Kuidas muutub LDH v11 over-expression mutandis etanooli tootlikkus õrreldes wild-type tüvega kui laktaadi tootmine v12 kasvab 2x kuid ülejäänud mõõdetud vood jäävad samaks?

Vastus leiad õppematerjalist: Kodutöö biotehnoloogias

Kodutöö biotehnoloogias (0)

Tallinna Tehnikaülikool - Bioloogia - Biotehnoloogia

1 Hindamata

Esitatud küsimused

Kuidas muutub LDH v11 over-expression mutandis etanooli tootlikkus õrreldes wild-type tüvega kui laktaadi tootmine v12 kasvab 2x kuid ülejäänud mõõdetud vood jäävad samaks?

Overview

Sheet1
Sheet2
Sheet3

Sheet 1: Sheet1

Kodutöö biotehnoloogias

Sheet 2: Sheet2

v1 v2 v3 v4 v5 b1 b2 b3 b4
A -1 0 0 0 0 1 0 0 0
B 2 -2 0 0 0 0 0 0 0
C 0 2 -2 1 0 0 -1 0 0
D 0 0 1 0 1 0 0 -1 0
E 0 0 0 0 1 0 0 0 0
F 0 0 0 -2 -2 0 0 0 1
Mõõdetud
b1 25
b2 6
b4 5
STÖHHIOMEETRILINE MAATRIKS A
MÕÕDETUD VOOGUDE STÖHHIOMEETRILINE MAATRIKS
v1 v2 v3 v4 v5 b3
b1 b2 b4 A -1 0 0 0 0 0
A 1 0 0
B 2 -2 0 0 0 0
B 0 0 0
C 0 2 -2 1 0 0
C 0 -1 0
D 0 0 1 0 1 -1
D 0 0 0
E 0 0 0 0 1 0
E 0 0 0
F 0 0 0 -2 -2 0
F 0 0 1
PÖÖRDMAATRIKS
TASAKAALUVEKTOR b
TUNDMATUD VOOD
v1 v2 v3 v4 v5 b3
-25
25
A -1 0 0 0 0 0
0
25
B -1 -0.5 0 0 0 0
6
23.25
C -1 -0.5 -0.5 0 -0.5 -0.25
0
2.5
D 0 0 0 0 -1 -0.5
0
0
E 0 0 0 0 1 0
-5
23.25
F -1 -0.5 -0.5 -1 0.5 -0.25

Sheet 3: Sheet3

V1 V2 V3 V4 V5 V6 V7 V8 V9 V10 V11 V12 V13 V14 V15 V16 V17 V18 V19 V20 V21 V22 V23 V24 V25 V26 V27 V28 V29 V30 O1 O2 O3 O4 O5 O6 O7 O8 O9 O10 O11
1 G6P 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0
2 F6P 0 1 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 0 0 0 0 0 0
3 FBP 0 0 1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4 GAP 0 0 0 0 1 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 -1 0 0 0 0 0 0
5 Dihydroxyacetone-P 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
6 GBP 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7 3PG 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0 0
8 PEP 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0
9 PYR 0 0 0 0 0 0 0 0 0 1 -1 0 -1 0 0 -1 0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0
10 Lactate 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11 Ace-CoA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0
12 Acetyl-P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
13 Acetate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
14 Acetaldehyde 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
15 Ethanol 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
16 Formate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
17 OXA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0
18 2KG 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1
19 Gluc-1,5-lactone-6P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
20 6P-Gluconate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
21 Ru-5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0
22 R5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 -1 0 0 0 0 0 0 0 0
23 X5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0
24 S7P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0
25 E4P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 -1 0 0 0 0 0 0 0
V1 V2 V3 V5 V7 V8 V9 V10 V12 V13 V14 V15 V16 V17 V18 V19 V22 V23 V24 V25 V26 V27 V28 V29 V30
V4 V6 V11 V20 V21 O1 O2 O3 O4 O5 O6 O7 O8 O9 O10 O11
1 G6P 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0 0 0
0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0
2 F6P 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
1 0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0
3 FBP 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
-1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4 GAP 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1
0 1 0 0 0 0 0 0 0 -1 0 0 0 0 0 0
5 Dihydroxyacetone-P 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
6 GBP 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7 3PG 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0 0
8 PEP 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 -1 0 0 0 0
9 PYR 0 0 0 0 0 0 0 1 0 -1 0 0 -1 0 0 0 -1 0 0 0 0 0 0 0 0
0 0 -1 0 0 0 0 0 0 0 0 0 -1 0 0 0
10 Lactate 0 0 0 0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
11 Ace-CoA 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 -1 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0 0
12 Acetyl-P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
13 Acetate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0 0
14 Acetaldehyde 0 0 0 0 0 0 0 0 0 1 -1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
15 Ethanol 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
16 Formate 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0
17 OXA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 0
18 2KG 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1
19 Gluc-1,5-lactone-6P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
20 6P-Gluconate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
21 Ru-5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 -1 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
22 R5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0
0 0 0 0 0 0 0 -1 0 0 0 0 0 0 0 0
23 X5P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
24 S7P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
25 E4P 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
0 0 0 0 0 0 0 0 -1 0 0 0 0 0 0 0
Inverse
1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 2 1
0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1
0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
0 0 0 -1 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
0 0 0 -1 1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
0 0 0 -1 1 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
0 0 0 0 0 0 0 0 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 -1 1 -1 -1 -1 -1 0 0 0 0 0 0 -1 -1 -1 0 0 0 0 0 1 0
0 0 0 -1 1 -1 -1 -1 -1 0 -1 -1 -1 -1 0 0 -1 -1 0 0 0 0 0 1 0
0 0 0 -1 1 -1 -1 -1 -1 0 -1 -1 -1 -1 -1 0 -1 -1 0 0 0 0 0 1 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 -1 -1 -1 0 0 1 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 2 2
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 2
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 2
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
v0)kuidas muutub LDH (v11) over-expression mutandis etanooli tootlikkus
Known fluxes
Balance
Calculated fluxes
võrreldes wild -type tüvega, kui laktaadi tootmine (v12) kasvab 2x, kuid ülejäänud mõõdetud vood jäävad samaks?
V4 10
-80
V1 450
V12 50
-60
V2 10
Known fluxes
Balance
Calculated fluxes
Etanooli tootlikkus wild- types
0.3043478261
V7 750
-10
V3 60
V4 10
-80
V1 500
Etanooli tootlikkus mutandis
0.2739130435
V20 50
-50
V5 50
V12 100
-60
V2 60
V21 35
-50
V6 0
V7 750
-10
V3 110
O1 80
0
V15 0
V20 50
0
V5 100
O2 70
-130
V8 -130
V21 35
-100
V6 100
M(ethanole)=46g/mol
O3 140
-160
V9 -290
O1 80
0
V15 100
O4 110
-790
V10 750
O2 70
-130
V8 -30
O5 100
750
V11 - 1230
O3 140
-160
V9 -190
Yps=(V16/M)/(V1/M)
O6 130
-60
V13 - 1305
O4 110
-790
V10 750
O7 160
0
V14 -1305
O5 100
750
V11 - 1130
O8 40
-50
V16 35
O6 130
-60
V13 - 1205
O9 60
0
V17 -75
O7 160
0
V14 -1205
O10 20
0
V18 50
O8 40
-50
V16 35
O11 95
-35
V19 50
O9 60
0
V17 -75
-20
V22 115
O10 20
0
V18 50
-95
V23 95
O11 95
-35
V19 50
0
V24 360
-20
V22 115
0
V25 360
-95
V23 95
0
V26 360
0
V24 360
-140
V27 250
0
V25 360
0
V28 110
0
V26 360
0
V29 110
-140
V27 250
-110
V30 110
0
V28 110
0
V29 110
-110
V30 110

