Lahtrites vasakust paremale : 1. Marker 2. BSA (Mw = 66,5 kDA) 3. Trypsin (Mw =23,8 kDA) 4. Lactate dehüdrogenase ( Mw = 35 kDA) 5. Tundmatu 6. Albumin (Ovalbumin) (Mw = 44,3 kDa) 7. Proteinase K ( Mw = 28,5 kDa) Migreerumistee Mw Valk pikkus (cm) (kDA) BSA 2.9 66.5 Albumine 4.6 44.3 Lactate dehydrogenase 5.2 35 Proteinase K 5.7 28.5 Trypsin 6.5 23.8 Tundmatu 6.2 ? Geeli pikkus (cm) 7.4 Kalibreerimisgraafik : Y=Mw X=migreerimispikkus Y= -12.351*6.2 + 101.13 = 24.55 Vastus: meie valgu kontsentratsioon on 24.6 kDa
Substances that have a circadian variation (e.g., cortisol) can be properly interpreted only with knowledge of the time of day the sample was drawn. During specimen collection, other principles should be remembered. Specimens should not be drawn above an intravenous line, as this may contaminate the sample with intravenous fluid. Lysis of cells during collection of a blood specimen will result in spuriously increased serum levels of substances concentrated in cells (e.g., lactate dehydrogenase and potassium). Certain test specimens may require special handling or storage (e.g., blood gas specimens). Delay in delivery of specimens to the laboratory can result in ongoing cellular metabolism and therefore spurious results for some studies (e.g., low blood glucose). Conclusion Obtaining a proper medical history, performing a complete physical examination, and having a thorough understanding of the accuracy, precision, sensitivity, specificity, and indications of
fosfataas) on kasutatud maksakahjustuse näitajana juba 1920ndatest. Hüdrolüüsib mitmeid fosfaadi estreid. AP on glükoproteiin. Võib leiduda eelkõige maksas, soolestikus, neerudes, luudes. kreatiini kinaas- (creatine kinase- CK) katalüüsib pöörduvat kreatiini fosforüülimist, et moodustada kreatiini fosfaate. bilirubiin- omab suhteliselt väikest diagnostilist tähtsust. Kõrge bilirubiini tase veres võib viia kolaltõveni. sorbitooldehüdrogenaas- (sorbitol dehydrogenase- SDH) pöörduvalt oksüdeerib p-sorbitooli n-fruktoosiks koos kofaktor NAD'ga (nikotiinamiid adeniin dinukleotiid). Hobuse ja veise seerum kaotab 25% SDH aktiivsusest külmutamisel. glutamaatdehüdrogenaas- (glutamate dehydrogenase-GDH) NAD on kofaktioriks glutamaadi dehüdrogenaasi reaktsioonis, tootmiseks alfa-ketoglutaraati ja ammooniumi. laktaatdehüdrogenaas- (lactate dehüdrogenaas- LDH) Koos kofaktori NAD'ga katalüüsib pöörduva püruvaadi oksidatsiooni r-(f)-laktaadiks. 12
demethylation of N-, S-, and O-methyl compounds. After oxidation of formaldehyde to formate, the carbon atom is further oxidized to carbon dioxide (CO2) or incorporated into purines, thymidine, and amino acids via tetrahydrofolatedependent one-carbon biosynthetic pathways. Exogenous formaldehyde appears to be readily absorbed from the respiratory and gastrointestinal tracts, but poorly absorbed following dermal application. Formaldehyde is metabolized to formate by the enzyme formaldehyde dehydrogenase; this appears to take place at the initial site of contact. Being normal components of intermediary metabolism, neither formaldehyde nor formate are stored to any significant extent in any tissue of the body. Formate is excreted in the urine (primarily as formic acid), incorporated into other cellular molecules, or oxidized to carbon dioxide and exhaled. Formaldehyde vapors are readily absorbed from the respiratory tract. Due to rapid metabolism to
Taimed Vastus: gi|1351271|sp|P48496|TPIC_SPIOL Triosephosphate isomerase, chloroplast precursor (TIM) (Triose-phosphate isomerase) 211 9e-54 vii. Selgroogsed Vastus: gi|6678413|ref|NP_033441.1| triosephosphate isomerase 1 [Mus musculus] 195 2e-48 viii. Putukad Vastus: gi|22090453|emb|CAD43178.1| triosephosphate isomerase [Tenebrio molitor] 213 2e-54 ix. Kalad Vastus: gi|62125774|gb|AAX63800.1| NADH dehydrogenase subunit 5 [Myxocyprinus asiaticus] 23.9 2.3 x. Linnud Vastus: gi|45382061|ref|NP_990782.1| triosephosphate isomerase 1 [Gallus gallus] 192 9e-49 xi. Imetajad Vastus: gi|6678413|ref|NP_033441.1| triosephosphate isomerase 1 [Mus musculus] 195 2e-48 xii. Primaadid Vastus: gi|83674984|gb|ABC40672.1| rcTPI1 [Gorilla gorilla] 192 4e-48 f
" "Ummm ... Okay." "You'd have trouble finishing a 10K." I nodded. "In fact, I think you'd have trouble finishing a 5K." This wasn't exactly what I wanted to hear, but three months of obsessing over ultraendurance had taken me many places, and this was one of them: enzymes. CS, 3HAD, LDH, and PFK are all enzymes that limit energy production through di erent pathways. South African Xhosa mid- and long-distance runners, for example, have high levels of lactate dehydrogenase (LDH), which allows them to recycle lactate at higher than normal rates. More LDH appears to mean less accumulation of plasma lactate (more commonly referred to as "lactic acid"), which means less debilitating muscle burn. In Kenyan runners, higher levels of another enzyme, 3-HAD, mean a greater ability to use fat instead of carbohydrate during sub- maximal exercise. How did I measure up? I am the one under the line. Hear me roar:
annaabi.ee 
