Kvalitatiivne uurimustöö Emotsioonide regulatsioon ja stress
Abingdon, UK).
Emotion regulation in relation.. 1
2.3.2. Detection of Salivary Cortisol
Salivary cortisol was dectected using a commercially available ELISA (Salimetrics,
LCC, USA). Briefly, a 96-well microtitre plate coated with monoclonal antibodies to
cortisol was used. Cortisol standards (3.000, 1.000, 0.333, 0.111, 0.037, and 0.012
g/ml) and the unknown compete with cortisol linked to horseradish perxidase for the
antibody binding sites. Following incubation (55 mins) the plate was washed to
remove unbound material. Bound cortisol peroxidase was measured by the reaction of
the peroxidase enzyme on the substrate tetramethylbenzidine (TMB). After incubation
for 25 mins this reaction was stopped with 50 l per well of stop solution (H 2SO4).
The colour change was measured on a plate reader at 450 nm. The cortisol peroxidase
detected is inversely proportional to the amount of cortisol present.
annaabi.ee 
