"nanomolar" - 1 õppematerjal

BIOinformaatika kodutöö 5

EISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSADLTGQANHLAATIGADIVKQKM AENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQLANCYMGRAGLINSGGAAGGETDLS DAVRTAVINKRAGGMGLILGRKAFKKSMADGVKLINAVQDVYLDSKITIA* 6) PCR Primer Stats PCR Primer Stats results Global settings: -The primers do not have a 5'-phosphate group. -Combined concentration of K+ and Na+ in the reaction = 50 millimolar. -Mg+2 concentration in the reaction = 1.5 millimolar. -Primer concentration in the reaction = 200 nanomolar. 7) Restriction Digest Restriction Digest results >326 bp linear fragment from linear parent eco:b2097 fbaB, dhnA; fructose-bisphosphate aldolase class I [EC:4.1.2.13]; K01623 fructose-bisphosphate aldolase, class I (N), base 248 to base 573 (AluI ag|ct - AluI ag|ct). cttcatttgctgctaacccgctctactttgacccgaaaaacattgttgaactggcgatcg aagcgggctgtaactgtgtggcgtcaacttacggcgtgctggcgtcggtatcgcggcgtt atgcgcatcgcattccattcctcgtcaaacttaatcacaacgagacgctaagttacccga