGACCGGCCACCGTTACTATCCCGACTCTATCTCGAGTTCTGGCCT 560
    481 TGGACACCAAGGGTCCAGAGATCCGGACGGGCAAACTGGCCGGTGGCAATGATAGGGCTGAGATAGAGCTCAAGACCGGA 560
    NlaIV BsaI BsrFI Hin4I Hpy188III
    BsaJI BsmAI EaeI MlyI AvaI
    StuI PleI SmlI
    Cac8I Hpy188III XhoI
    561 CTGTTTCACTGTAACTCGTGGTTCTTCCTCTACCGTCTGTTCTCATTGTTCCTATTATAGATACACCTAATAGTCTCCGA 640
    561 GACAAAGTGACATTGAGCACCAAGAAGGAGATGGCAGACAAGAGTAACAAGGATAATATCTATGTGGATTATCAGAGGCT 640
    Hin4I MnlI BssSI MnlI BsmAI
    TspRI
    MboII
    641 TGGAGTTGACCAGTTTGGTCCTTTAGCCCATAAACATCTGCTGCCTAACTAACGTAACTAGCACTTCCTTAGTTTCCCTC 720
    641 ACCTCAACTGGTCAAACCAGGAAATCGGGTATTTGTAGACGACGGATTGATTGCATTGATCGTGAAGGAATCAAAGGGAG 720
    HincII BbvI
    Hpy8I
    BsrI
    721 TACTTCACTAGACAGTCCACCTCTTGCCACCGTTCGACCCGAGGGTGTTCCCTTAGTTAAACGGTCCTCAAGGCCATCTA 800
    721 ATGAAGTGATCTGTCAGGTGGAGAACGGTGGCAAGCTGGGCTCCCACAAGGGAATCAATTTGCCAGGAGTTCCGGTAGAT 800
    MnlI Hpy8I MnlI AvaI BpuEI SmlI MnlI
    MnlI XbaI
    BsaJI Hpy188III
    801 GAAGGGTCGCATTGCCTCTTCCTATTCGTCCTAGAATTTAAACCCCGGCTCGTTTTCCACCTGTACTAGAAGCGTAGCAA 880
    801 CTTCCCAGCGTAACGGAGAAGGATAAGCAGGATCTTAAATTTGGGGCCGAGCAAAAGGTGGACATGATCTTCGCATCGTT 880
    BsrDI EarI ApoI BsaJI TatI
    MboII MnlI DraI
    881 GTATGCTCTGCGTTTACGAGACTTCCTTTAGGCGGTTCATGAACCTGGCCGACCTCGCACATATTTCTAGTAGAGATTCT 960
    881 CATACGAGACGCAAATGCTCTGAAGGAAATCCGCCAAGTACTTGGACCGGCTGGAGCGTGTATAAAGATCATCTCTAAGA 960
    BsmAI TspDTI BspHI EaeI TspDTI MnlI
    Hpy8I BslI Hpy188III MwoI
    HpyF10VI
    961 AACTTTTGGTGGTTCCAGACCATTTGTATCTGCTGTAATAGGCACTTAGTCTACCGTATTACCACCGGGCTCCACTATAC 1040
    961 TTGAAAACCACCAAGGTCTGGTAAACATAGACGACATTATCCGTGAATCAGATGGCATAATGGTGGCCCGAGGTGATATG 1040
    NlaIV AccI NlaIV
    Hpy188III Hpy8I BanII
    Bsp1286I
    1041 CCGTAACTCTATGGGTGCCTCCTACAAGGGGATCGGGTCTTTAGCTAACACCGATTCACGTTGTTCCACCCGTTCGGACA 1120
    1041 GGCATTGAGATACCCACGGAGGATGTTCCCCTAGCCCAGAAATCGATTGTGGCTAAGTGCAACAAGGTGGGCAAGCCTGT 1120
    TaqII BanI BslI AlwI
    NlaIV MnlI
    1121 CTAGACACGGTGGGTCTACTACCTCAGCTACTGCTTGTTCGGCGCTGGGTGAGCACGGCTTCGGAGCCTACACCGGTTGC 1200
    1121 GATCTGTGCCACCCAGATGATGGAGTCGATGACGAACAAGCCGCGACCCACTCGTGCCGAAGCCTCGGATGTGGCCAACG 1200
    AccI BbvCI AlwNI BseMII HaeII BsiHKAI NlaIV AgeI
    Hpy8I Bpu10I MnlI MwoI MwoI HphI BceAI
    BspCNI BseYI HpyF10VI BsaWI
    TaqII Bsp1286I BsrFI
    HpyF10VI
    1201 GATAAAAGCTACCGACGCTACGGCATTACGACAGGCCACTTTGCCGGTTCCCGTTCATAGGCCACCTCACGCAGGTCACG 1280
    1201 CTATTTTCGATGGCTGCGATGCCGTAATGCTGTCCGGTGAAACGGCCAAGGGCAAGTATCCGGTGGAGTGCGTCCAGTGC 1280
    MwoI MwoI HgaI BceAI BsrFI BslI EcoNI BsaAI
    HpyF10VI TspDTI BspMI BslI
    HpyF10VI NlaIV MnlI
    1281 TACCGGTCCTAGACACGGTTTCATCTTCGTCAAGATACCATGCTTTCGGACGTCTTGTCGGAATTTTCCCTTTAGGCCTG 1360
    1281 ATGGCCAGGATCTGTGCCAAAGTAGAAGCAGTTCTATGGTACGAAAGCCTGCAGAACAGCCTTAAAAGGGAAATCCGGAC 1360
    AgeI TspDTI Hpy188III BsaHI ApoI Hin4I
    BsaWI BslI MmeI ZraI Hin4I
    BsrFI MboII Hin4I AatII StuI
    Hin4I AhdI
    1361 GTCACGACGGCTGGTGTAAAGCCGTCAATGGTGCCGCTAGCGCCTCCGCCGGTGCCACCCGGTTCGGGCCCGTTAACACC 1440
    1361 CAGTGCTGCCGACCACATTTCGGCAGTTACCACGGCGATCGCGGAGGCGGCCACGGTGGGCCAAGCCCGGGCAATTGTGG 1440
    Hpy188III BceAI BanI NheI HaeII BanI BslI ApaI
    NlaIV MwoI SgrAI PspOMI HincII
    EciI BsrFI NlaIV
    Cac8I MnlI BanII
    HpyF10VI NlaIV Bme1580I
    BmtI Bsp1286I
    HpaI
    Hpy8I
    1441 ACCGGTCAGGCACGAGGTACCACCGGGTTTACCAGTCAGTGTACTCCGGCGGTACAGGTTAACAGTACGAGTGCCCATTG 1520
    1441 TGGCCAGTCCGTGCTCCATGGTGGCCCAAATGGTCAGTCACATGAGGCCGCCATGTCCAATTGTCATGCTCACGGGTAAC 1520
    AgeI MnlI Acc65I Hpy8I TatI HincII Bme1580I
    BsaWI BssSI NlaIV BsrI Hpy8I HpaI Bsp1286I
    BsrFI BanI TspRI Hpy8I BsrDI
    KpnI EciI
    1521 CTTAGGCTCCGCCGGGTCAGAGACGAGAAAGCGCCGTAGATGGGTGAGGAGCAACTCCTTTACCAATAACCGTCGAAATT 1600
    1521 GAATCCGAGGCGGCCCAGTCTCTGCTCTTTCGCGGCATCTACCCACTCCTCGTTGAGGAAATGGTTATTGGCAGCTTTAA 1600
    Bpu10I BsmAI TaqII MnlI HphI BseRI BbvI
    NlaIV BsmBI HaeII
    BceAI BslI
    1601 GAAAGCTGCGTAGTACGTCAGCCCGGACTTCGACTACCCGTTCTACCTGTAGAACCTCGGCCCAGTCTTTCCTAGCCACC 1680
    1601 CTTTCGACGCATCATGCAGTCGGGCCTGAAGCTGATGGGCAAGATGGACATCTTGGAGCCGGGTCAGAAAGGATCGGTGG 1680
    SfcI BsaJI BsrI
    BmrI MnlI
    1681 ACGACCAATTACGGTACAGCCGTCTTTTCTAGTGGAAGGCCGACAAGTGATAGGCAGTCGTCTGGTTCCTTCTTGCGCTA 1760
    1681 TGCTGGTTAATGCCATGTCGGCAGAAAAGATCACCTTCCGGCTGTTCACTATCCGTCAGCAGACCAAGGAAGAACGCGAT 1760
    BceAI BaeI NlaIV
    BaeI Hin4I Hin4I
    Hin4I Hin4I
    1761 GTCCTACTCGCTACGGCTTTTAACCGGGATCTCGTCTCGACGTTCCTCTCCCGACTTTTCCTCCTTACGTCTTTCGAGGT 1840
    1761 CAGGATGAGCGATGCCGAAAATTGGCCCTAGAGCAGAGCTGCAAGGAGAGGGCTGAAAAGGAGGAATGCAGAAAGCTCCA 1840
    Hin4I BstYI AlwI Hin4I Hin4I MnlI Hin4I
    BceAI Hpy188III Hpy188III MnlI
    BsmBI MnlI BsaXI
    BsmAI
    BsaXI
    1841 CGTTCGCCTTCTTACAGTCTTCGTTTTTGACCGGTTTTTTACATTTGTCAAGCTTCTCTTCGTTTTCCAGACGGGGTTTT 1920
    1841 GCAAGCGGAAGAATGTCAGAAGCAAAAACTGGCCAAAAAATGTAAACAGTTCGAAGAGAAGCAAAAGGTCTGCCCCAAAA 1920
    MboII AgeI FalI MboII EarI FalI
    BbsI BsaWI HindIII Hpy188III
    BsrFI
    1921 TCTTACTATGAGGCTTCTTACTAACAGGCTTCTTTCTAACAGGCTTCTTTCTTACAGGGTTCGTTCTACTGCTTTAGAGT 2000
    1921 AGAATGATACTCCGAAGAATGATTGTCCGAAGAAAGATTGTCCGAAGAAAGAATGTCCCAAGCAAGATGACGAAATCTCA 2000
    MnlI TspGWI
    2001 TTCACGTCCGTTTACGTTCTTCGCCTTCGACTTCTTTCCTTCACGTTTCTTCTCAAACTTGTCTACACGTTTAATCGACT 2080
    2001 AAGTGCAGGCAAATGCAAGAAGCGGAAGCTGAAGAAAGGAAGTGCAAAGAAGAGTTTGAACAGATGTGCAAATTAGCTGA 2080
    BmgBI Hpy8I MboII AccI
    MboII Hpy8I
    AflIII
    2081 TCTTTTTTCCTTTCTTCGACTTTTTACGTCCTTTCGCCTGCTTCTTGCTTCCTTTCTCCTTCGACTTTTCACGTCCTTTA 2160
    2081 AGAAAAAAGGAAAGAAGCTGAAAAATGCAGGAAAGCGGACGAAGAACGAAGGAAAGAGGAAGCTGAAAAGTGCAGGAAAT 2160
    MboII Cac8I BmgBI
    MboII
    2161 ACCTTCTTCTATCCTTTACGTTTAACCGACTTCTCTTCTTTTCCTTACTTCTTCTTGAATTCTATTATCTTCGTCTTCAC 2240
    2161 TGGAAGAAGATAGGAAATGCAAATTGGCTGAAGAGAAGAAAAGGAATGAAGAAGAACTTAAGATAATAGAAGCAGAAGTG 2240
    MboII EarI Hpy188III MboII AgeI
    MboII ApoI BbsI BsaWI
    EcoRI HphI BsrFI
    MboII
    2241 CGGTTTGACCTTCGACGACTCTTCCGGTTCGCAAACTTCCTCCTTCTCTTTTTCTTCCTTCTTGATTACTTCACGTTCGT 2320
    2241 GCCAAACTGGAAGCTGCTGAGAAGGCCAAGCGTTTGAAGGAGGAAGAGAAAAAGAAGGAAGAACTAATGAAGTGCAAGCA 2320
    MlyI BsaWI MboII Hpy188III
    PleI EarI MnlI
    MboII
    2321 TGCTTTACTTCGCTTTTTTTTCTCCCTTCTCCGACTTTCCACGTTCTCTTTCCTTTCTCTTTCCCTCGATCGACTTTACC 2400
    2321 ACGAAATGAAGCGAAAAAAAAGAGGGAAGAGGCTGAAAGGTGCAAGAGAAAGGAAAGAGAAAGGGAGCTAGCTGAAATGG 2400
    MmeI BsiEI
    PvuI
    MnlI
    2401 TTTTGTTTACCTTTGTCCAACGCCTCTTTTCTTTCTCTTTCTTCCGTCGTCTCTACACGTCCTTTTATCTCCTACGTTTT 2480
    2401 AAAACAAATGGAAACAGGTTGCGGAGAAAAGAAAGAGAAAGAAGGCAGCAGAGATGTGCAGGAAAATAGAGGATGCAAAA 2480
    Hpy8I MboII MmeI BsmBI BceAI
    MnlI BsmAI
    TspGWI AflIII
    BmgBI
    2481 CTTTTTCGGCGCCGTCTTAGCCGACTATTCTAAGACTTTCGACACACGCTTTTTGATTTCGTTAGAGAGAGCCTAGGCCT 2560
    2481 GAAAAAGCCGCGGCAGAATCGGCTGATAAGATTCTGAAAGCTGTGTGCGAAAAACTAAAGCAATCTCTCTCGGATCCGGA 2560
    BanI MwoI AvrII
    KasI HpyF10VI BsaJI
    BsaHI StyI
    NarI StuI
    NlaIV
    SfoI
    BbeI
    HaeII
    2561 GTTTAGTTTTTTCCCGTTCTTTATTTCACTTTCGATTACCGTCTAAATATACCTTGTAAAATTGAAAATTATGCCACAAT 2640
    2561 CAAATCAAAAAAGGGCAAGAAATAAAGTGAAAGCTAATGGCAGATTTATATGGAACATTTTAACTTTTAATACGGTGTTA 2640
    2641 GTGTGGCTTAAGGTTTAATAATATATTTTGATAACCTTAAAAAATTTAAAAGA 2693
    2641 CACACCGAATTCCAAATTATTATATAAAACTATTGGAATTTTTTAAATTTTCT 2693
    DraIII ApoI
    AflII DraI
    SmlI
    Restriction table:
    Enzyme Recognition frequency Positions
    __________________________________________________________________________
    AatII G_ACGT'C 2 248, 1334
    AccI GT'mk_AC 5 115, 289, 1011, 1136, 2063
    Acc65I G'GTAC_C 1 1457
    AfeI AGC'GCT 1 240
    AflII C'TTAA_G 1 2648
    AflIII A'CryG_T 2 2066, 2456
    AgeI A'CCGG_T 5 1193 , 1283 , 1442 , 1871, 2240
    AhdI GACnn_n'nnGTC 1 1335
    AlwI GGATCnnnn'n_ 2 1079, 1796
    AlwNI CAG_nnn'CTG 1 1151
    ApaI G_GGCC'C 1 1431
    ApoI r'AATT_y 4 835, 1342, 2218, 2683
    AvaI C'yCGr_G 3 334, 547, 759
    AvrII C'CTAG_G 1 2553
    BaeI ACnnnnGTAyCnnnnnnn_nnnnn' 2 145, 1686
    BaeI GrTACnnnnGTnnnnnnnnnn_nnnnn' 2 178, 1719
    BanI G'GyrC_C 6 204, 1055, 1391, 1412 , 1457, 2489
    BanII G_rGCy'C 2 1032, 1431
    BbeI G_GCGC'C 2 208, 2493
    BbsI GAAGACnn'nnnn_ 2 1851 , 2227
    BbvI GCAGCnnnnnnnn'nnnn_ 2 668, 1593
    BbvCI CC'TCA_GC 1 1144
    BceAI ACGGCnnnnnnnnnnnn'nn_ 7 1192 , 1237 , 1384, 1539 , 1685 , 1790
    2477
    Bme1580I G_kGCm'C 2 1431, 1516
    BmgBI CAC'GTC 3 2006, 2153, 2459
    BmrI ACTGGGnnnn_n' 1 1657
    BmtI G_CTAG'C 1 1401
    Bpu10I CC'TnA_GC 2 1144, 1522
    BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 773
    BsaI GGTCTCn'nnnn_ 2 473, 502
    BsaAI yAC'GTr 2 293, 1280
    BsaHI Gr'CG_yC 4 205, 245, 1331 , 2490
    BsaJI C'CnnG_G 7 100, 301, 492, 760, 844, 1656
    2553
    BsaWI w'CCGG_w 7 425, 1193, 1283, 1442, 1871, 2240
    2264
    BsaXI ACnnnnnCTCCnnnnnnn_nnn' 2 268, 1835
    BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 2 298, 1805
    BseMII CTCAGnnnnnnnn_nn' 1 1158
    BseRI GAGGAGnnnnnnnn_nn' 1 1582
    BseYI C'CCAG_C 2 356, 1165
