"salimetrics" - 1 õppematerjal

Kvalitatiivne uurimustöö Emotsioonide regulatsioon ja stress

cytokine detection in an ELISA (Enzyme Linked ImmunoAssay) format (R & D systems Ltd, Abingdon, UK). The sensitivity for the IL-6 ELISA was 9 ­ 1000 pg/ml. There was no reported cross-reactivity with other cytokines (R & D systems Ltd, Abingdon, UK).  Emotion regulation in relation.. 1 2.3.2. Detection of Salivary Cortisol Salivary cortisol was dectected using a commercially available ELISA (Salimetrics, LCC, USA). Briefly, a 96-well microtitre plate coated with monoclonal antibodies to cortisol was used. Cortisol standards (3.000, 1.000, 0.333, 0.111, 0.037, and 0.012 g/ml) and the unknown compete with cortisol linked to horseradish perxidase for the antibody binding sites. Following incubation (55 mins) the plate was washed to remove unbound material. Bound cortisol peroxidase was measured by the reaction of the peroxidase enzyme on the substrate tetramethylbenzidine (TMB)