seostub spetsiifiliselt mingi uuritava segu komponent. Kõige laialdasemalt kasutatakse kahte afiinsuskromatograafia liiki: · immuunoafiinsuskromatograafiat, · metallkelaat-afiinsuskromatograafiat. Immuunoafiinsuskromatograafia puhul on statsionaarseks faasiks kandjale seotud antigeen, mida kasutatakse antikehade eraldamiseks, näiteks, vereseerumist. Metallkelaat-afiinsuskromatograafia ehk immobiliseeritud metalliiooni afiinsuskromato- graafia (IMAC, immobilized metal ion affinity chromatography,) põhineb aminohapete tugevale interaktsioonile metalliioonidega koordinatiivsete sidemete tekkimise kaudu. Statsionaarseks faasiks on kandjale seotud kelaadi vormis metalliioonid. Enamkasutatavad on nikkel, koobalt ja vask histidiini sisaldavate valkude puhastamiseks ning raud, tsink või gallium fosforüülitud valkude ja peptiidide puhastamiseks. Reeglina on kromatografeerimisprotsessid aeglased ja labiilsete komponentide koostises võivad toimuda muutused
The amount of bound water fully characterize the role of this protein in changes very little if at all in postrigor muscle postmortem tenderization of beef. Further (Offer and Knight 1988b). studies that employ a combination of sen- Another fraction of water that can be sitive detection methods (e.g., one- and found in muscles and in meat is termed two-dimensional gels, Western blotting, entrapped (also referred to as immobilized) immunomicroscopy) are needed to determine water (Fennema 1985). The water molecules the role of filamin in skeletal muscle systems in this fraction may be held either by steric and postmortem tenderization. (space) effects and/or by attraction to the bound water. This water is held within the structure of the muscle but is not bound per
VIS Video Information System [Tandy] + Viewable Image Size + Visual Instruction Set [Sun] + Voice Information System VJ HEADER Van Jacobsen Header (compression) [Unix] VLA Variable-Length Array VLAN Virtual Local Area Network VL-BUS Vesa Local-Bus [VESA] VLD Variable-Length Decoder VLF Very Low Frequency VLINK Visited Link [HTML] VLIW Very Large Instruction Word VLM Virtual Loadable Module VLSI Very Large Scale Integration VLSIPS Very Large Scale Immobilized Polymer Synthesis VLT Variable List Table VM Virtual Machine + Virtual Memory VMA Virtual Memory Address VMB Virtual Machine Boot VMD Video Motion Detection VME Versa Module Eurocard + Virtual Memory Environment VML Vector Markup Language [Microsoft] VMM Virtual Machine/Memory Manager VMOS Vertical MOS VMP Virtual Modem Protocol VMS Virtual Machine Storage + Virtual Memory System + Voice Message System
annaabi.ee 