    BsiEI CG_ry'CG 2 160, 2391
    BsiHKAI G_wGCw'C 2 392, 1176
    BslI CCnn_nnn'nnGG 8 339, 912, 1068, 1258, 1272, 1295
    1425, 1561
    BsmI GAATG_Cn' 1 421
    BsmAI GTCTCn'nnnn_ 7 473, 502, 639, 893, 1535, 1800
    2455
    BsmBI CGTCTCn'nnnn_ 3 1535, 1800, 2455
    BsmFI GGGACnnnnnnnnnn'nnnn_ 2 83, 277
    Bsp1286I G_dGCh'C 5 392, 1032, 1176, 1431, 1516
    BspCNI CTCAGnnnnnnn_nn' 1 1157
    BspHI T'CATG_A 1 918
    BspMI ACCTGCnnnn'nnnn_ 1 1263
    BsrI ACTG_Gn' 3 651, 1473 , 1663
    BsrDI GCAATG_nn' 2 810, 1516
    BsrFI r'CCGG_y 8 518, 1193, 1244, 1283, 1409, 1442
    1871, 2240
    BssSI C'ACGA_G 2 576, 1452
    BstEII G'GTnAC_C 1 296
    BstYI r'GATC_y 1 1788
    BstZ17I GTA'TAC 2 116, 290
    Cac8I GCn'nGC 4 362, 507, 1399, 2119
    DraI TTT'AAA 3 59, 840, 2688
    DraIII CAC_nnn'GTG 1 2641
    DrdI GACnn_nn'nnGTC 1 156
    EaeI y'GGCC_r 2 520, 927
    EarI CTCTTCn'nnn_ 6 181, 367, 823, 1903, 2200 , 2266
    EciI GGCGGAnnnnnnnnn_nn' 3 403, 1396 , 1519
    EcoNI CCTnn'n_nnAGG 2 337, 1270
    EcoO109I rG'GnC_Cy 1 97
    EcoRI G'AATT_C 1 2218
    FalI AAGnnnnnCTTnnnnnnnn_nnnnn' 2 1882, 1914
    HaeII r_GCGC'y 6 208, 242, 1166, 1404, 1555 , 2493
    HgaI GACGCnnnnn'nnnnn_ 1 1224
    Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 7 562, 1321, 1353, 1721, 1753, 1812
    1837
    Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 7 530, 1321, 1353, 1721, 1753, 1780
    1805
    HincII GTy'rAC 5 225, 475, 648, 1435, 1501
    HindIII A'AGCT_T 1 1891
    HpaI GTT'AAC 3 475, 1435, 1501
    HphI GGTGAnnnnnnn_n' 4 134, 1181, 1576 , 2230
    Hpy8I GTn'nAC 19 116, 125, 164, 225, 259, 290
    475, 648, 738, 895, 1012, 1137
    1435, 1470, 1482 , 1501, 2013
    2064, 2408
    Hpy188III TC'nn_GA 12 536, 547, 799, 919, 976, 1312
    1364, 1797, 1811, 1908, 2215
    2302
    HpyF10VI GCn_nnnnn'nGC 12 203, 214, 362, 388, 439, 936
    1161, 1172, 1207, 1216, 1401
    2499
    KasI G'GCGC_C 2 204, 2489
    KpnI G_GTAC'C 1 1461
    MboII GAAGAnnnnnnn_n' 18 168, 384, 576, 810, 1297 , 1851
    1890, 2011, 2041, 2086, 2158
    2187, 2203, 2221, 2227, 2253
    2286, 2433
    MlyI GAGTCnnnnn' 4 259, 286, 533, 2252
    MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 1319, 2376, 2442
    MnlI CCTCnnnnnn_n' 25 212, 343, 454, 569, 598, 728
    751, 755, 797, 826, 944, 1069
    1153, 1276, 1414, 1448, 1560
    1666 , 1816, 1831 , 1830, 1924
    2290, 2395, 2434
    MslI CAynn'nnrTG 1 26
    MwoI GCnn_nnn'nnGC 12 202, 213, 361, 387, 438, 935
    1160, 1171, 1206 , 1215 , 1400
    2498
    NarI GG'CG_CC 2 205, 2490
    NheI G'CTAG_C 1 1397
    NlaIV GGn'nCC 15 99, 206, 490, 974, 1031, 1057
    1186 , 1249, 1393, 1414, 1429
    1459, 1528, 1747, 2491
    NspI r_CATG'y 1 441
    PleI GAGTCnnnn'n_ 4 259, 285, 533, 2252
    PpuMI rG'GwC_Cy 1 97
    PspOMI G'GGCC_C 1 1427
    PvuI CG_AT'CG 1 2391
    SfcI C'TryA_G 2 143, 1648
    SfoI GGC'GCC 2 206, 2491
    SgrAI Cr'CCGG_yG 1 1409
    SmlI C'TyrA_G 4 334, 547, 788, 2648
    StuI AGG'CCT 3 505, 1357, 2558
    StyI C'CwwG_G 1 2553
    TaqII CACCCAnnnnnnnnn_nn' 3 1043, 1157, 1552
    TatI w'GTAC_w 2 863, 1481
    TspDTI ATGAAnnnnnnnnn_nn' 4 907, 935, 1245, 1291
    TspGWI ACGGAnnnnnnnnn_nn' 3 142, 1998, 2434
    TspRI _nnCAsTGnn' 2 574, 1484
    XbaI T'CTAG_A 1 798
    XhoI C'TCGA_G 2 334, 547
    ZraI GAC'GTC 2 246, 1332
    Enzymes that cut five or fewer times
    Enzyme Recognition frequency Positions
    __________________________________________________________________________
    AatII G_ACGT'C 2 248, 1334
    AccI GT'mk_AC 5 115, 289, 1011, 1136, 2063
    Acc65I G'GTAC_C 1 1457
    AfeI AGC'GCT 1 240
    AflII C'TTAA_G 1 2648
    AflIII A'CryG_T 2 2066, 2456
    AgeI A'CCGG_T 5 1193, 1283, 1442, 1871, 2240
    AhdI GACnn_n'nnGTC 1 1335
    AlwI GGATCnnnn'n_ 2 1079, 1796
    AlwNI CAG_nnn'CTG 1 1151
    ApaI G_GGCC'C 1 1431
    ApoI r'AATT_y 4 835, 1342, 2218, 2683
    AvaI C'yCGr_G 3 334, 547, 759
    AvrII C'CTAG_G 1 2553
    BaeI ACnnnnGTAyCnnnnnnn_nnnnn' 2 145, 1686
    BaeI GrTACnnnnGTnnnnnnnnnn_nnnnn' 2 178, 1719
    BanII G_rGCy'C 2 1032, 1431
    BbeI G_GCGC'C 2 208, 2493
    BbsI GAAGACnn'nnnn_ 2 1851, 2227
    BbvI GCAGCnnnnnnnn'nnnn_ 2 668, 1593
    BbvCI CC'TCA_GC 1 1144
    Bme1580I G_kGCm'C 2 1431, 1516
    BmgBI CAC'GTC 3 2006, 2153, 2459
    BmrI ACTGGGnnnn_n' 1 1657
    BmtI G_CTAG'C 1 1401
    Bpu10I CC'TnA_GC 2 1144, 1522
    BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 773
    BsaI GGTCTCn'nnnn_ 2 473, 502
    BsaAI yAC'GTr 2 293, 1280
    BsaHI Gr'CG_yC 4 205, 245, 1331, 2490
    BsaXI ACnnnnnCTCCnnnnnnn_nnn' 2 268, 1835
    BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 2 298, 1805
    BseMII CTCAGnnnnnnnn_nn' 1 1158
    BseRI GAGGAGnnnnnnnn_nn' 1 1582
    BseYI C'CCAG_C 2 356, 1165
    BsiEI CG_ry'CG 2 160, 2391
    BsiHKAI G_wGCw'C 2 392, 1176
    BsmI GAATG_Cn' 1 421
    BsmBI CGTCTCn'nnnn_ 3 1535, 1800, 2455
    BsmFI GGGACnnnnnnnnnn'nnnn_ 2 83, 277
    Bsp1286I G_dGCh'C 5 392, 1032, 1176, 1431, 1516
    BspCNI CTCAGnnnnnnn_nn' 1 1157
    BspHI T'CATG_A 1 918
    BspMI ACCTGCnnnn'nnnn_ 1 1263
    BsrI ACTG_Gn' 3 651, 1473, 1663
    BsrDI GCAATG_nn' 2 810, 1516
    BssSI C'ACGA_G 2 576, 1452
    BstEII G'GTnAC_C 1 296
    BstYI r'GATC_y 1 1788
    BstZ17I GTA'TAC 2 116, 290
    Cac8I GCn'nGC 4 362, 507, 1399, 2119
    DraI TTT'AAA 3 59, 840, 2688
    DraIII CAC_nnn'GTG 1 2641
    DrdI GACnn_nn'nnGTC 1 156
    EaeI y'GGCC_r 2 520, 927
    EciI GGCGGAnnnnnnnnn_nn' 3 403, 1396, 1519
    EcoNI CCTnn'n_nnAGG 2 337, 1270
    EcoO109I rG'GnC_Cy 1 97
    EcoRI G'AATT_C 1 2218
    FalI AAGnnnnnCTTnnnnnnnn_nnnnn' 2 1882, 1914
    HgaI GACGCnnnnn'nnnnn_ 1 1224
    HincII GTy'rAC 5 225, 475, 648, 1435, 1501
    HindIII A'AGCT_T 1 1891
    HpaI GTT'AAC 3 475, 1435, 1501
    HphI GGTGAnnnnnnn_n' 4 134, 1181, 1576, 2230
    KasI G'GCGC_C 2 204, 2489
    KpnI G_GTAC'C 1 1461
    MlyI GAGTCnnnnn' 4 259, 286, 533, 2252
    MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 1319, 2376, 2442
    MslI CAynn'nnrTG 1 26
    NarI GG'CG_CC 2 205, 2490
    NheI G'CTAG_C 1 1397
    NspI r_CATG'y 1 441
    PleI GAGTCnnnn'n_ 4 259, 285, 533, 2252
    PpuMI rG'GwC_Cy 1 97
    PspOMI G'GGCC_C 1 1427
    PvuI CG_AT'CG 1 2391
    SfcI C'TryA_G 2 143, 1648
    SfoI GGC'GCC 2 206, 2491
    SgrAI Cr'CCGG_yG 1 1409
    SmlI C'TyrA_G 4 334, 547, 788, 2648
    StuI AGG'CCT 3 505, 1357, 2558
    StyI C'CwwG_G 1 2553
    TaqII CACCCAnnnnnnnnn_nn' 3 1043, 1157, 1552
    TatI w'GTAC_w 2 863, 1481
    TspDTI ATGAAnnnnnnnnn_nn' 4 907, 935, 1245, 1291
    TspGWI ACGGAnnnnnnnnn_nn' 3 142, 1998, 2434
    TspRI _nnCAsTGnn' 2 574, 1484
    XbaI T'CTAG_A 1 798
    XhoI C'TCGA_G 2 334, 547
    ZraI GAC'GTC 2 246, 1332
    Position Enzyme(s)
    __________________________________________________________________________
    26 MslI CAynn'nnrTG
    59 DraI TTT'AAA
    83 BsmFI GGGACnnnnnnnnnn'nnnn_
    97 EcoO109I rG'GnC_Cy
    97 PpuMI rG'GwC_Cy
    99 NlaIV GGn'nCC
    100 BsaJI C'CnnG_G
    115 AccI GT'mk_AC
    116 BstZ17I GTA'TAC
    116 Hpy8I GTn'nAC
    125 Hpy8I GTn'nAC
    134 HphI GGTGAnnnnnnn_n'
    142 TspGWI ACGGAnnnnnnnnn_nn'
    143 SfcI C'TryA_G
    145 BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
    156 DrdI GACnn_nn'nnGTC
    160 BsiEI CG_ry'CG
    164 Hpy8I GTn'nAC
    168 MboII GAAGAnnnnnnn_n'
    178 BaeI GrTACnnnnGTnnnnnnnnnn_nnnnn'
    181 EarI CTCTTCn'nnn_
    202 MwoI GCnn_nnn'nnGC
    203 HpyF10VI GCn_nnnnn'nGC
    204 BanI G'GyrC_C
    204 KasI G'GCGC_C
    205 BsaHI Gr'CG_yC
    205 NarI GG'CG_CC
    206 NlaIV GGn'nCC
    206 SfoI GGC'GCC
    208 BbeI G_GCGC'C
    208 HaeII r_GCGC'y
    212 MnlI CCTCnnnnnn_n'
    213 MwoI GCnn_nnn'nnGC
    214 HpyF10VI GCn_nnnnn'nGC
    225 HincII GTy'rAC
    225 Hpy8I GTn'nAC
    240 AfeI AGC'GCT
    242 HaeII r_GCGC'y
    245 BsaHI Gr'CG_yC
    246 ZraI GAC'GTC
    248 AatII G_ACGT'C
    259 Hpy8I GTn'nAC
    259 MlyI GAGTCnnnnn'
    259 PleI GAGTCnnnn'n_
    268 BsaXI ACnnnnnCTCCnnnnnnn_nnn'
    277 BsmFI GGGACnnnnnnnnnn'nnnn_
    285 PleI GAGTCnnnn'n_
    286 MlyI GAGTCnnnnn'
    289 AccI GT'mk_AC
    290 BstZ17I GTA'TAC
    290 Hpy8I GTn'nAC
    293 BsaAI yAC'GTr
    296 BstEII G'GTnAC_C
    298 BsaXI GGAGnnnnnGTnnnnnnnnn_nnn'
    301 BsaJI C'CnnG_G
    334 AvaI C'yCGr_G
    334 SmlI C'TyrA_G
    334 XhoI C'TCGA_G
    337 EcoNI CCTnn'n_nnAGG
    339 BslI CCnn_nnn'nnGG
    343 MnlI CCTCnnnnnn_n'
    356 BseYI C'CCAG_C
    361 MwoI GCnn_nnn'nnGC
    362 HpyF10VI GCn_nnnnn'nGC
    362 Cac8I GCn'nGC
    367 EarI CTCTTCn'nnn_
    384 MboII GAAGAnnnnnnn_n'
    387 MwoI GCnn_nnn'nnGC
    388 HpyF10VI GCn_nnnnn'nGC
    392 BsiHKAI G_wGCw'C
    392 Bsp1286I G_dGCh'C
    403 EciI GGCGGAnnnnnnnnn_nn'
    421 BsmI GAATG_Cn'
    425 BsaWI w'CCGG_w
    438 MwoI GCnn_nnn'nnGC
    439 HpyF10VI GCn_nnnnn'nGC
    441 NspI r_CATG'y
    454 MnlI CCTCnnnnnn_n'
    473 BsaI GGTCTCn'nnnn_
    473 BsmAI GTCTCn'nnnn_
    475 HincII GTy'rAC
    475 HpaI GTT'AAC
    475 Hpy8I GTn'nAC
    490 NlaIV GGn'nCC
    492 BsaJI C'CnnG_G
    502 BsaI GGTCTCn'nnnn_
