Liha töötlemine
This inhibition was reversible, as reaction: L-arginine+NADPH+O2 forming
addition of reducing agent (DTT) to the oxi- L-citrulline+•NO+NADPH+. NO is important
dized samples restored activity. Oxidation in biological systems, particularly because of
also has been shown to slow the rate of μ- its role as a second messenger. However,
calpain autolysis and could be part of the while NO rapidly diffuses through tissues,
mechanism underlying some of the retarda- NO itself is a relatively short-lived species.
tion of activity (Guttmann et al. 1997; Carlin It does have the ability to combine with other
et al. 2006). biomolecules that also have physiological
Oxidation does occur early in postmortem importance.
meat, and it does influence proteolysis (Harris One example of this is its ability to
et al. 2001; Rowe et al. 2004b). Rowe et al. combine with superoxide to form the highly