Artikli autor(id). Aasta. Artikli pealkiri. Ajakirja pealkiri lühendatult. Köite number, ajakirja number, leheküljenumbrid. [viide meediale] sidusandmebaas (kontrollimise kuupäev) . Karofeld, E. 1998. Effects of bombing and regeneration of plant cover in Kõnnu-Suursoo raised bog, North Estonia. Wetl. Ecol. Manage, 6, 4, 253-260. [Online] Web of Science (19.02.2007) Karofeld, E., Pajula, R. 2005. Distribution and development of necrotic Sphagnum patches in two Estonian raised bogs. Folia Geobotanica. [e-journal] (http://www.tlu.ee/~foliageob- foorum/) (19.01.2007) Muu allikas internetis: Autor(id). Aasta kui võimalik. Pealkiri. [viide meediale] URL aadress (kontrollimise kuupäev): Mets, K. Keemia õpetamisest keskkoolis 20. sajandi lõpus. [WWW] http://www.tlu.ee/keemia/matrejalid/opetamine.html (14.02.2007) Digitaalsel andmekandjal (CD, DVD vms) olev andmebaas: Autor(id). Aasta. Pealkiri
residues and are brought into the cell via endocytosis. Once inside the cell protonation of the amines results in an influx of counter-ions and a lowering of the osmotic potential. Osmotic swelling results and bursts the vesicle releasing the polymer-DNA complex (polyplex) into the cytoplasm. If the polyplex unpacks then the DNA is free to diffuse to the nucleus.] PEI is extremely cytotoxic, by two different mechanisms, the disruption of the cell membrane leading to necrotic cell death (immediate) and disruption of the mitochondrial membrane after internalisation leading to apoptosis (delayed). Esialgu tuleb vaadelda rakkud mikroskoobis ja hinnata nende seisukorra ja tiheduse. Rakud ei ole saastanud ega surnud. Rakkude tihedus on umbes 90%. Transfektsioonisegu ettevalmistamine: Kõigepealt tuleb lahjendada seerumvabas rakusöötmes (DMEM) eraldi epsides DNA ja PEI.
, X. Vignon, and M. Bonnet. 1991. Osmotic 371:171–176. pressure changes in postmortem muscles: Factors of Sorimachi, H., and K. Suzuki. 2001. The structure of variation and possible causative agents. Proceedings calpain. Journal of Biochemistry 129:653–664. of the 37th International Congress of Meat Science Spencer, M. J., D. E. Croall, and J. G. Tidball. 1995. and Technology 452–456. Calpains are activated in necrotic fibers from mdx Ouali, A., C. H. Herrera-Mendez, G. Coulis, S. Becila, dystrophic mice. Journal of Biological Chemistry A. Boudjellal, L. Aubry, and M. A. Sentandreu. 2006. 270:10909–10914. 104 Chapter 4 Stevenson, B. J., J. D. Morton, M. A. Ilian, and R. and solubility. Journal of Animal Science Bickerstaffe. 2002. Effect of muscle stretching on titin 72:1209–1223. and calpain 3: Implication for tenderness. Proceedings Van Laack, R. L., C
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