Liha töötlemine
radation of titin has been observed in several thin filaments (Kruger et al. 1991). Nebulin,
studies (Lusby et al. 1983; Zeece et al. 1986; via its intimate association with the thin fila-
Astier et al. 1993; Huff-Lonergan et al. 1995; ment (Lukoyanova et al. 2002), has been
Melody et al. 2004; Rowe et al. 2004a, b). hypothesized to constitute part of a compos-
When titin is degraded, a major degradation ite nebulin/thin filament (Pfuhl et al. 1994;
product, termed T2, is observed that migrates Robson et al. 1995) and may aid in anchoring
only slightly faster under SDS-PAGE con- the thin filament to the Z-line (Wang and
ditions than intact titin. This product migrates Wright 1988; Komiyama et al. 1992).
at approximately 2,400 kDa (Kurzban and Degradation of nebulin postmortem could
Wang 1988, 1987; Huff-Lonergan et al. weaken the thin filament linkages at the
1995)
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