ref to flow rate ; size of beads ; A number / mass / volume, of beads in column R amount temperature ; length / diameter, of column ; yeast concentration ; pH ; AVP ; e.g. age of culture 3 max (ii) add Benedict’s (reagent) and, boil / heat ; A CuSO4 in alkaline solution different, densities / colours (of precipitates) formed ; A turbidities use of a colorimeter in correct context ; A filtering and weighing precipitate OR use of Clinistix / Diastix (strips) ; different colours obtained ; colour compared to chart ; accept other valid methods e.g. reference to use of biosensors 2 max (iii) agree not all yeast cells successfully entrapped / AW ; (in product) yeast cells, respiring / metabolising / using sugar as an
Acid products are (with hard water, more cleaners will be no more effective than hot water, while required as minerals precipitate out with the chlorinated alkaline detergents are the most alkali cleaners), mineral deposits, undesir- effective for detaching biofilm, followed able films, and precipitates. When hard water by nonchlorinated alkaline detergents (>120 ppm CaCO3) must be used for clean- (Carpentier and Cerf 1993). Peroxides have ing, the addition of chelating or sequestrating been reported to be effective for the removal reagents is necessary.
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