Liha töötlemine
however, its role in postmortem aging is not are responsible for aging of meat. Furthermore,
fully understood (Parr et al. 1999; Ilian et al. purification of calpains and calpastatin is dif-
2000, 2001a, b, 2004; Stevenson et al. 2002; ficult to accomplish, and techniques for mea-
90 Chapter 4
suring calpain under different pH/temperature Ladrat et al. 2006). The proteasome, first
and ionic strength combinations may not isolated in 1980 (Wilk and Orlowski 1980),
provide an accurate estimation of activity is a barrel-shaped polypeptide structure with
because of precipitation of calpain or the active sites in its interior core (Mykles and
alteration of the interaction with substrates. Harie 1995; Attaix et al. 1998, 2001;
These issues have been the topic of much Glickman and Cienchanover 2002). A 20S
debate in the literature (Prates 2002; Goll et latent form is part of the 26S proteasome
al
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