E/V 1.0 3.0 5.0 7.0 9.0 11.0 13.0 15.0 17.0 0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 NaOH V, ml Graafikute põhjal võib järeldada, et esimene ekvivalentpunkt on 5,5 ml ning teine ekvivalentpunkt 12,5 ml NaOH lisamisel, sest seal on kõige suuremad tõusud. Vastavalt Skoog 2017 Appendix3 fosforhappe dissotsiatsioonikonstandid on 25oC juures järgmised: K1 =7,11·10-3 K2 = 6,32 ·10-8 K3 = 4,5·10-13 Valemid: 1) Ioonmeeter: pHalg ¿ 3,0 H +¿=10-3,0=0,001(vesinikioonide kontsentratsioon ) +¿ pH H , seega C ¿ ¿-log C ¿ H +¿ C¿ ¿ ¿2 , kus Ka1 =7,11·10-3 ¿ ¿ Chape =¿ ( 0,001 )2 C ( H 3 PO 4 )= -3
järvi- ja Merikalan Helsinki 2004 34. EE, 1995 35. "Kalastaja" 2001, Nr.22 36. http://www.mi.mun.ca/mi-net/enviro/organ.htm 37. Roots,O;Järv,L ;Simm,M "DDT and PCB concentrations dependency on the biology and domicile of fish: an example of Perch (Perca fluviatilis L.) in Estonian coastal Sea" ; 2004 38. Läänemereportaal, 2008, http://www.fimr.fi/et/et_EE/et/ 39. OÜ Eesti Keskkonnauuringute Keskus "Ohtlike ainete Seireprogrammi uuendamine" 2007 40. Skoog D.A., Leary J.J. Principles of Instrumental Analysis. Fourth edition, Saunders College 1992 47 LISA 1 LISA 2
protoplasts formed; growth on nutrient medium; plant growth regulators / named growth regulator; R hormones rooting; incubation in light; plantlets; subdivide; handling, medium / sterile soil; AVP; AVP; e.g. remove wax from leaves callus culture / mass of undifferentiated cells forms ref. auxin to cytokinin ratio Murashige and Skoog (M & S) medium further detail of culture method / aseptic technique max 5 (c) max 4 for either advantages many plants; genetically identical; (so) all have desired, characteristics / genotypes / phenotypes; no need for (artificial) selection; can be obtained in short space of time / AW; easy to, transport / store; A ref to space saving easy to genetically engineer; disease / virus, free;
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