9. Strategies to reduce hepatitis C virus recurrence after liver transplantation Ruben Ciria, María Pleguezuelo, Shirin Elizabeth Khorsandi et.al https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664282/ (07.05.2018) 10. Joonis 1: The molecular and structural basis of advanced antiviral therapy for hepatitis C virus infection Ralf Bartenschlage, Volker Lohmann and Francois Penin http://www.nature.com.ezproxy.utlib.ut.ee/articles/nrmicro3046.pdf (07.05.2018) 11. Oligonucleotide-Lipid Conjugates Forming G-Quadruplex Structures Are Potent and Pangenotypic Hepatitis C Virus Entry Inhibitors In Vitro and Ex Vivo, George Koutsoudakis, Alexia Paris de León, Carolina Herrera et.al https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404530/ (07.05.2018) 12. Ledipasvir and Sofosbuvir for Previously Treated HCV Genotype 1 Infection, Nezam Afdhal, M.D., K. Rajender Reddy, M.D., David R. Nelson, M.D https://www.nejm.org/doi/full/10
FRET (fluoresence resonance energy transfere) tehnika -Iga SNP vajab spetsiaalpraimereid -Kõige efektiivsemad väheste SNP-de ja suure hulga proovide puhul Introduction to TaqMan® probes TaqMan® probes are dual labeled hydrolysis probes and are a registered trademark of the Roche Molecular Systems, Inc. TaqMan® probes utilize the 5' exonuclease activity of the enzyme Taq Polymerase for measuring the amount of target sequences in the samples. TaqMan® probes consist of a 18- 22 bp oligonucleotide probe which is labeled with a reporter fluorophore at the 5' end and a quencher fluorophore at the 3' end. TaqMan® probe functioning While carrying out a TaqMan® experiment, a fluorogenic probe, complementary to the target sequence is added to the PCR reaction mixture. This probe is an oligonucleotide with a reporter dye attached to the 5' end and a quencher dye attached to the 3' end. Till the time the probe is not hydrolized, the quencher and
Quantification is et al. 1997). only possible, therefore, at the beginning of The “sequence-specific fluorescent the exponential phase when the reaction probes” can be classified into two major is totally efficient and all the reagents are groups: hydrolysis and hybridization probes. available. The most important parameter in Both types consist of an oligonucleotide, the RTi-PCR is the threshold cycle (CT value) homologous to the internal region of the (Higuchi et al. 1992), which is used for the amplicon, that is double-labeled, with a fluo- quantification of the sample. It corresponds rophore or reporter dye (donor of fluores- to the cycle at which a statistically significant cence) at the 5′-end and a quenching moiety increase in amplification-associated flores- (acceptor of fluorescence) at the 3′-end
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