    502 BsmAI GTCTCn'nnnn_
    505 StuI AGG'CCT
    507 Cac8I GCn'nGC
    518 BsrFI r'CCGG_y
    520 EaeI y'GGCC_r
    530 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    533 MlyI GAGTCnnnnn'
    533 PleI GAGTCnnnn'n_
    536 Hpy188III TC'nn_GA
    547 Hpy188III TC'nn_GA
    547 AvaI C'yCGr_G
    547 SmlI C'TyrA_G
    547 XhoI C'TCGA_G
    562 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    569 MnlI CCTCnnnnnn_n'
    574 TspRI _nnCAsTGnn'
    576 BssSI C'ACGA_G
    576 MboII GAAGAnnnnnnn_n'
    598 MnlI CCTCnnnnnn_n'
    639 BsmAI GTCTCn'nnnn_
    648 HincII GTy'rAC
    648 Hpy8I GTn'nAC
    651 BsrI ACTG_Gn'
    668 BbvI GCAGCnnnnnnnn'nnnn_
    728 MnlI CCTCnnnnnn_n'
    738 Hpy8I GTn'nAC
    751 MnlI CCTCnnnnnn_n'
    755 MnlI CCTCnnnnnn_n'
    759 AvaI C'yCGr_G
    760 BsaJI C'CnnG_G
    773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
    788 SmlI C'TyrA_G
    797 MnlI CCTCnnnnnn_n'
    798 XbaI T'CTAG_A
    799 Hpy188III TC'nn_GA
    810 BsrDI GCAATG_nn'
    810 MboII GAAGAnnnnnnn_n'
    823 EarI CTCTTCn'nnn_
    826 MnlI CCTCnnnnnn_n'
    835 ApoI r'AATT_y
    840 DraI TTT'AAA
    844 BsaJI C'CnnG_G
    863 TatI w'GTAC_w
    893 BsmAI GTCTCn'nnnn_
    895 Hpy8I GTn'nAC
    907 TspDTI ATGAAnnnnnnnnn_nn'
    912 BslI CCnn_nnn'nnGG
    918 BspHI T'CATG_A
    919 Hpy188III TC'nn_GA
    927 EaeI y'GGCC_r
    935 TspDTI ATGAAnnnnnnnnn_nn'
    935 MwoI GCnn_nnn'nnGC
    936 HpyF10VI GCn_nnnnn'nGC
    944 MnlI CCTCnnnnnn_n'
    974 NlaIV GGn'nCC
    976 Hpy188III TC'nn_GA
    1011 AccI GT'mk_AC
    1012 Hpy8I GTn'nAC
    1031 NlaIV GGn'nCC
    1032 BanII G_rGCy'C
    1032 Bsp1286I G_dGCh'C
    1043 TaqII CACCCAnnnnnnnnn_nn'
    1055 BanI G'GyrC_C
    1057 NlaIV GGn'nCC
    1068 BslI CCnn_nnn'nnGG
    1069 MnlI CCTCnnnnnn_n'
    1079 AlwI GGATCnnnn'n_
    1136 AccI GT'mk_AC
    1137 Hpy8I GTn'nAC
    1144 BbvCI CC'TCA_GC
    1144 Bpu10I CC'TnA_GC
    1151 AlwNI CAG_nnn'CTG
    1153 MnlI CCTCnnnnnn_n'
    1157 BspCNI CTCAGnnnnnnn_nn'
    1157 TaqII CACCCAnnnnnnnnn_nn'
    1158 BseMII CTCAGnnnnnnnn_nn'
    1160 MwoI GCnn_nnn'nnGC
    1161 HpyF10VI GCn_nnnnn'nGC
    1165 BseYI C'CCAG_C
    1166 HaeII r_GCGC'y
    1171 MwoI GCnn_nnn'nnGC
    1172 HpyF10VI GCn_nnnnn'nGC
    1176 BsiHKAI G_wGCw'C
    1176 Bsp1286I G_dGCh'C
    1181 HphI GGTGAnnnnnnn_n'
    1186 NlaIV GGn'nCC
    1192 BceAI ACGGCnnnnnnnnnnnn'nn_
    1193 AgeI A'CCGG_T
    1193 BsaWI w'CCGG_w
    1193 BsrFI r'CCGG_y
    1206 MwoI GCnn_nnn'nnGC
    1207 HpyF10VI GCn_nnnnn'nGC
    1215 MwoI GCnn_nnn'nnGC
    1216 HpyF10VI GCn_nnnnn'nGC
    1224 HgaI GACGCnnnnn'nnnnn_
    1237 BceAI ACGGCnnnnnnnnnnnn'nn_
    1244 BsrFI r'CCGG_y
    1245 TspDTI ATGAAnnnnnnnnn_nn'
    1249 NlaIV GGn'nCC
    1258 BslI CCnn_nnn'nnGG
    1263 BspMI ACCTGCnnnn'nnnn_
    1270 EcoNI CCTnn'n_nnAGG
    1272 BslI CCnn_nnn'nnGG
    1276 MnlI CCTCnnnnnn_n'
    1280 BsaAI yAC'GTr
    1283 AgeI A'CCGG_T
    1283 BsaWI w'CCGG_w
    1283 BsrFI r'CCGG_y
    1291 TspDTI ATGAAnnnnnnnnn_nn'
    1295 BslI CCnn_nnn'nnGG
    1297 MboII GAAGAnnnnnnn_n'
    1312 Hpy188III TC'nn_GA
    1319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
    1321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1321 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1331 BsaHI Gr'CG_yC
    1332 ZraI GAC'GTC
    1334 AatII G_ACGT'C
    1335 AhdI GACnn_n'nnGTC
    1342 ApoI r'AATT_y
    1353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1353 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1357 StuI AGG'CCT
    1364 Hpy188III TC'nn_GA
    1384 BceAI ACGGCnnnnnnnnnnnn'nn_
    1391 BanI G'GyrC_C
    1393 NlaIV GGn'nCC
    1396 EciI GGCGGAnnnnnnnnn_nn'
    1397 NheI G'CTAG_C
    1399 Cac8I GCn'nGC
    1400 MwoI GCnn_nnn'nnGC
    1401 HpyF10VI GCn_nnnnn'nGC
    1401 BmtI G_CTAG'C
    1404 HaeII r_GCGC'y
    1409 SgrAI Cr'CCGG_yG
    1409 BsrFI r'CCGG_y
    1412 BanI G'GyrC_C
    1414 MnlI CCTCnnnnnn_n'
    1414 NlaIV GGn'nCC
    1425 BslI CCnn_nnn'nnGG
    1427 PspOMI G'GGCC_C
    1429 NlaIV GGn'nCC
    1431 ApaI G_GGCC'C
    1431 BanII G_rGCy'C
    1431 Bme1580I G_kGCm'C
    1431 Bsp1286I G_dGCh'C
    1435 HincII GTy'rAC
    1435 HpaI GTT'AAC
    1435 Hpy8I GTn'nAC
    1442 AgeI A'CCGG_T
    1442 BsaWI w'CCGG_w
    1442 BsrFI r'CCGG_y
    1448 MnlI CCTCnnnnnn_n'
    1452 BssSI C'ACGA_G
    1457 Acc65I G'GTAC_C
    1457 BanI G'GyrC_C
    1459 NlaIV GGn'nCC
    1461 KpnI G_GTAC'C
    1470 Hpy8I GTn'nAC
    1473 BsrI ACTG_Gn'
    1481 TatI w'GTAC_w
    1482 Hpy8I GTn'nAC
    1484 TspRI _nnCAsTGnn'
    1501 HincII GTy'rAC
    1501 HpaI GTT'AAC
    1501 Hpy8I GTn'nAC
    1516 Bme1580I G_kGCm'C
    1516 Bsp1286I G_dGCh'C
    1516 BsrDI GCAATG_nn'
    1519 EciI GGCGGAnnnnnnnnn_nn'
    1522 Bpu10I CC'TnA_GC
    1528 NlaIV GGn'nCC
    1535 BsmAI GTCTCn'nnnn_
    1535 BsmBI CGTCTCn'nnnn_
    1539 BceAI ACGGCnnnnnnnnnnnn'nn_
    1552 TaqII CACCCAnnnnnnnnn_nn'
    1555 HaeII r_GCGC'y
    1560 MnlI CCTCnnnnnn_n'
    1561 BslI CCnn_nnn'nnGG
    1576 HphI GGTGAnnnnnnn_n'
    1582 BseRI GAGGAGnnnnnnnn_nn'
    1593 BbvI GCAGCnnnnnnnn'nnnn_
    1648 SfcI C'TryA_G
    1656 BsaJI C'CnnG_G
    1657 BmrI ACTGGGnnnn_n'
    1663 BsrI ACTG_Gn'
    1666 MnlI CCTCnnnnnn_n'
    1685 BceAI ACGGCnnnnnnnnnnnn'nn_
    1686 BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
    1719 BaeI GrTACnnnnGTnnnnnnnnnn_nnnnn'
    1721 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1721 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1747 NlaIV GGn'nCC
    1753 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1753 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1780 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1788 BstYI r'GATC_y
    1790 BceAI ACGGCnnnnnnnnnnnn'nn_
    1796 AlwI GGATCnnnn'n_
    1797 Hpy188III TC'nn_GA
    1800 BsmBI CGTCTCn'nnnn_
    1800 BsmAI GTCTCn'nnnn_
    1805 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
    1805 BsaXI GGAGnnnnnGTnnnnnnnnn_nnn'
    1811 Hpy188III TC'nn_GA
    1812 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1816 MnlI CCTCnnnnnn_n'
    1830 MnlI CCTCnnnnnn_n'
    1831 MnlI CCTCnnnnnn_n'
    1835 BsaXI ACnnnnnCTCCnnnnnnn_nnn'
    1837 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
    1851 MboII GAAGAnnnnnnn_n'
    1851 BbsI GAAGACnn'nnnn_
    1871 AgeI A'CCGG_T
    1871 BsaWI w'CCGG_w
    1871 BsrFI r'CCGG_y
    1882 FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
    1890 MboII GAAGAnnnnnnn_n'
    1891 HindIII A'AGCT_T
    1903 EarI CTCTTCn'nnn_
    1908 Hpy188III TC'nn_GA
    1914 FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
    1924 MnlI CCTCnnnnnn_n'
    1998 TspGWI ACGGAnnnnnnnnn_nn'
    2006 BmgBI CAC'GTC
    2011 MboII GAAGAnnnnnnn_n'
    2013 Hpy8I GTn'nAC
    2041 MboII GAAGAnnnnnnn_n'
    2063 AccI GT'mk_AC
    2064 Hpy8I GTn'nAC
    2066 AflIII A'CryG_T
    2086 MboII GAAGAnnnnnnn_n'
    2119 Cac8I GCn'nGC
    2153 BmgBI CAC'GTC
    2158 MboII GAAGAnnnnnnn_n'
    2187 MboII GAAGAnnnnnnn_n'
    2200 EarI CTCTTCn'nnn_
    2203 MboII GAAGAnnnnnnn_n'
    2215 Hpy188III TC'nn_GA
    2218 ApoI r'AATT_y
    2218 EcoRI G'AATT_C
    2221 MboII GAAGAnnnnnnn_n'
    2227 MboII GAAGAnnnnnnn_n'
    2227 BbsI GAAGACnn'nnnn_
    2230 HphI GGTGAnnnnnnn_n'
    2240 AgeI A'CCGG_T
    2240 BsaWI w'CCGG_w
    2240 BsrFI r'CCGG_y
    2252 MlyI GAGTCnnnnn'
    2252 PleI GAGTCnnnn'n_
    2253 MboII GAAGAnnnnnnn_n'
    2264 BsaWI w'CCGG_w
    2266 EarI CTCTTCn'nnn_
    2286 MboII GAAGAnnnnnnn_n'
    2290 MnlI CCTCnnnnnn_n'
    2302 Hpy188III TC'nn_GA
    2376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
    2391 BsiEI CG_ry'CG
    2391 PvuI CG_AT'CG
    2395 MnlI CCTCnnnnnn_n'
    2408 Hpy8I GTn'nAC
    2433 MboII GAAGAnnnnnnn_n'
    2434 MnlI CCTCnnnnnn_n'
    2434 TspGWI ACGGAnnnnnnnnn_nn'
    2442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
    2455 BsmBI CGTCTCn'nnnn_
    2455 BsmAI GTCTCn'nnnn_
    2456 AflIII A'CryG_T
    2459 BmgBI CAC'GTC
    2477 BceAI ACGGCnnnnnnnnnnnn'nn_
    2489 BanI G'GyrC_C
    2489 KasI G'GCGC_C
    2490 BsaHI Gr'CG_yC
    2490 NarI GG'CG_CC
    2491 NlaIV GGn'nCC
    2491 SfoI GGC'GCC
    2493 BbeI G_GCGC'C
    2493 HaeII r_GCGC'y
    2498 MwoI GCnn_nnn'nnGC
    2499 HpyF10VI GCn_nnnnn'nGC
    2553 AvrII C'CTAG_G
    2553 BsaJI C'CnnG_G
    2553 StyI C'CwwG_G
    2558 StuI AGG'CCT
    2641 DraIII CAC_nnn'GTG
    2648 AflII C'TTAA_G
    2648 SmlI C'TyrA_G
    2683 ApoI r'AATT_y
    2688 DraI TTT'AAA
    Enzymes that do not cut:
    _________________________________________________________
    AarI, AclI, AleI, AloI, AloI, ApaLI, AscI, AseI, AsiSI, BamHI, BcgI, BcgI, BciVI
    BclI, BfrBI, BglI, BglII, BlpI, BplI, BpmI, BsaBI, BsgI, BsiWI, BspEI, BsrBI
    BsrGI, BssHII, BstAPI, BstBI, BstF5I, BstXI, Bsu36I, BtgI, BtsI, ClaI, EagI
    Eco57I, EcoICRI, Eco57MI, EcoRV, FauI, FokI, FseI, FspI, FspAI, MfeI, MluI, MscI
    MspA1I, NaeI, NcoI, NdeI, NgoMIV, NotI, NruI, NsiI, PacI, PciI, PflMI, PmeI, PmlI
    PpiI, PpiI, PshAI, PsiI, PsrI, PsrI, PstI, PvuII, RsrII, SacI, SacII, SalI, SanDI
