Liha töötlemine
Hopkins and Thompson 2002; Sentandreu et tified in mammals (Suzuki et al. 2004). Six
al. 2002). different calpains are expressed as mRNA in
Cathepsins often are not considered as the mammalian skeletal muscle, but only μ-
important in meat tenderization because their and m-calpains and p94/calpain 3 isoforms
membrane-bound location is thought to limit can be detected at the protein level (Sorimachi
substrate accessibility. Lysosomes are inca- et al. 1990; Spencer et al. 1995; Sorimachi
pable of engulfing the myofibril structure and and Suzuki 2001; Huang and Wang 2001).
no myofibrillar fragments have been identi- The Ca2+ requirements, optimum pH and
Aging/Tenderization Mechanisms 89
temperature of activity, autolysis, and inhibi- Geesink et al. 2005). Although the mRNA