proliferisatsiooniks vajalikud substansid. Antibiootikumid-et bakterid vohama ei hakkaks, meie rakud olid antibiootikumile resistentsed (vist). Aminohapped- loomarakk ei ole võimeline kõiki ah ise sünteesima. · Milline on seerumi keskkond, kas seerum on puhverdatud lahus? Seerumi keskkond võiks olla neutraalselähedane (pH 7,2- 7,4) Puhverdatus küsitav.... Serum- watery fluid of the blood that resembles plasma but contains fibrinogen. · Millised tingimused on vajalikud loomarakkude kasvatamiseks kultuuris, miks nad peavad olema just sellised? Loomsed rakud vajavad rikastatud söödet: essentsiaalsed aminohapped (Lys, His, Leu, Ile, Met, Phe, Thr, Trp, Val; Cys, Glu, Tyr), Kasvufaktoreid, Vitamiine, Seerumit,(Kinnitus maatriksit (ECM kinnituseks) koosneb kollageenist, hüaluroon happest ja teistest ECM valkudest). Osad võivad kasvada ka 3D geelis ja suspensioonis.
and surimi; egg white powder; raw egg white; and Seguro 1998; De Jong and Koppelman bovine, porcine, lamb, and broiler plasma 2002). powders; and gelatine (Chen and Trout 1991; Fibrimex (Harimex Inc., Alberta, Canada) Lu and Chen 1999). The major drawback of is a blood-based cold binding system that the hot-set method is that the product must binds meat pieces based on the blood clotting be marketed pre-cooked and/or frozen. action between fibrinogen and thrombin, Several cold-set techniques have been resulting in the conversion of fibrinogen to developed to meet the demand for restruc- fibrin which cross-link with collagen and gel, tured meats that can be sold in the raw chilled thereby binding the meat pieces being state (Clarke et al. 1988; Esguerra 1994; restructured (Boles and Shand 1998). Nielsen et al. 1996). Alginate, a polysaccha- Pearl Meat cold-set binders are a carbohy-
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