    SapI , SbfI, ScaI, SexAI, SfaNI, SfiI, SmaI, SnaBI, SpeI, SphI, SrfI, SspI, SwaI
    TaqII, Tth111I, XcmI, XmaI, XmnI
    1 Transleerimise raam:
    >gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    1 TAA AAT AAG CAA AAA CTG TCC ATT GTG ATG TAA AAT AAA AAC AAA 45
    1 End Asn Lys Gln Lys Leu Ser Ile Val Met End Asn Lys Asn Lys 15
    46 GCC ACA TAG TTT TAA ATA AGT TTT AAG TAG GTA TGC GTT CTT AGG 90
    16 Ala Thr End Phe End Ile Ser Phe Lys End Val Cys Val Leu Arg 30
    91 AAT CAG GTC CCA GGT GTC ACG GGT ATA CAA GGT GAA CGG ACA ATT 135
    31 Asn Gln Val Pro Gly Val Thr Gly Ile Gln Gly Glu Arg Thr Ile 45
    136 TGG ATT CTA TAG CGA CGA TAC GGT CGT TTA CGT TCT TTC TCT TCT 180
    46 Trp Ile Leu End Arg Arg Tyr Gly Arg Leu Arg Ser Phe Ser Ser 60
    181 TTG GTG GCT TCA AGG CTG ACC TGG CGC CTG GTA AGC GGA GGG TCA 225
    61 Leu Val Ala Ser Arg Leu Thr Trp Arg Leu Val Ser Gly Gly Ser 75
    226 ACC TAA TGG TCA GCG CTG ACG TCA TGG TTC GTG GAC GGG ACT CAT 270
    76 Thr End Trp Ser Ala Leu Thr Ser Trp Phe Val Asp Gly Thr His 90
    271 AGG GGG AGT CGT CGT AGT ATA CGT GGT AAC CTG GGA GTA GGT TGT 315
    91 Arg Gly Ser Arg Arg Ser Ile Arg Gly Asn Leu Gly Val Gly Cys 105
    316 CGG GTC TTT TCG ATA ACC TCG AGT AGG CCC GAC CCT ACG CCC AGC 360
    106 Arg Val Phe Ser Ile Thr Ser Ser Arg Pro Asp Pro Thr Pro Ser 120
    361 ACG CAT ACT TGA AGA GAG TGC CGA GAG TGC TCA TAG TGA CGG TCT 405
    121 Thr His Thr End Arg Glu Cys Arg Glu Cys Ser End End Arg Ser 135
    406 GTT ATG TCC GCC GAG CAT TCC GGT AGC GGT ACA TGC ACC TCG TTT 450
    136 Val Met Ser Ala Glu His Ser Gly Ser Gly Thr Cys Thr Ser Phe 150
    451 GCC CAG ACG GGT CTT GTA ACC GTT AAC GAG ACC TGT GGT TCC CAG 495
    151 Ala Gln Thr Gly Leu Val Thr Val Asn Glu Thr Cys Gly Ser Gln 165
    496 GTC TCT AGG CCT GCC CGT TTG ACC GGC CAC CGT TAC TAT CCC GAC 540
    166 Val Ser Arg Pro Ala Arg Leu Thr Gly His Arg Tyr Tyr Pro Asp 180
    541 TCT ATC TCG AGT TCT GGC CTC TGT TTC ACT GTA ACT CGT GGT TCT 585
    181 Ser Ile Ser Ser Ser Gly Leu Cys Phe Thr Val Thr Arg Gly Ser 195
    586 TCC TCT ACC GTC TGT TCT CAT TGT TCC TAT TAT AGA TAC ACC TAA 630
    196 Ser Ser Thr Val Cys Ser His Cys Ser Tyr Tyr Arg Tyr Thr End 210
    631 TAG TCT CCG ATG GAG TTG ACC AGT TTG GTC CTT TAG CCC ATA AAC 675
    211 End Ser Pro Met Glu Leu Thr Ser Leu Val Leu End Pro Ile Asn 225
    676 ATC TGC TGC CTA ACT AAC GTA ACT AGC ACT TCC TTA GTT TCC CTC 720
    226 Ile Cys Cys Leu Thr Asn Val Thr Ser Thr Ser Leu Val Ser Leu 240
    721 TAC TTC ACT AGA CAG TCC ACC TCT TGC CAC CGT TCG ACC CGA GGG 765
    241 Tyr Phe Thr Arg Gln Ser Thr Ser Cys His Arg Ser Thr Arg Gly 255
    766 TGT TCC CTT AGT TAA ACG GTC CTC AAG GCC ATC TAG AAG GGT CGC 810
    256 Cys Ser Leu Ser End Thr Val Leu Lys Ala Ile End Lys Gly Arg 270
    811 ATT GCC TCT TCC TAT TCG TCC TAG AAT TTA AAC CCC GGC TCG TTT 855
    271 Ile Ala Ser Ser Tyr Ser Ser End Asn Leu Asn Pro Gly Ser Phe 285
    856 TCC ACC TGT ACT AGA AGC GTA GCA AGT ATG CTC TGC GTT TAC GAG 900
    286 Ser Thr Cys Thr Arg Ser Val Ala Ser Met Leu Cys Val Tyr Glu 300
    901 ACT TCC TTT AGG CGG TTC ATG AAC CTG GCC GAC CTC GCA CAT ATT 945
    301 Thr Ser Phe Arg Arg Phe Met Asn Leu Ala Asp Leu Ala His Ile 315
    946 TCT AGT AGA GAT TCT AAC TTT TGG TGG TTC CAG ACC ATT TGT ATC 990
    316 Ser Ser Arg Asp Ser Asn Phe Trp Trp Phe Gln Thr Ile Cys Ile 330
    991 TGC TGT AAT AGG CAC TTA GTC TAC CGT ATT ACC ACC GGG CTC CAC 1035
    331 Cys Cys Asn Arg His Leu Val Tyr Arg Ile Thr Thr Gly Leu His 345
    1036 TAT ACC CGT AAC TCT ATG GGT GCC TCC TAC AAG GGG ATC GGG TCT 1080
    346 Tyr Thr Arg Asn Ser Met Gly Ala Ser Tyr Lys Gly Ile Gly Ser 360
    1081 TTA GCT AAC ACC GAT TCA CGT TGT TCC ACC CGT TCG GAC ACT AGA 1125
    361 Leu Ala Asn Thr Asp Ser Arg Cys Ser Thr Arg Ser Asp Thr Arg 375
    1126 CAC GGT GGG TCT ACT ACC TCA GCT ACT GCT TGT TCG GCG CTG GGT 1170
    376 His Gly Gly Ser Thr Thr Ser Ala Thr Ala Cys Ser Ala Leu Gly 390
    1171 GAG CAC GGC TTC GGA GCC TAC ACC GGT TGC GAT AAA AGC TAC CGA 1215
    391 Glu His Gly Phe Gly Ala Tyr Thr Gly Cys Asp Lys Ser Tyr Arg 405
    1216 CGC TAC GGC ATT ACG ACA GGC CAC TTT GCC GGT TCC CGT TCA TAG 1260
    406 Arg Tyr Gly Ile Thr Thr Gly His Phe Ala Gly Ser Arg Ser End 420
    1261 GCC ACC TCA CGC AGG TCA CGT ACC GGT CCT AGA CAC GGT TTC ATC 1305
    421 Ala Thr Ser Arg Arg Ser Arg Thr Gly Pro Arg His Gly Phe Ile 435
    1306 TTC GTC AAG ATA CCA TGC TTT CGG ACG TCT TGT CGG AAT TTT CCC 1350
    436 Phe Val Lys Ile Pro Cys Phe Arg Thr Ser Cys Arg Asn Phe Pro 450
    1351 TTT AGG CCT GGT CAC GAC GGC TGG TGT AAA GCC GTC AAT GGT GCC 1395
    451 Phe Arg Pro Gly His Asp Gly Trp Cys Lys Ala Val Asn Gly Ala 465
    1396 GCT AGC GCC TCC GCC GGT GCC ACC CGG TTC GGG CCC GTT AAC ACC 1440
    466 Ala Ser Ala Ser Ala Gly Ala Thr Arg Phe Gly Pro Val Asn Thr 480
    1441 ACC GGT CAG GCA CGA GGT ACC ACC GGG TTT ACC AGT CAG TGT ACT 1485
    481 Thr Gly Gln Ala Arg Gly Thr Thr Gly Phe Thr Ser Gln Cys Thr 495
    1486 CCG GCG GTA CAG GTT AAC AGT ACG AGT GCC CAT TGC TTA GGC TCC 1530
    496 Pro Ala Val Gln Val Asn Ser Thr Ser Ala His Cys Leu Gly Ser 510
    1531 GCC GGG TCA GAG ACG AGA AAG CGC CGT AGA TGG GTG AGG AGC AAC 1575
    511 Ala Gly Ser Glu Thr Arg Lys Arg Arg Arg Trp Val Arg Ser Asn 525
    1576 TCC TTT ACC AAT AAC CGT CGA AAT TGA AAG CTG CGT AGT ACG TCA 1620
    526 Ser Phe Thr Asn Asn Arg Arg Asn End Lys Leu Arg Ser Thr Ser 540
    1621 GCC CGG ACT TCG ACT ACC CGT TCT ACC TGT AGA ACC TCG GCC CAG 1665
    541 Ala Arg Thr Ser Thr Thr Arg Ser Thr Cys Arg Thr Ser Ala Gln 555
    1666 TCT TTC CTA GCC ACC ACG ACC AAT TAC GGT ACA GCC GTC TTT TCT 1710
    556 Ser Phe Leu Ala Thr Thr Thr Asn Tyr Gly Thr Ala Val Phe Ser 570
    1711 AGT GGA AGG CCG ACA AGT GAT AGG CAG TCG TCT GGT TCC TTC TTG 1755
    571 Ser Gly Arg Pro Thr Ser Asp Arg Gln Ser Ser Gly Ser Phe Leu 585
    1756 CGC TAG TCC TAC TCG CTA CGG CTT TTA ACC GGG ATC TCG TCT CGA 1800
    586 Arg End Ser Tyr Ser Leu Arg Leu Leu Thr Gly Ile Ser Ser Arg 600
    1801 CGT TCC TCT CCC GAC TTT TCC TCC TTA CGT CTT TCG AGG TCG TTC 1845
    601 Arg Ser Ser Pro Asp Phe Ser Ser Leu Arg Leu Ser Arg Ser Phe 615
    1846 GCC TTC TTA CAG TCT TCG TTT TTG ACC GGT TTT TTA CAT TTG TCA 1890
    616 Ala Phe Leu Gln Ser Ser Phe Leu Thr Gly Phe Leu His Leu Ser 630
    1891 AGC TTC TCT TCG TTT TCC AGA CGG GGT TTT TCT TAC TAT GAG GCT 1935
    631 Ser Phe Ser Ser Phe Ser Arg Arg Gly Phe Ser Tyr Tyr Glu Ala 645
    1936 TCT TAC TAA CAG GCT TCT TTC TAA CAG GCT TCT TTC TTA CAG GGT 1980
    646 Ser Tyr End Gln Ala Ser Phe End Gln Ala Ser Phe Leu Gln Gly 660
    1981 TCG TTC TAC TGC TTT AGA GTT TCA CGT CCG TTT ACG TTC TTC GCC 2025
    661 Ser Phe Tyr Cys Phe Arg Val Ser Arg Pro Phe Thr Phe Phe Ala 675
    2026 TTC GAC TTC TTT CCT TCA CGT TTC TTC TCA AAC TTG TCT ACA CGT 2070
    676 Phe Asp Phe Phe Pro Ser Arg Phe Phe Ser Asn Leu Ser Thr Arg 690
    2071 TTA ATC GAC TTC TTT TTT CCT TTC TTC GAC TTT TTA CGT CCT TTC 2115
    691 Leu Ile Asp Phe Phe Phe Pro Phe Phe Asp Phe Leu Arg Pro Phe 705
    2116 GCC TGC TTC TTG CTT CCT TTC TCC TTC GAC TTT TCA CGT CCT TTA 2160
    706 Ala Cys Phe Leu Leu Pro Phe Ser Phe Asp Phe Ser Arg Pro Leu 720
    2161 ACC TTC TTC TAT CCT TTA CGT TTA ACC GAC TTC TCT TCT TTT CCT 2205
    721 Thr Phe Phe Tyr Pro Leu Arg Leu Thr Asp Phe Ser Ser Phe Pro 735
    2206 TAC TTC TTC TTG AAT TCT ATT ATC TTC GTC TTC ACC GGT TTG ACC 2250
    736 Tyr Phe Phe Leu Asn Ser Ile Ile Phe Val Phe Thr Gly Leu Thr 750
    2251 TTC GAC GAC TCT TCC GGT TCG CAA ACT TCC TCC TTC TCT TTT TCT 2295
    751 Phe Asp Asp Ser Ser Gly Ser Gln Thr Ser Ser Phe Ser Phe Ser 765
    2296 TCC TTC TTG ATT ACT TCA CGT TCG TTG CTT TAC TTC GCT TTT TTT 2340
    766 Ser Phe Leu Ile Thr Ser Arg Ser Leu Leu Tyr Phe Ala Phe Phe 780
    2341 TCT CCC TTC TCC GAC TTT CCA CGT TCT CTT TCC TTT CTC TTT CCC 2385
    781 Ser Pro Phe Ser Asp Phe Pro Arg Ser Leu Ser Phe Leu Phe Pro 795
    2386 TCG ATC GAC TTT ACC TTT TGT TTA CCT TTG TCC AAC GCC TCT TTT 2430
    796 Ser Ile Asp Phe Thr Phe Cys Leu Pro Leu Ser Asn Ala Ser Phe 810
    2431 CTT TCT CTT TCT TCC GTC GTC TCT ACA CGT CCT TTT ATC TCC TAC 2475
    811 Leu Ser Leu Ser Ser Val Val Ser Thr Arg Pro Phe Ile Ser Tyr 825
    2476 GTT TTC TTT TTC GGC GCC GTC TTA GCC GAC TAT TCT AAG ACT TTC 2520
    826 Val Phe Phe Phe Gly Ala Val Leu Ala Asp Tyr Ser Lys Thr Phe 840
    2521 GAC ACA CGC TTT TTG ATT TCG TTA GAG AGA GCC TAG GCC TGT TTA 2565
    841 Asp Thr Arg Phe Leu Ile Ser Leu Glu Arg Ala End Ala Cys Leu 855
    2566 GTT TTT TCC CGT TCT TTA TTT CAC TTT CGA TTA CCG TCT AAA TAT 2610
    856 Val Phe Ser Arg Ser Leu Phe His Phe Arg Leu Pro Ser Lys Tyr 870
    2611 ACC TTG TAA AAT TGA AAA TTA TGC CAC AAT GTG TGG CTT AAG GTT 2655
    871 Thr Leu End Asn End Lys Leu Cys His Asn Val Trp Leu Lys Val 885
    2656 TAA TAA TAT ATT TTG ATA ACC TTA AAA AAT TTA AAA 2691
    886 End End Tyr Ile Leu Ile Thr Leu Lys Asn Leu Lys
    Each codon is read as left nucleotide, top nucleotide, right nucleotide
    Each entry is organized as follows:
    The number of occurrences of the codon in the sequence
