min after the TSST. The whole blood was centrifuged (250g for 10 mins) and the resulting plasma layer was removed, aliquoted and stored at 70 oC for cytokine analysis. All samples were numerically coded (known to the principal investigator) and laboratory measurements (see below) undertaken blind. 2.3.1 Detection of Plasma IL-6 Plasma IL-6 was detected using commercially available paired antibodies enabling cytokine detection in an ELISA (Enzyme Linked ImmunoAssay) format (R & D systems Ltd, Abingdon, UK). The sensitivity for the IL-6 ELISA was 9 1000 pg/ml. There was no reported cross-reactivity with other cytokines (R & D systems Ltd, Abingdon, UK). Emotion regulation in relation.. 1 2.3.2. Detection of Salivary Cortisol Salivary cortisol was dectected using a commercially available ELISA (Salimetrics, LCC, USA). Briefly, a 96-well microtitre plate coated with monoclonal antibodies to
abil paralleelselt ema verega. Kui lapse sõeluuring positiivne , siis eraldi määrata IgM kahel erineval meetodil. Nelja –või enamakordne tiitri tõus võrreldes ema tiitri väärtusega viitab kaasasündinud süüfilisele. Süüfilise seroloogilised testid: - treponemaalsed e. spetsiifilised testid: - TPHA (T. pallidum hemmagglutination assay)- IgG ja IgM koos; - TPPA (T. pallidum particle agglutation)- IgG ja IgM koos; -EIA (Ensyme immunoassay)- IgG ja IgM koos või eraldi - mittetreponemaalsed e. reagiintestid: - RPR (rapid plasma reagin); - T. pallidum immunoblot (Western blot)- eraldi IgG ja IgM . Taastuv tüüfus (vektoriga leviv haigus) HAIGUSETEKITAJA. Borrelia recurrentis. Põhjustab vahelduvate palavikuhoogudega kulgevat haigust, inimene nakatub vektori (täid, puugid) vahendusel. UURITAV MATERJAL. Paroksüsmi (kehatemperatuuri tõusu) ajal võetud veri. DIAGNOOSIMINE ALGMATERJALI MIKROSKOOPIA
annaabi.ee 
