Liha töötlemine
(AmpliFluor™ system) developed by Intergen baseline. During the exponential or log phase
Co. It uses a universal, energy-transfer hairpin there is an exponential increase in fluores-
primer (UniPrimer™) that emits a fluorescent cence, and finally, during the plateau phase,
signal when unfolded during its incorporation the reagents are exhausted, and no increase
into an amplification product (Nazarenko in fluorescence is observed. Quantification is
et al. 1997). only possible, therefore, at the beginning of
The “sequence-specific fluorescent the exponential phase when the reaction
probes” can be classified into two major is totally efficient and all the reagents are
groups: hydrolysis and hybridization probes. available. The most important parameter in
annaabi.ee 
