kummagi ühendi ionisatsiooniefektiivsusele. Lisaks on isotopomeerid ka suhteliselt kallid ning multimeetodite korral, kui ühe kromatograafilise tsükli käigus püütakse analüüsida paljusid ühendeid korraga, ei pruugi isotooplahjendus otstarbekas olla [59]. S. Wang, M. Cyronak, E. Yang, Does a stable isotopically labeled internal standard always correct analyte response? A matrix effect study on a LC/MS/MS method for determination of carvedilol enantiomers in human plasma. J. Pharm. and Biomed. Anal. 43 (2007) 701. 4. Ekstraktsioonijärgne rikastamine. Kui prooviekstrakti rikastada analüüdiga otse enne kromatograafi süstimist, on võimalik hinnata maatriksi mõju analüüsi tulemusele. Omavahel võrreldakse kindla analüüdikontsentratsiooniga kalibreerimislahuse ning prooviekstrakti kromatograafilisi piike ja määratakse maatriksiefekt [58]. P. J. Taylor, Matrix effects: Matrix effects: The Achilles heel of quantitative high-performance
Fig. 7: A schematic representation of zone electrophoresis, isoelectric focusing and isotachophoresis Capillary zone electrophoresis (CZE) Because of their ease of use and versatile applications CZE is the most commonly used method which is primarily used for the separation of small watersoluble molecules. It is used in the analysis of amino acids, peptides, and ions of various enantiomers (optically active compounds) and many other ionic compounds. CZE it is the simplest form of HECE (High Efficiency Capillary Electrophoresis) as capillary filled only with buffer. Separation of materials into discrete zones is due to migration at different speeds. The electroosmotic flow (EOF) makes possible the separation of both cations and anions
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