.XLSX Laadi alla originaalfail 25 lk · .xlsx · 5 allalaadimist

10 punkti Autor soovib selle materjali allalaadimise eest saada 10 punkti.

~ 25 lehte Lehekülgede arv dokumendis

2016-01-11 Kuupäev, millal dokument üles laeti

5 laadimist Kokku alla laetud

0 arvamust Teiste kasutajate poolt lisatud kommentaarid

ilmanimeta Õppematerjali autor

Üliõpilaskoodi lõpp 0

etanooli tootlikkus maatriks biotehnoloogia bioloogia

Sarnased õppematerjalid

297

pdf

THE PSYCHOLOGY OF COMMUNICATION

W. Lambert Gardiner has been leading his life in neat, The Psychology of Communications multiple-of-five-year installments for the convenience of biographers. VOLUME 1 1935-1955 GROWING IN SCOTLAND Flunked out of elementary school, High School, and Glasgow University. The Psychology of VOLUME 2 1955-1960 STUDYING IN CANADA Communication Work by day and study by night. B. A. Sir George Williams University. High School Teaching Diploma McGill University. VOLUME 3 1960-1965 STUDYING IN USA Ph. D. Cornell University. Nothing else happened. VOLUME 6 1980-1985 VOLUME 4 1965-1970

Psüholoogia

571

doc

Mikolaj Kopernik

#;h_èMZ-C}#v#R^#&#*;Y9`0#? #SVrM6+#1nM#Z3j1##Kv? #P^###ocQEz0#qq#z4?Um? #a#z##[#[##J%#J@ ##GI_- k#G Z t%d #S##jRc#mg# 3#m#|s<|#ATW#:6c *[` # [X #<#Q##> 4mT~*i6#- - ,u#U#Ayrmb#44lq#x#ZQml#d##{ :uZG3r?S#T0l-c#n U%y#%]90# zw[*wV1Q####n##c4$r##Xy.APio*E## #s I#wN#x>j=5Yr5O#^4 ;#}#Mahi%[8,GR- _6mx-U#y#y!d3h&?u.-,'#'- `8Vvoq#}3Km4h2O6Nv<- 9/w+FkF"+! R2#R#dOuc#Gi9[#s# #V#MQB#]#S##O7u#wnV 8'#:#m($#:| Q?}su[## P~<#g7#kAj#Kj^/#$U#JR X$Kx ? p#~4+7(} QY#V U?y# Y#p? AYHv.QMt_##Y<$14 g[J#/3Q- z"#? [#!6~T##in#9 #Oj+X0_UN~##*]7)@? ###?K}B#5S aEF#@#{ ## FsTyc[ T `8=O5ny#N##&t&####M# L~DZC2I#M%Vw#fo##aM,`+##i- m##=8 o@,n1e#o3X- ~, $n)#n##)PN^v@nNO8'5Z+##nDw b#vy$|^.TM;#Li N#o##'? o.##N

Füüsika

1168

pdf

Liha töötlemine

Handbook of Meat Processing Handbook of Meat Processing Fidel Toldrá EDITOR A John Wiley & Sons, Inc., Publication Edition first published 2010 © 2010 Blackwell Publishing Blackwell Publishing was acquired by John Wiley & Sons in February 2007. Blackwell’s publishing program has been merged with Wiley’s global Scientific, Technical, and Medical business to form Wiley-Blackwell. Editorial Office 2121 State Avenue, Ames, Iowa 50014-8300, USA For details of our global editorial offices, for customer services, and for information about how to apply for permission to reuse the copyright material in this book, please see our website at www.wiley.com/ wiley-blackwell. Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Blackwell Publishing, provided that the base fee is paid directly to the Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923. F

Inglise keel