2.3.2. Detection of Salivary Cortisol Salivary cortisol was dectected using a commercially available ELISA (Salimetrics, LCC, USA). Briefly, a 96-well microtitre plate coated with monoclonal antibodies to cortisol was used. Cortisol standards (3.000, 1.000, 0.333, 0.111, 0.037, and 0.012 g/ml) and the unknown compete with cortisol linked to horseradish perxidase for the antibody binding sites. Following incubation (55 mins) the plate was washed to remove unbound material. Bound cortisol peroxidase was measured by the reaction of the peroxidase enzyme on the substrate tetramethylbenzidine (TMB). After incubation for 25 mins this reaction was stopped with 50 l per well of stop solution (H 2SO4). The colour change was measured on a plate reader at 450 nm. The cortisol peroxidase detected is inversely proportional to the amount of cortisol present. 2.4 Psychosocial procedure Two rooms were used in the procedure, one room for resting and the other for Trier
Gregorio. 2002. Striated muscle cytoarchitecture: An intricate web of form and function. Annual Review and metabolic properties of skeletal muscle of Cell and Developmental Biology 18:637–706. have enormous impacts on the quality of Daun, C., M. Johansson, G. Onning, and B. Akesson. fresh meat and, in turn, its suitability as a 2001. Glutathione peroxidase activity, tissue and soluble selenium content in beef and pork in relation raw material for further processed meat. to meat ageing and pig rn phenotype. Food Chemistry Continued attention to factors that regulate 73:313–319. changes in early postmortem muscle will Diesbourg, L., H. J. Swatland, and B. M. Millman. 1988.
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