    Preference of that codon in organism represented by the codon table
    (as a fraction of all codons coding for the same amino acid)
    Three- letter code for the amino acid coded for according to the codon table
    |A C G T |
    -----------------------------
    A |9 9 13 7 |A
    |0,76 0,12 0,04 0,07 |
    |Lys Thr Arg Ile |
    -----------------------------
    A |16 44 9 11 |C
    |0,61 0,43 0,27 0,46 |
    |Asn Thr Ser Ile |
    -----------------------------
    A |10 13 13 6 |G
    |0,24 0,23 0,03 1 |
    |Lys Thr Arg Met |
    -----------------------------
    A |14 19 15 12 |T
    |0,39 0,21 0,13 0,47 |
    |Asn Thr Ser Ile |
    -----------------------------
    C |3 3 9 4 |A
    |0,31 0,2 0,05 0,03 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |11 10 7 7 |C
    |0,48 0,1 0,37 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    C |14 5 10 8 |G
    |0,69 0,55 0,08 0,55 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |7 13 31 12 |T
    |0,52 0,16 0,42 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    G |1 3 4 8 |A
    |0,7 0,22 0,09 0,17 |
    |Glu Ala Gly Val |
    -----------------------------
    G |18 30 9 15 |C
    |0,41 0,25 0,4 0,2 |
    |Asp Ala Gly Val |
    -----------------------------
    G |10 3 12 5 |G
    |0,3 0,34 0,13 0,34 |
    |Glu Ala Gly Val |
    -----------------------------
    G |4 9 31 11 |T
    |0,59 0,19 0,38 0,29 |
    |Asp Ala Gly Val |
    -----------------------------
    T |11 18 4 25 |A
    |0,62 0,12 0,3 0,11 |
    |End Ser End Leu |
    -----------------------------
    T |18 37 15 46 |C
    |0,47 0,17 0,57 0,49 |
    |Tyr Ser Cys Phe |
    -----------------------------
    T |11 23 9 17 |G
    |0,09 0,13 1 0,11 |
    |End Ser Trp Leu |
    -----------------------------
    T |10 47 17 42 |T
    |0,53 0,19 0,43 0,51 |
    |Tyr Ser Cys Phe |
    -----------------------------
    2 transleerimise raam:
    >gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    2 AAA ATA AGC AAA AAC TGT CCA TTG TGA TGT AAA ATA AAA ACA AAG 46
    1 Lys Ile Ser Lys Asn Cys Pro Leu End Cys Lys Ile Lys Thr Lys 15
    47 CCA CAT AGT TTT AAA TAA GTT TTA AGT AGG TAT GCG TTC TTA GGA 91
    16 Pro His Ser Phe Lys End Val Leu Ser Arg Tyr Ala Phe Leu Gly 30
    92 ATC AGG TCC CAG GTG TCA CGG GTA TAC AAG GTG AAC GGA CAA TTT 136
    31 Ile Arg Ser Gln Val Ser Arg Val Tyr Lys Val Asn Gly Gln Phe 45
    137 GGA TTC TAT AGC GAC GAT ACG GTC GTT TAC GTT CTT TCT CTT CTT 181
    46 Gly Phe Tyr Ser Asp Asp Thr Val Val Tyr Val Leu Ser Leu Leu 60
    182 TGG TGG CTT CAA GGC TGA CCT GGC GCC TGG TAA GCG GAG GGT CAA 226
    61 Trp Trp Leu Gln Gly End Pro Gly Ala Trp End Ala Glu Gly Gln 75
    227 CCT AAT GGT CAG CGC TGA CGT CAT GGT TCG TGG ACG GGA CTC ATA 271
    76 Pro Asn Gly Gln Arg End Arg His Gly Ser Trp Thr Gly Leu Ile 90
    272 GGG GGA GTC GTC GTA GTA TAC GTG GTA ACC TGG GAG TAG GTT GTC 316
    91 Gly Gly Val Val Val Val Tyr Val Val Thr Trp Glu End Val Val 105
    317 GGG TCT TTT CGA TAA CCT CGA GTA GGC CCG ACC CTA CGC CCA GCA 361
    106 Gly Ser Phe Arg End Pro Arg Val Gly Pro Thr Leu Arg Pro Ala 120
    362 CGC ATA CTT GAA GAG AGT GCC GAG AGT GCT CAT AGT GAC GGT CTG 406
    121 Arg Ile Leu Glu Glu Ser Ala Glu Ser Ala His Ser Asp Gly Leu 135
    407 TTA TGT CCG CCG AGC ATT CCG GTA GCG GTA CAT GCA CCT CGT TTG 451
    136 Leu Cys Pro Pro Ser Ile Pro Val Ala Val His Ala Pro Arg Leu 150
    452 CCC AGA CGG GTC TTG TAA CCG TTA ACG AGA CCT GTG GTT CCC AGG 496
    151 Pro Arg Arg Val Leu End Pro Leu Thr Arg Pro Val Val Pro Arg 165
    497 TCT CTA GGC CTG CCC GTT TGA CCG GCC ACC GTT ACT ATC CCG ACT 541
    166 Ser Leu Gly Leu Pro Val End Pro Ala Thr Val Thr Ile Pro Thr 180
    542 CTA TCT CGA GTT CTG GCC TCT GTT TCA CTG TAA CTC GTG GTT CTT 586
    181 Leu Ser Arg Val Leu Ala Ser Val Ser Leu End Leu Val Val Leu 195
    587 CCT CTA CCG TCT GTT CTC ATT GTT CCT ATT ATA GAT ACA CCT AAT 631
    196 Pro Leu Pro Ser Val Leu Ile Val Pro Ile Ile Asp Thr Pro Asn 210
    632 AGT CTC CGA TGG AGT TGA CCA GTT TGG TCC TTT AGC CCA TAA ACA 676
    211 Ser Leu Arg Trp Ser End Pro Val Trp Ser Phe Ser Pro End Thr 225
    677 TCT GCT GCC TAA CTA ACG TAA CTA GCA CTT CCT TAG TTT CCC TCT 721
    226 Ser Ala Ala End Leu Thr End Leu Ala Leu Pro End Phe Pro Ser 240
    722 ACT TCA CTA GAC AGT CCA CCT CTT GCC ACC GTT CGA CCC GAG GGT 766
    241 Thr Ser Leu Asp Ser Pro Pro Leu Ala Thr Val Arg Pro Glu Gly 255
    767 GTT CCC TTA GTT AAA CGG TCC TCA AGG CCA TCT AGA AGG GTC GCA 811
    256 Val Pro Leu Val Lys Arg Ser Ser Arg Pro Ser Arg Arg Val Ala 270
    812 TTG CCT CTT CCT ATT CGT CCT AGA ATT TAA ACC CCG GCT CGT TTT 856
    271 Leu Pro Leu Pro Ile Arg Pro Arg Ile End Thr Pro Ala Arg Phe 285
    857 CCA CCT GTA CTA GAA GCG TAG CAA GTA TGC TCT GCG TTT ACG AGA 901
    286 Pro Pro Val Leu Glu Ala End Gln Val Cys Ser Ala Phe Thr Arg 300
    902 CTT CCT TTA GGC GGT TCA TGA ACC TGG CCG ACC TCG CAC ATA TTT 946
    301 Leu Pro Leu Gly Gly Ser End Thr Trp Pro Thr Ser His Ile Phe 315
    947 CTA GTA GAG ATT CTA ACT TTT GGT GGT TCC AGA CCA TTT GTA TCT 991
    316 Leu Val Glu Ile Leu Thr Phe Gly Gly Ser Arg Pro Phe Val Ser 330
    992 GCT GTA ATA GGC ACT TAG TCT ACC GTA TTA CCA CCG GGC TCC ACT 1036
    331 Ala Val Ile Gly Thr End Ser Thr Val Leu Pro Pro Gly Ser Thr 345
    1037 ATA CCC GTA ACT CTA TGG GTG CCT CCT ACA AGG GGA TCG GGT CTT 1081
    346 Ile Pro Val Thr Leu Trp Val Pro Pro Thr Arg Gly Ser Gly Leu 360
    1082 TAG CTA ACA CCG ATT CAC GTT GTT CCA CCC GTT CGG ACA CTA GAC 1126
    361 End Leu Thr Pro Ile His Val Val Pro Pro Val Arg Thr Leu Asp 375
    1127 ACG GTG GGT CTA CTA CCT CAG CTA CTG CTT GTT CGG CGC TGG GTG 1171
    376 Thr Val Gly Leu Leu Pro Gln Leu Leu Leu Val Arg Arg Trp Val 390
    1172 AGC ACG GCT TCG GAG CCT ACA CCG GTT GCG ATA AAA GCT ACC GAC 1216
    391 Ser Thr Ala Ser Glu Pro Thr Pro Val Ala Ile Lys Ala Thr Asp 405
    1217 GCT ACG GCA TTA CGA CAG GCC ACT TTG CCG GTT CCC GTT CAT AGG 1261
    406 Ala Thr Ala Leu Arg Gln Ala Thr Leu Pro Val Pro Val His Arg 420
    1262 CCA CCT CAC GCA GGT CAC GTA CCG GTC CTA GAC ACG GTT TCA TCT 1306
    421 Pro Pro His Ala Gly His Val Pro Val Leu Asp Thr Val Ser Ser 435
    1307 TCG TCA AGA TAC CAT GCT TTC GGA CGT CTT GTC GGA ATT TTC CCT 1351
    436 Ser Ser Arg Tyr His Ala Phe Gly Arg Leu Val Gly Ile Phe Pro 450
    1352 TTA GGC CTG GTC ACG ACG GCT GGT GTA AAG CCG TCA ATG GTG CCG 1396
    451 Leu Gly Leu Val Thr Thr Ala Gly Val Lys Pro Ser Met Val Pro 465
    1397 CTA GCG CCT CCG CCG GTG CCA CCC GGT TCG GGC CCG TTA ACA CCA 1441
    466 Leu Ala Pro Pro Pro Val Pro Pro Gly Ser Gly Pro Leu Thr Pro 480
    1442 CCG GTC AGG CAC GAG GTA CCA CCG GGT TTA CCA GTC AGT GTA CTC 1486
    481 Pro Val Arg His Glu Val Pro Pro Gly Leu Pro Val Ser Val Leu 495
    1487 CGG CGG TAC AGG TTA ACA GTA CGA GTG CCC ATT GCT TAG GCT CCG 1531
    496 Arg Arg Tyr Arg Leu Thr Val Arg Val Pro Ile Ala End Ala Pro 510
    1532 CCG GGT CAG AGA CGA GAA AGC GCC GTA GAT GGG TGA GGA GCA ACT 1576
    511 Pro Gly Gln Arg Arg Glu Ser Ala Val Asp Gly End Gly Ala Thr 525
    1577 CCT TTA CCA ATA ACC GTC GAA ATT GAA AGC TGC GTA GTA CGT CAG 1621
    526 Pro Leu Pro Ile Thr Val Glu Ile Glu Ser Cys Val Val Arg Gln 540
    1622 CCC GGA CTT CGA CTA CCC GTT CTA CCT GTA GAA CCT CGG CCC AGT 1666
    541 Pro Gly Leu Arg Leu Pro Val Leu Pro Val Glu Pro Arg Pro Ser 555
    1667 CTT TCC TAG CCA CCA CGA CCA ATT ACG GTA CAG CCG TCT TTT CTA 1711
    556 Leu Ser End Pro Pro Arg Pro Ile Thr Val Gln Pro Ser Phe Leu 570
    1712 GTG GAA GGC CGA CAA GTG ATA GGC AGT CGT CTG GTT CCT TCT TGC 1756
    571 Val Glu Gly Arg Gln Val Ile Gly Ser Arg Leu Val Pro Ser Cys 585
    1757 GCT AGT CCT ACT CGC TAC GGC TTT TAA CCG GGA TCT CGT CTC GAC 1801
    586 Ala Ser Pro Thr Arg Tyr Gly Phe End Pro Gly Ser Arg Leu Asp 600
    1802 GTT CCT CTC CCG ACT TTT CCT CCT TAC GTC TTT CGA GGT CGT TCG 1846
    601 Val Pro Leu Pro Thr Phe Pro Pro Tyr Val Phe Arg Gly Arg Ser 615
    1847 CCT TCT TAC AGT CTT CGT TTT TGA CCG GTT TTT TAC ATT TGT CAA 1891
    616 Pro Ser Tyr Ser Leu Arg Phe End Pro Val Phe Tyr Ile Cys Gln 630
    1892 GCT TCT CTT CGT TTT CCA GAC GGG GTT TTT CTT ACT ATG AGG CTT 1936
    631 Ala Ser Leu Arg Phe Pro Asp Gly Val Phe Leu Thr Met Arg Leu 645
    1937 CTT ACT AAC AGG CTT CTT TCT AAC AGG CTT CTT TCT TAC AGG GTT 1981
    646 Leu Thr Asn Arg Leu Leu Ser Asn Arg Leu Leu Ser Tyr Arg Val 660
    1982 CGT TCT ACT GCT TTA GAG TTT CAC GTC CGT TTA CGT TCT TCG CCT 2026
    661 Arg Ser Thr Ala Leu Glu Phe His Val Arg Leu Arg Ser Ser Pro 675
    2027 TCG ACT TCT TTC CTT CAC GTT TCT TCT CAA ACT TGT CTA CAC GTT 2071
    676 Ser Thr Ser Phe Leu His Val Ser Ser Gln Thr Cys Leu His Val 690
    2072 TAA TCG ACT TCT TTT TTC CTT TCT TCG ACT TTT TAC GTC CTT TCG 2116
    691 End Ser Thr Ser Phe Phe Leu Ser Ser Thr Phe Tyr Val Leu Ser 705
    2117 CCT GCT TCT TGC TTC CTT TCT CCT TCG ACT TTT CAC GTC CTT TAA 2161
    706 Pro Ala Ser Cys Phe Leu Ser Pro Ser Thr Phe His Val Leu End 720
    2162 CCT TCT TCT ATC CTT TAC GTT TAA CCG ACT TCT CTT CTT TTC CTT 2206
