"wittwer" - 1 õppematerjal

Liha töötlemine

of the target sequence (e.g., through fluores- duced in the market. cent dyes that have special fluorescent prop- Several fluorescent dsDNA-specific dyes erties when bound to double stranded (ds) have been used in RTi-PCR methods. The first DNA), and a “sequence-specific strategy” by used was ethidium bromide (Higuchi et al. sequence-specific fluorescent oligonucle- 1993; Le Pecq and Paoletti 1996; Wittwer otide probes. The latter strategy also allows et al. 1997), and subsequently, other interca- the simultaneous detection of multiple lating dyes have also been introduced, such as targets, using multiple probes labeled with YO-PRO-1 (Ishiguro et al. 1995; Tseng et al. different reporter dyes (Bernard et al. 1998; 1997) and SYBR Green I (Hernandez et al. Wittwer et al. 2001; Dupont et al. 2002). This 2003c)