Liha töötlemine
samples amenable for amplification by adap- an overestimation of yield. For this purpose
tation of extraction procedures to each food a straightforward treatment with RNAase A
matrix. The first stage is homogenization to is recommended. Then, it is simple to cor-
reduce the sample particles to an appropriate relate the amount of DNA (ng) with copy
size by grinding in homogenizers, such as number by using the C-value (Arumuganathan
blenders, stomacher, polytron, ultra-turrax, and Earle 1991; Bennett and Leitch 2003).
mills, and mortars, which reduce consider- Purity of DNA can be evaluated by assessing
ably the sampling error (Begg et al. 2007). the degree of degradation using agarose
504 Chapter 29
electrophoresis, by means of the ratio of on nested PCR are available in the bibliogra-
UV spectrophotometry at 260/280 nm (desir- phy (see Table 29.1).
ably close to 1.8) and 260/230 (above 1.8–
2
annaabi.ee 
