Liha töötlemine
concentration of the target sequence. In con- DNA detection are continuously appearing in
trast, hybridization probes are not hydrolyzed the scientific literature (Garcia-Canas et al.
during PCR. The fluorescence is generated 2002; Burns et al. 2003; Feriotto et al. 2003;
by a change in its secondary structure during Rudi et al. 2003; Glynou et al. 2004; Obeid
the hybridization phase, which results in an et al. 2004; Fantozzi et al. 2008; Morisset et
increase of the distance that separates the al. 2008). Recently, a novel DNA-based
reporter fluorophore from the quencher technology has been developed, the NASBA
moiety. The most relevant hybridization Implemented Microarray Analysis (NAIMA)
probes are those containing hairpins such as (Morisset et al. 2008). In this technology, a
Molecular Beacons, Scorpion primers, and cRNA product is obtained from a consecu-
508 Chapter 29
annaabi.ee 
