5 Wheat Grass Nisuoras +33.8 14 Juurviljad White Raddish (Spring) Jaapani redis, kevadine korje +3.1 Rutabaga Kaalikas +3.1 Kohlrabi Nuikapsas +5.1 Horseradish Mädarõigas +6.8 Turnip Naeris +8.0 Carrot Porgand +9.5 Beet Peet, puna- +11.3 Red Radish Redis +16.7
Abingdon, UK). Emotion regulation in relation.. 1 2.3.2. Detection of Salivary Cortisol Salivary cortisol was dectected using a commercially available ELISA (Salimetrics, LCC, USA). Briefly, a 96-well microtitre plate coated with monoclonal antibodies to cortisol was used. Cortisol standards (3.000, 1.000, 0.333, 0.111, 0.037, and 0.012 g/ml) and the unknown compete with cortisol linked to horseradish perxidase for the antibody binding sites. Following incubation (55 mins) the plate was washed to remove unbound material. Bound cortisol peroxidase was measured by the reaction of the peroxidase enzyme on the substrate tetramethylbenzidine (TMB). After incubation for 25 mins this reaction was stopped with 50 l per well of stop solution (H 2SO4). The colour change was measured on a plate reader at 450 nm. The cortisol peroxidase
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found that oregano essential oil extract storage at 3°C. extended shelf life of refrigerated MAP- We believe essential oils and plant extracts stored fresh meat. probably have limited application for shelf Allyl isothiocyanate is one of many vola- life extension of fresh meats due to flavor tile natural antimicrobials found in cruci- changes associated with the quantities needed ferous plants, such as horseradish, black to achieve meaningful results. On the other mustard, cabbage, and turnip. Nadarajah et hand, in applications where flavoring is al. (2005a) prepared paper disks containing expected, such as with seasoned or marinated 1 ml of 65% allyl isothiocyanate mixed with products, use of essential oils may be benefi- corn oil. They then applied the paper disks to cial to extend shelf life. ground beef patties that were then vacuum
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