    721 Pro Ser Ser Ile Leu Tyr Val End Pro Thr Ser Leu Leu Phe Leu 735
    2207 ACT TCT TCT TGA ATT CTA TTA TCT TCG TCT TCA CCG GTT TGA CCT 2251
    736 Thr Ser Ser End Ile Leu Leu Ser Ser Ser Ser Pro Val End Pro 750
    2252 TCG ACG ACT CTT CCG GTT CGC AAA CTT CCT CCT TCT CTT TTT CTT 2296
    751 Ser Thr Thr Leu Pro Val Arg Lys Leu Pro Pro Ser Leu Phe Leu 765
    2297 CCT TCT TGA TTA CTT CAC GTT CGT TGC TTT ACT TCG CTT TTT TTT 2341
    766 Pro Ser End Leu Leu His Val Arg Cys Phe Thr Ser Leu Phe Phe 780
    2342 CTC CCT TCT CCG ACT TTC CAC GTT CTC TTT CCT TTC TCT TTC CCT 2386
    781 Leu Pro Ser Pro Thr Phe His Val Leu Phe Pro Phe Ser Phe Pro 795
    2387 CGA TCG ACT TTA CCT TTT GTT TAC CTT TGT CCA ACG CCT CTT TTC 2431
    796 Arg Ser Thr Leu Pro Phe Val Tyr Leu Cys Pro Thr Pro Leu Phe 810
    2432 TTT CTC TTT CTT CCG TCG TCT CTA CAC GTC CTT TTA TCT CCT ACG 2476
    811 Phe Leu Phe Leu Pro Ser Ser Leu His Val Leu Leu Ser Pro Thr 825
    2477 TTT TCT TTT TCG GCG CCG TCT TAG CCG ACT ATT CTA AGA CTT TCG 2521
    826 Phe Ser Phe Ser Ala Pro Ser End Pro Thr Ile Leu Arg Leu Ser 840
    2522 ACA CAC GCT TTT TGA TTT CGT TAG AGA GAG CCT AGG CCT GTT TAG 2566
    841 Thr His Ala Phe End Phe Arg End Arg Glu Pro Arg Pro Val End 855
    2567 TTT TTT CCC GTT CTT TAT TTC ACT TTC GAT TAC CGT CTA AAT ATA 2611
    856 Phe Phe Pro Val Leu Tyr Phe Thr Phe Asp Tyr Arg Leu Asn Ile 870
    2612 CCT TGT AAA ATT GAA AAT TAT GCC ACA ATG TGT GGC TTA AGG TTT 2656
    871 Pro Cys Lys Ile Glu Asn Tyr Ala Thr Met Cys Gly Leu Arg Phe 885
    2657 AAT AAT ATA TTT TGA TAA CCT TAA AAA ATT TAA AAG 2692
    886 Asn Asn Ile Phe End End Pro End Lys Ile End Lys
    Each codon is read as left nucleotide, top nucleotide, right nucleotide
    Each entry is organized as follows:
    The number of occurrences of the codon in the sequence
    Preference of that codon in organism represented by the codon table
    (as a fraction of all codons coding for the same amino acid)
    Three-letter code for the amino acid coded for according to the codon table
    |A C G T |
    -----------------------------
    A |10 11 10 13 |A
    |0,76 0,12 0,04 0,07 |
    |Lys Thr Arg Ile |
    -----------------------------
    A |4 10 7 3 |C
    |0,61 0,43 0,27 0,46 |
    |Asn Thr Ser Ile |
    -----------------------------
    A |4 15 15 3 |G
    |0,24 0,23 0,03 1 |
    |Lys Thr Arg Met |
    -----------------------------
    A |6 27 13 16 |T
    |0,39 0,21 0,13 0,47 |
    |Asn Thr Ser Ile |
    -----------------------------
    C |7 22 13 26 |A
    |0,31 0,2 0,05 0,03 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |13 15 6 10 |C
    |0,48 0,1 0,37 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    C |7 35 8 7 |G
    |0,69 0,55 0,08 0,55 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |6 49 17 45 |T
    |0,52 0,16 0,42 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    G |8 7 11 24 |A
    |0,7 0,22 0,09 0,17 |
    |Glu Ala Gly Val |
    -----------------------------
    G |8 9 13 17 |C
    |0,41 0,25 0,4 0,2 |
    |Asp Ala Gly Val |
    -----------------------------
    G |10 8 4 13 |G
    |0,3 0,34 0,13 0,34 |
    |Glu Ala Gly Val |
    -----------------------------
    G |4 16 16 40 |T
    |0,59 0,19 0,38 0,29 |
    |Asp Ala Gly Val |
    -----------------------------
    T |16 9 13 20 |A
    |0,62 0,12 0,3 0,11 |
    |End Ser End Leu |
    -----------------------------
    T |14 6 5 14 |C
    |0,47 0,17 0,57 0,49 |
    |Tyr Ser Cys Phe |
    -----------------------------
    T |10 20 10 5 |G
    |0,09 0,13 1 0,11 |
    |End Ser Trp Leu |
    -----------------------------
    T |4 44 8 38 |T
    |0,53 0,19 0,43 0,51 |
    |Tyr Ser Cys Phe |
    -----------------------------
    3 transleerimise raam:
    >gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    3 AAA TAA GCA AAA ACT GTC CAT TGT GAT GTA AAA TAA AAA CAA AGC 47
    0 Lys End Ala Lys Thr Val His Cys Asp Val Lys End Lys Gln Ser 14
    48 CAC ATA GTT TTA AAT AAG TTT TAA GTA GGT ATG CGT TCT TAG GAA 92
    15 His Ile Val Leu Asn Lys Phe End Val Gly Met Arg Ser End Glu 29
    93 TCA GGT CCC AGG TGT CAC GGG TAT ACA AGG TGA ACG GAC AAT TTG 137
    30 Ser Gly Pro Arg Cys His Gly Tyr Thr Arg End Thr Asp Asn Leu 44
    138 GAT TCT ATA GCG ACG ATA CGG TCG TTT ACG TTC TTT CTC TTC TTT 182
    45 Asp Ser Ile Ala Thr Ile Arg Ser Phe Thr Phe Phe Leu Phe Phe 59
    183 GGT GGC TTC AAG GCT GAC CTG GCG CCT GGT AAG CGG AGG GTC AAC 227
    60 Gly Gly Phe Lys Ala Asp Leu Ala Pro Gly Lys Arg Arg Val Asn 74
    228 CTA ATG GTC AGC GCT GAC GTC ATG GTT CGT GGA CGG GAC TCA TAG 272
    75 Leu Met Val Ser Ala Asp Val Met Val Arg Gly Arg Asp Ser End 89
    273 GGG GAG TCG TCG TAG TAT ACG TGG TAA CCT GGG AGT AGG TTG TCG 317
    90 Gly Glu Ser Ser End Tyr Thr Trp End Pro Gly Ser Arg Leu Ser 104
    318 GGT CTT TTC GAT AAC CTC GAG TAG GCC CGA CCC TAC GCC CAG CAC 362
    105 Gly Leu Phe Asp Asn Leu Glu End Ala Arg Pro Tyr Ala Gln His 119
    363 GCA TAC TTG AAG AGA GTG CCG AGA GTG CTC ATA GTG ACG GTC TGT 407
    120 Ala Tyr Leu Lys Arg Val Pro Arg Val Leu Ile Val Thr Val Cys 134
    408 TAT GTC CGC CGA GCA TTC CGG TAG CGG TAC ATG CAC CTC GTT TGC 452
    135 Tyr Val Arg Arg Ala Phe Arg End Arg Tyr Met His Leu Val Cys 149
    453 CCA GAC GGG TCT TGT AAC CGT TAA CGA GAC CTG TGG TTC CCA GGT 497
    150 Pro Asp Gly Ser Cys Asn Arg End Arg Asp Leu Trp Phe Pro Gly 164
    498 CTC TAG GCC TGC CCG TTT GAC CGG CCA CCG TTA CTA TCC CGA CTC 542
    165 Leu End Ala Cys Pro Phe Asp Arg Pro Pro Leu Leu Ser Arg Leu 179
    543 TAT CTC GAG TTC TGG CCT CTG TTT CAC TGT AAC TCG TGG TTC TTC 587
    180 Tyr Leu Glu Phe Trp Pro Leu Phe His Cys Asn Ser Trp Phe Phe 194
    588 CTC TAC CGT CTG TTC TCA TTG TTC CTA TTA TAG ATA CAC CTA ATA 632
    195 Leu Tyr Arg Leu Phe Ser Leu Phe Leu Leu End Ile His Leu Ile 209
    633 GTC TCC GAT GGA GTT GAC CAG TTT GGT CCT TTA GCC CAT AAA CAT 677
    210 Val Ser Asp Gly Val Asp Gln Phe Gly Pro Leu Ala His Lys His 224
    678 CTG CTG CCT AAC TAA CGT AAC TAG CAC TTC CTT AGT TTC CCT CTA 722
    225 Leu Leu Pro Asn End Arg Asn End His Phe Leu Ser Phe Pro Leu 239
    723 CTT CAC TAG ACA GTC CAC CTC TTG CCA CCG TTC GAC CCG AGG GTG 767
    240 Leu His End Thr Val His Leu Leu Pro Pro Phe Asp Pro Arg Val 254
    768 TTC CCT TAG TTA AAC GGT CCT CAA GGC CAT CTA GAA GGG TCG CAT 812
    255 Phe Pro End Leu Asn Gly Pro Gln Gly His Leu Glu Gly Ser His 269
    813 TGC CTC TTC CTA TTC GTC CTA GAA TTT AAA CCC CGG CTC GTT TTC 857
    270 Cys Leu Phe Leu Phe Val Leu Glu Phe Lys Pro Arg Leu Val Phe 284
    858 CAC CTG TAC TAG AAG CGT AGC AAG TAT GCT CTG CGT TTA CGA GAC 902
    285 His Leu Tyr End Lys Arg Ser Lys Tyr Ala Leu Arg Leu Arg Asp 299
    903 TTC CTT TAG GCG GTT CAT GAA CCT GGC CGA CCT CGC ACA TAT TTC 947
    300 Phe Leu End Ala Val His Glu Pro Gly Arg Pro Arg Thr Tyr Phe 314
    948 TAG TAG AGA TTC TAA CTT TTG GTG GTT CCA GAC CAT TTG TAT CTG 992
    315 End End Arg Phe End Leu Leu Val Val Pro Asp His Leu Tyr Leu 329
    993 CTG TAA TAG GCA CTT AGT CTA CCG TAT TAC CAC CGG GCT CCA CTA 1037
    330 Leu End End Ala Leu Ser Leu Pro Tyr Tyr His Arg Ala Pro Leu 344
    1038 TAC CCG TAA CTC TAT GGG TGC CTC CTA CAA GGG GAT CGG GTC TTT 1082
    345 Tyr Pro End Leu Tyr Gly Cys Leu Leu Gln Gly Asp Arg Val Phe 359
    1083 AGC TAA CAC CGA TTC ACG TTG TTC CAC CCG TTC GGA CAC TAG ACA 1127
    360 Ser End His Arg Phe Thr Leu Phe His Pro Phe Gly His End Thr 374
    1128 CGG TGG GTC TAC TAC CTC AGC TAC TGC TTG TTC GGC GCT GGG TGA 1172
    375 Arg Trp Val Tyr Tyr Leu Ser Tyr Cys Leu Phe Gly Ala Gly End 389
    1173 GCA CGG CTT CGG AGC CTA CAC CGG TTG CGA TAA AAG CTA CCG ACG 1217
    390 Ala Arg Leu Arg Ser Leu His Arg Leu Arg End Lys Leu Pro Thr 404
    1218 CTA CGG CAT TAC GAC AGG CCA CTT TGC CGG TTC CCG TTC ATA GGC 1262
    405 Leu Arg His Tyr Asp Arg Pro Leu Cys Arg Phe Pro Phe Ile Gly 419
    1263 CAC CTC ACG CAG GTC ACG TAC CGG TCC TAG ACA CGG TTT CAT CTT 1307
    420 His Leu Thr Gln Val Thr Tyr Arg Ser End Thr Arg Phe His Leu 434
    1308 CGT CAA GAT ACC ATG CTT TCG GAC GTC TTG TCG GAA TTT TCC CTT 1352
    435 Arg Gln Asp Thr Met Leu Ser Asp Val Leu Ser Glu Phe Ser Leu 449
    1353 TAG GCC TGG TCA CGA CGG CTG GTG TAA AGC CGT CAA TGG TGC CGC 1397
    450 End Ala Trp Ser Arg Arg Leu Val End Ser Arg Gln Trp Cys Arg 464
    1398 TAG CGC CTC CGC CGG TGC CAC CCG GTT CGG GCC CGT TAA CAC CAC 1442
    465 End Arg Leu Arg Arg Cys His Pro Val Arg Ala Arg End His His 479
    1443 CGG TCA GGC ACG AGG TAC CAC CGG GTT TAC CAG TCA GTG TAC TCC 1487
    480 Arg Ser Gly Thr Arg Tyr His Arg Val Tyr Gln Ser Val Tyr Ser 494
    1488 GGC GGT ACA GGT TAA CAG TAC GAG TGC CCA TTG CTT AGG CTC CGC 1532
    495 Gly Gly Thr Gly End Gln Tyr Glu Cys Pro Leu Leu Arg Leu Arg 509
    1533 CGG GTC AGA GAC GAG AAA GCG CCG TAG ATG GGT GAG GAG CAA CTC 1577
    510 Arg Val Arg Asp Glu Lys Ala Pro End Met Gly Glu Glu Gln Leu 524
    1578 CTT TAC CAA TAA CCG TCG AAA TTG AAA GCT GCG TAG TAC GTC AGC 1622
    525 Leu Tyr Gln End Pro Ser Lys Leu Lys Ala Ala End Tyr Val Ser 539
    1623 CCG GAC TTC GAC TAC CCG TTC TAC CTG TAG AAC CTC GGC CCA GTC 1667
