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replaced with a centromere-specific variant, CENP-A in humans. 5. Peamine erinevu prokarüootse ja eukarüootse mRNA vahel. (1p) The mRNAs of many bacteria and bacteriophages are polygenic or polycistronic. A polycistronic mRNA is transcribed by the several structural genes of an operon. It contains several sites for initiating and terminating polypeptide synthesis. On the other hand all known eukaryotes have only one site for initiation of protein synthesis. Thus eukaryote mRNAs are monocistronic. In most bacterial mRNAs translation begins while the mRNA is still being transcribed on DNA. In eukaryotes the mRNA transcribed on the chromosomes passes through the nuclear pores into the cytoplasm. Here it forms complexes with ribosomes, which synthesize proteins. Thus translation usually begins only after transcription is completed. 6. Kataboliitne aktivaator (CAP-valk) seondub: (1p) c. DNA-le ÕIGE 7. Võrrelge taime- ja loomarakku
· Saab erinevaid antikehi korraga testida · Kiirdiagnostikas kasutatav · Edasiarendus: rakukiip (tulevikuteema) · kudede omadused muutuvad sügavusest ja suurendusest sõltuvalt (raku tase vs koe tase) · kallis · raskesti kvantifitseeritav · paljud antikehad parafiinlõikudel ei tööta 10. Rakukiibid new appliance to allow the screening of DNA molecules on droplets in cell chips. The aim is to cultivate series of eukaryote cells in drops, individually set on a support that will be engineered so as to carry out the screening of the DNA molecules and analyse the modifications of the transcriptome brought by the transfection. In the drops, the cell can adhere to the support or maintained in suspension kasutatakse rakkude "high-throughtput" testidena uurimaks geenifunktsioone pärast mõjutamist. Positsioonilised kiibid on tehnika kus kasutatakse võrdluseks kindlaid cDNA klastreid X,Y koordinaadistikus
or a sequence derived from a different species different traits and plant species transformed. than that of the recipient, which is called To date, cultivated GM crops mainly include sensu stricto transgene. However, both terms four plant species: soybean (53%), maize are used nowadays without distinction. GMO (30%), cotton (12%), and canola (5%). The have been developed from the three domains: most common dominant traits introduced are archaea, bacteria, and eukaryote, but only a herbicide tolerance (63%) and insect resis- very few species are commercially approved. tance (15%), with tacked double or triple In Spain, there are 45 maize varieties traits occupying a larger area (22%). approved for cultivation. There are important However, other crops are being developed to genetically modified (GM) microorganisms improve other aspects such as nutritional