Terminid
· Relative gene quantification was calculated by comparative Ct method. GAPDH expression
level was used as internal reference gene and data from three biological replicates mean ± SD
was plotted.
· Venn diagrams allow for quick visualization of relationships by
revealing intersections (overlaps) and disjunctions (non-
overlaps) for large biological datasets, and are often used in
whole-genome analysis across species. Genome wide
orthologous comparisons displayed in separate and
overlapping orthologous clusters in the Venn diagram, which
provides various shapes representing each species with
overlapping regions illustrating the genes or gene clusters that
are unique to or shared between each species. Since we were
interested only in wheat SNARE homology, we have mentioned
only those clusters containing wheat. Genome wide analysis of
orthologous clusters is an important component of
comparative genomics studies
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