    540 Pro Asp Phe Asp Tyr Pro Phe Tyr Leu End Asn Leu Gly Pro Val 554
    1668 TTT CCT AGC CAC CAC GAC CAA TTA CGG TAC AGC CGT CTT TTC TAG 1712
    555 Phe Pro Ser His His Asp Gln Leu Arg Tyr Ser Arg Leu Phe End 569
    1713 TGG AAG GCC GAC AAG TGA TAG GCA GTC GTC TGG TTC CTT CTT GCG 1757
    570 Trp Lys Ala Asp Lys End End Ala Val Val Trp Phe Leu Leu Ala 584
    1758 CTA GTC CTA CTC GCT ACG GCT TTT AAC CGG GAT CTC GTC TCG ACG 1802
    585 Leu Val Leu Leu Ala Thr Ala Phe Asn Arg Asp Leu Val Ser Thr 599
    1803 TTC CTC TCC CGA CTT TTC CTC CTT ACG TCT TTC GAG GTC GTT CGC 1847
    600 Phe Leu Ser Arg Leu Phe Leu Leu Thr Ser Phe Glu Val Val Arg 614
    1848 CTT CTT ACA GTC TTC GTT TTT GAC CGG TTT TTT ACA TTT GTC AAG 1892
    615 Leu Leu Thr Val Phe Val Phe Asp Arg Phe Phe Thr Phe Val Lys 629
    1893 CTT CTC TTC GTT TTC CAG ACG GGG TTT TTC TTA CTA TGA GGC TTC 1937
    630 Leu Leu Phe Val Phe Gln Thr Gly Phe Phe Leu Leu End Gly Phe 644
    1938 TTA CTA ACA GGC TTC TTT CTA ACA GGC TTC TTT CTT ACA GGG TTC 1982
    645 Leu Leu Thr Gly Phe Phe Leu Thr Gly Phe Phe Leu Thr Gly Phe 659
    1983 GTT CTA CTG CTT TAG AGT TTC ACG TCC GTT TAC GTT CTT CGC CTT 2027
    660 Val Leu Leu Leu End Ser Phe Thr Ser Val Tyr Val Leu Arg Leu 674
    2028 CGA CTT CTT TCC TTC ACG TTT CTT CTC AAA CTT GTC TAC ACG TTT 2072
    675 Arg Leu Leu Ser Phe Thr Phe Leu Leu Lys Leu Val Tyr Thr Phe 689
    2073 AAT CGA CTT CTT TTT TCC TTT CTT CGA CTT TTT ACG TCC TTT CGC 2117
    690 Asn Arg Leu Leu Phe Ser Phe Leu Arg Leu Phe Thr Ser Phe Arg 704
    2118 CTG CTT CTT GCT TCC TTT CTC CTT CGA CTT TTC ACG TCC TTT AAC 2162
    705 Leu Leu Leu Ala Ser Phe Leu Leu Arg Leu Phe Thr Ser Phe Asn 719
    2163 CTT CTT CTA TCC TTT ACG TTT AAC CGA CTT CTC TTC TTT TCC TTA 2207
    720 Leu Leu Leu Ser Phe Thr Phe Asn Arg Leu Leu Phe Phe Ser Leu 734
    2208 CTT CTT CTT GAA TTC TAT TAT CTT CGT CTT CAC CGG TTT GAC CTT 2252
    735 Leu Leu Leu Glu Phe Tyr Tyr Leu Arg Leu His Arg Phe Asp Leu 749
    2253 CGA CGA CTC TTC CGG TTC GCA AAC TTC CTC CTT CTC TTT TTC TTC 2297
    750 Arg Arg Leu Phe Arg Phe Ala Asn Phe Leu Leu Leu Phe Phe Phe 764
    2298 CTT CTT GAT TAC TTC ACG TTC GTT GCT TTA CTT CGC TTT TTT TTC 2342
    765 Leu Leu Asp Tyr Phe Thr Phe Val Ala Leu Leu Arg Phe Phe Phe 779
    2343 TCC CTT CTC CGA CTT TCC ACG TTC TCT TTC CTT TCT CTT TCC CTC 2387
    780 Ser Leu Leu Arg Leu Ser Thr Phe Ser Phe Leu Ser Leu Ser Leu 794
    2388 GAT CGA CTT TAC CTT TTG TTT ACC TTT GTC CAA CGC CTC TTT TCT 2432
    795 Asp Arg Leu Tyr Leu Leu Phe Thr Phe Val Gln Arg Leu Phe Ser 809
    2433 TTC TCT TTC TTC CGT CGT CTC TAC ACG TCC TTT TAT CTC CTA CGT 2477
    810 Phe Ser Phe Phe Arg Arg Leu Tyr Thr Ser Phe Tyr Leu Leu Arg 824
    2478 TTT CTT TTT CGG CGC CGT CTT AGC CGA CTA TTC TAA GAC TTT CGA 2522
    825 Phe Leu Phe Arg Arg Arg Leu Ser Arg Leu Phe End Asp Phe Arg 839
    2523 CAC ACG CTT TTT GAT TTC GTT AGA GAG AGC CTA GGC CTG TTT AGT 2567
    840 His Thr Leu Phe Asp Phe Val Arg Glu Ser Leu Gly Leu Phe Ser 854
    2568 TTT TTC CCG TTC TTT ATT TCA CTT TCG ATT ACC GTC TAA ATA TAC 2612
    855 Phe Phe Pro Phe Phe Ile Ser Leu Ser Ile Thr Val End Ile Tyr 869
    2613 CTT GTA AAA TTG AAA ATT ATG CCA CAA TGT GTG GCT TAA GGT TTA 2657
    870 Leu Val Lys Leu Lys Ile Met Pro Gln Cys Val Ala End Gly Leu 884
    2658 ATA ATA TAT TTT GAT AAC CTT AAA AAA TTT AAA AGA 2693
    885 Ile Ile Tyr Phe Asp Asn Leu Lys Lys Phe Lys Arg
    Each codon is read as left nucleotide, top nucleotide, right nucleotide
    Each entry is organized as follows:
    The number of occurrences of the codon in the sequence
    Preference of that codon in organism represented by the codon table
    (as a fraction of all codons coding for the same amino acid)
    Three-letter code for the amino acid coded for according to the codon table
    |A C G T |
    -----------------------------
    A |15 11 6 10 |A
    |0,76 0,12 0,04 0,07 |
    |Lys Thr Arg Ile |
    -----------------------------
    A |13 3 12 0 |C
    |0,61 0,43 0,27 0,46 |
    |Asn Thr Ser Ile |
    -----------------------------
    A |10 24 8 7 |G
    |0,24 0,23 0,03 1 |
    |Lys Thr Arg Met |
    -----------------------------
    A |3 1 5 3 |T
    |0,39 0,21 0,13 0,47 |
    |Asn Thr Ser Ile |
    -----------------------------
    C |10 10 21 24 |A
    |0,31 0,2 0,05 0,03 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |24 3 12 35 |C
    |0,48 0,1 0,37 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    C |6 16 29 15 |G
    |0,69 0,55 0,08 0,55 |
    |Gln Pro Arg Leu |
    -----------------------------
    C |9 11 16 62 |T
    |0,52 0,16 0,42 0,1 |
    |His Pro Arg Leu |
    -----------------------------
    G |6 7 3 3 |A
    |0,7 0,22 0,09 0,17 |
    |Glu Ala Gly Val |
    -----------------------------
    G |21 7 12 26 |C
    |0,41 0,25 0,4 0,2 |
    |Asp Ala Gly Val |
    -----------------------------
    G |9 6 10 8 |G
    |0,3 0,34 0,13 0,34 |
    |Glu Ala Gly Val |
    -----------------------------
    G |11 11 12 17 |T
    |0,59 0,19 0,38 0,29 |
    |Asp Ala Gly Val |
    -----------------------------
    T |18 7 4 12 |A
    |0,62 0,12 0,3 0,11 |
    |End Ser End Leu |
    -----------------------------
    T |27 18 9 64 |C
    |0,47 0,17 0,57 0,49 |
    |Tyr Ser Cys Phe |
    -----------------------------
    T |25 11 9 15 |G
    |0,09 0,13 1 0,11 |
    |End Ser Trp Leu |
    -----------------------------
    T |13 8 6 48 |T
    |0,53 0,19 0,43 0,51 |
    |Tyr Ser Cys Phe |
    -----------------------------
    Aminohapete sisaldus:
    Protein: gi|24648965|ref|NM_142773.1| Drosophila melanogaster CG7069-RA (CG7069), mRNA
    Length = 2693 amino acids
    Molecular Weight = 233938,92 Daltons
    Amino Acid Number Mol%
    Ala A 519 19,27
    Cys C 743 27,59
    Asp D 0 0,00
    Glu E 0 0,00
    Phe F 0 0,00
    Gly G 551 20,46
    His H 0 0,00
    Ile I 0 0,00
    Lys K 0 0,00
    Leu L 0 0,00
    Met M 0 0,00
    Asn N 0 0,00
    Pro P 0 0,00
    Gln Q 0 0,00
    Arg R 0 0,00
    Ser S 0 0,00
    Thr T 880 32,68
    Val V 0 0,00
    Trp W 0 0,00
    Tyr Y 0 0,00
    Hüdrofoobsusprofiil:
    Hüdrofiilsusprofiil:
  • Vasakule Paremale
    BIOinformaatika kodutöö 5 #1 BIOinformaatika kodutöö 5 #2 BIOinformaatika kodutöö 5 #3 BIOinformaatika kodutöö 5 #4 BIOinformaatika kodutöö 5 #5 BIOinformaatika kodutöö 5 #6 BIOinformaatika kodutöö 5 #7 BIOinformaatika kodutöö 5 #8 BIOinformaatika kodutöö 5 #9 BIOinformaatika kodutöö 5 #10 BIOinformaatika kodutöö 5 #11 BIOinformaatika kodutöö 5 #12 BIOinformaatika kodutöö 5 #13 BIOinformaatika kodutöö 5 #14 BIOinformaatika kodutöö 5 #15 BIOinformaatika kodutöö 5 #16 BIOinformaatika kodutöö 5 #17 BIOinformaatika kodutöö 5 #18 BIOinformaatika kodutöö 5 #19 BIOinformaatika kodutöö 5 #20 BIOinformaatika kodutöö 5 #21 BIOinformaatika kodutöö 5 #22 BIOinformaatika kodutöö 5 #23 BIOinformaatika kodutöö 5 #24 BIOinformaatika kodutöö 5 #25 BIOinformaatika kodutöö 5 #26 BIOinformaatika kodutöö 5 #27 BIOinformaatika kodutöö 5 #28 BIOinformaatika kodutöö 5 #29 BIOinformaatika kodutöö 5 #30 BIOinformaatika kodutöö 5 #31 BIOinformaatika kodutöö 5 #32 BIOinformaatika kodutöö 5 #33 BIOinformaatika kodutöö 5 #34 BIOinformaatika kodutöö 5 #35 BIOinformaatika kodutöö 5 #36 BIOinformaatika kodutöö 5 #37 BIOinformaatika kodutöö 5 #38 BIOinformaatika kodutöö 5 #39 BIOinformaatika kodutöö 5 #40 BIOinformaatika kodutöö 5 #41 BIOinformaatika kodutöö 5 #42 BIOinformaatika kodutöö 5 #43 BIOinformaatika kodutöö 5 #44 BIOinformaatika kodutöö 5 #45 BIOinformaatika kodutöö 5 #46 BIOinformaatika kodutöö 5 #47 BIOinformaatika kodutöö 5 #48 BIOinformaatika kodutöö 5 #49 BIOinformaatika kodutöö 5 #50 BIOinformaatika kodutöö 5 #51 BIOinformaatika kodutöö 5 #52 BIOinformaatika kodutöö 5 #53 BIOinformaatika kodutöö 5 #54 BIOinformaatika kodutöö 5 #55 BIOinformaatika kodutöö 5 #56 BIOinformaatika kodutöö 5 #57 BIOinformaatika kodutöö 5 #58 BIOinformaatika kodutöö 5 #59 BIOinformaatika kodutöö 5 #60 BIOinformaatika kodutöö 5 #61 BIOinformaatika kodutöö 5 #62 BIOinformaatika kodutöö 5 #63 BIOinformaatika kodutöö 5 #64 BIOinformaatika kodutöö 5 #65 BIOinformaatika kodutöö 5 #66 BIOinformaatika kodutöö 5 #67 BIOinformaatika kodutöö 5 #68 BIOinformaatika kodutöö 5 #69 BIOinformaatika kodutöö 5 #70 BIOinformaatika kodutöö 5 #71 BIOinformaatika kodutöö 5 #72 BIOinformaatika kodutöö 5 #73 BIOinformaatika kodutöö 5 #74 BIOinformaatika kodutöö 5 #75 BIOinformaatika kodutöö 5 #76 BIOinformaatika kodutöö 5 #77 BIOinformaatika kodutöö 5 #78 BIOinformaatika kodutöö 5 #79
    Punktid 100 punkti Autor soovib selle materjali allalaadimise eest saada 100 punkti.
    Leheküljed ~ 79 lehte Lehekülgede arv dokumendis
    Aeg2012-11-01 Kuupäev, millal dokument üles laeti
    Allalaadimisi 46 laadimist Kokku alla laetud
    Kommentaarid 0 arvamust Teiste kasutajate poolt lisatud kommentaarid
    Autor Mumm Õppematerjali autor
    Viienda kodutöö ülesannete vastused aines bioinformaatikaAndmebaasid (KEGG, NCBI, RCSB), järjestuste (SMS, BioEdit) ja struktuuride (SPdbV) analüüs

