Liha töötlemine
, 2003
Waxy-D1 RTi-PCR Ida et al., 2005
Commonly used techniques for plant these junctions is present in the genome of
transformation introduce a randomized and the di- or polyploid transformant, as com-
unknown copy number of the construct into mercialized GMO lines are generally hetero-
the host plant, and it may be difficult to zygous (Berdal and Holst-Jensen 2001).
accurately determine the copy number of the
integrated sequence as well as the location
Relative and Absolute Quantification
in the nuclear genome. If the detection
method targets a certain part of the gene con- Absolute quantification is the determination
struct, exact absolute quantification becomes of the amount or copy number of the target
annaabi.ee 
