ning erinevalt antibiootikumidest, ei mõjuta faagid ning nende produktid eukarüootseid rakke. Seega kõrvalmõjusid esineb faagiteraapias vähem kui antibiootikumidega ravimisel (Matsuzaki, 2005). Bakteriofaagide genoomide modifitseerimine Bakteriofaagide antimikroobsete omaduste võimendamiseks on välja töötatud erinevaid tehnikaid. Hiljuti loodi uus in vivo tehnika: bakteriofaagi elektroporeeritud DNA rekombineerimine ehk BRED (bacteriophage recombineering of electroporated DNA). BRED töötati välja Mycobacterium’i faagide jaoks, kuid seda saab rakendada ka teiste faagide peal. BRED-i puhul viiakse elektroporatsiooni abil faagi DNA bakteriraku sisse ning plasmiidis olevad rekombinatsiooni geenid üleekspresseeritakse, et tõsta homoloogilise rekombinatsiooni sagedust faagi DNA ja sihtmärk DNA vahel. BRED-i läbiviimiseks kasutatakse enamasti faag λ- t või Rac profaagi
Fleischmann, R.D., Adams,M.D., White, O., Clayton, R.A., Kirkness, E.F., Kerlavage, A.R., Bult, C.J., Tomb, J.F., Dougherty, B.A., Merrick, J.M., et al. (1995). Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269 (5223): 496-512. International Human Genome Sequencing Consortium. (2004). Finishing the euchromatic sequence of the human genome. Nature 431: 931-945. Godson, G.N, Barrell, B.G., Staden, R., Fiddes, J.C. (1978). Nucleotide sequence of bacteriophage G4 DNA. Nature 276: 236-247. Gritsenko, A.A, Nijkamp, J.F., Reinders, M.J.T, de Ridder, D. (2012). Bioinformatics 28(11): 1429-37. Narzisi G., Mishra, B. (2011). Comparing de novo genome assembly: the long and short of it. PLoS One. 6 (4). Wu, W., Stupi, B.P., Litosh, V.A., Mansouri, D., Farley, D., Morris, S., Metzker, S., Metzker, M.L. (2007). Termination of DNA synthesis by N6-alkylated, not 3-O-alkylated, photocleavable 2-deoxyadenosine triphosphates
Phage specificity may be an advantage if phage technology, limited host range is con- selective for spoilage microflora only. On the sidered most important. Most reported studies other hand, specificity may diminish phage were performed on experimentally inocu- activity against broad-spectrum spoilage lated refrigerated meats and demonstrated microflora. Whitman and Marshall (1971a) efficacy against spoilage psychrotrophs and noticed that phages from bacteriophage-host psychrophiles (Greer 1986, 1988; Greer and systems isolated from refrigerated food prod- Dilts 2002; Greer et al. 2007). Greer (1988) ucts usually attacked only those hosts upon showed that homologous phage can replicate which they were isolated. Phages are gener- and limit bacterial growth on inoculated and ally more stable than their hosts and can refrigerated phage-treated beef. Maximum survive processing (Koo et al. 2000)
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