    Kasutatud allikad

    Sarnased õppematerjalid

    Geeni ekspressioon-translatsioon ja geneetiline kood
    34
    ppt

    Geeni ekspressioon: translatsioon ja geneetiline kood

    Geeni ekspressioon: translatsioon ja geneetiline kood Geeni väljendus on valk; kui rakul on vaja valku, siis tuleb lugeda vastavat geneetilist koodi. 1. Translatsioon mRNAl oleva info konverteerimine a/h järjestuseks ehk polüpeptiidiks Geneetilise info lugemine kahe etapiline: 1. Transkriptsioon ­ sünteesitakse DNA matriitsil üheahelaline RNA molekul DNA matriitsil Valk ehk proteiin: Suure mol.kaaluga, lämmastikku sisaldav orgaaniline ühend Sisaldab ühte või mitut polüpeptiidi. Polüpeptiidid koosnevad aminohapetest Aminohapped: Amino grupp (NH2) Karboksüül grupp (COOH) Tüüpiiselt on laetud kaks rühma a/h (NH3+ and COO) Vesiniku aatom R grupp (igal a/h erinev) 20 erinevat aminohapet: Lühendid kolmetähelised vahel ka ühetähelised Neli gruppi (omadused R grupist): 1. Happelisi (n = 2) 2. Aluselisi (n = 3) 3. Ne

    Geneetika
    Molekulaarbioloogia konspekt
    38
    pdf

    Molekulaarbioloogia konspekt

    1 Õppevahend: Molekulaarbioloogia üldkursuse lühikonspekt Põhiline õpik on B. Lewin "Genes" V ja VI väljaanne, edasises tekstis on viiteid Genes VI joonistele (kui pole eraldi märgitud) ja üksikutel teemadel detailsematele materjalidele. Kursiivis on esitatud lõigud, mis on mõeldud täindavaks lugemiseks aga ei ole "kohustuslikud". Sissejuhatus Molekulaarbioloogia on termin, mis võeti kasutusele selle sajandi teisel poolel peale esimeste makromolekulide ruumilise struktuuri kindlakstegemist. Esialgu tähistaski see termin just struktuurset bioloogiat molekulaarsel tasemel. Seega on molekulaarbioloogia oma algses tähenduses keemia ja füüsika meetodeid kasutav bioloogia osa, mis tegeleb bioloogiliste makromolekulide ruumilise struktuuri ja struktuuri ning funktsiooni vaheliste seoste kindlakstegemisega. Hiljem, kui sama teaduse piirid on ähmas

    Molekulaarbioloogia
    Liha töötlemine
    1168
    pdf

    Liha töötlemine

    Handbook of Meat Processing Handbook of Meat Processing Fidel Toldrá EDITOR A John Wiley & Sons, Inc., Publication Edition first published 2010 © 2010 Blackwell Publishing Blackwell Publishing was acquired by John Wiley & Sons in February 2007. Blackwell’s publishing program has been merged with Wiley’s global Scientific, Technical, and Medical business to form Wiley-Blackwell. Editorial Office 2121 State Avenue, Ames, Iowa 50014-8300, USA For details of our global editorial offices, for customer services, and for information about how to apply for permission to reuse the copyright material in this book, please see our website at www.wiley.com/ wiley-blackwell. Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Blackwell Publishing, provided that the base fee is paid directly to the Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923. F

    Inglise keel
    Lühendite seletus
    120
    doc

    Lühendite seletus

    A... AA Auto Answer AAA Authentication, Authorization and Accounting AAB All-to-All Broadcast AAC Advanced Audio Coding AACS Advanced Access Control System AAL Asynchronous Transfer Mode Adaption Layer AAM Automatic Acoustic Management AAP Applications Access Point [DEC] AARP AppleTalk Address Resolution Protocol AAS All-to-All Scatter AASP ASCII Asynchronous Support Package AAT Average Access Time AATP Authorized Academic Training Program [Microsoft] .ABA Address Book Archive (file name extension) [Palm] ABAP Advanced Business Application Programming [SAP] ABC * Atanasoff-Berry Computer (First digital calculating machine that used vacuum tubes) ABEND Abnormal End ABI Application Binary Interface ABIOS Advanced BIOS ABIST Automatic Built-In Self-Test [IBM] ABLE Adaptive Battery Life Extender + Agent Building and Learning Environment [IBM] ABM Asynchronous Balanced Mode ABR Available Bit Rate ABRD

    Informaatika



    Meedia

    Kommentaarid (0)

    Kommentaarid sellele materjalile puuduvad. Ole esimene ja kommenteeri



    Sellel veebilehel kasutatakse küpsiseid. Kasutamist jätkates nõustute küpsiste ja veebilehe üldtingimustega Nõustun