The most commonly used capillaries with a length of 50 to 75 cm. As a rule, another 515 cm between the detector and the tank to drain is adding. 21 Consitioning One of the most important factors of good reproducibility CE is capillary conditioning. Formation of reproducible surface of the capillary is one of the biggest problems in HECE. Conditions were more reproducible if the capillary contacts only with the buffer. However, in practice this is not possible due to the adsorption of sample components and changing the EOF. More often, to delete adsorption products on surface, conditioning by alkalis is applied
electroporation, a rapid pulse of high-voltage direct current. Once inside the cell, the DNA is integrated and the foreign gene will express. These two techniques largely depend upon the development of protoplast systems that retain the capacity to regenerate intact plants. Transgenic corn, rice, and soybean have been produced with these techniques, especially electroporation. Success rates, however, are low, and the techniques not very reproducible. DNA can also be microinjected into target plant cells using very thin glass needles in a method similar to that used with animals. Microinjection, however, has produced only a few transgenic plants. The technique is laborious, technically difficult, and limited to the number of cells actually injected. Biolistics, involves accelerating very small particles of tungsten or gold coated with DNA into cells using an electrostatic pulse, air pressure, or gunpowder percussion. As
Meta-analüüsid. Replikatsioonid. Süstemaatilised replikatsioonid sh "reproducibility project". Eelregistreeritud uuringud ("pre- registered studies") publitseerimisnihke vältimiseks: uurijad saadavad ajakirjale uuringuprotokolli (eksperimendi vm meetodi täpse kirjelduse), retsensentide positiivse hinnangu korral garanteeritakse selle uuringu põhjal kirjutatud artikli avaldamine olenemata sellest, kas tulemus on "positiivne" või mitte. "Reproducible research" (andmete, uuringuprotokollide, analüüsimeetodite avaldamine). Uuringutüübid: eksperiment, pseudoeksperiment, läbilõikeuuring (cross-sectional study), longituuduuring, "kultuuri- taseme" andmed (epidemioloogias kutsutakse seda "ökoloogiliseks" uuringuks), tekstianalüüs jne. Isiksuseomaduste mõõtmine: - Sotsiaalne taju (sh enese- ja teistekohased andmed) • - Käitumise vaatlused • Implitsiitsed hoiakud •
adequate results from phylogenetic [3] or phylogeographic to distinguish between individuals by compiling a specific profile analysis [4]. Therefore it is crucial to distinguish between real for each individual analysed. Comparing these profiles can help us informative allelic variants and the misleading single mutated to assess which specimens are similar enough on a genetic level to copies, to achieve reproducible findings. For this reason, a small be used together for one analysis e.g. for separating an enzyme of number of cloned sequences may not suffice to describe the whole interest or compiling a cDNA library. complement of the genome and there is no guarantee that these few sequences are actually the most prominent and true Materials and Methods descriptors for the individual studied. To overcome this problem,
Kuidas tagada uuringutulemuste korratavus? Meta-analüüsid. Replikatsioonid. Süstemaatilised replikatsioonid sh "reproducibility project". Eelregistreeritud uuringud ("pre-registered studies") publitseerimisnihke vältimiseks: uurijad saadavad ajakirjale uuringuprotokolli (eksperimendi vm meetodi täpse kirjelduse), retsensentide positiivse hinnangu korral garanteeritakse selle uuringu põhjal kirjutatud artikli avaldamine olenemata sellest, kas tulemus on "positiivne" või mitte. "Reproducible research" (andmete, uuringuprotokollide, analüüsimeetodite avaldamine). Uuringutüübid: eksperiment, pseudoeksperiment, läbilõikeuuring (cross-sectional study), longituuduuring, "kultuuri-taseme" andmed (epidemioloogias kutsutakse seda "ökoloogiliseks" uuringuks), tekstianalüüs jne. Infoallikad isiksuse kohta - igapäevane käitumine - reputatsioon: üldine arvamus, kuulujututd - teiste hinnangud - enesekohased väited - kujutlusvõime produktid - reaktsioonid - eluloolised andmed
, psychrotrophs 3 Dogbevi et al. 1999 Novel Technologies for Microbial Spoilage Prevention 267 Several advantages of phage technology otic cells. To our knowledge, sensory data for meat spoilage control are described by demonstrating that phage treatments do not others (Greer 2005; Hudson et al. 2005). For alter the appearance or flavor of foods have example, phages are self-reproducible and not been published. release more phage after bacterial lysis. Among the many potential drawbacks of Phage specificity may be an advantage if phage technology, limited host range is con- selective for spoilage microflora only. On the sidered most important. Most reported studies other hand, specificity may diminish phage were performed on experimentally inocu- activity against broad-spectrum spoilage lated refrigerated meats and demonstrated
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