Synthesis of Ibuprofen
to higher anti-bonding molecular orbitals.
The more easily excited the electrons the longer
the wavelength of light it can absorb. Ultraviolet
(10-380 nm) or visible (380-780 nm).
HPLC
HPLC non - polar stationary phase and polar
mobile phase are used for reveres phase for
ibuprofen
Quick
Efficient
High resolution
Accurate
Analyse a blood sample
containing Ibuprofen
GC-MS can be used for the analysis the sample
Meclofenamic acid can be used as internal
standard
Sample must be dried under stream of nitrogen
Quantification
Experimental design
Alpha value 0,05;
SD 0,75;
Difference 0,25;
Power 0,08.
Using JMP program 12 was given as sample size, meaning
6 samples per instrument.
To eliminate systematic errors randomization is applied for the
samples. The experiment is expected to be completed in a day
by one person
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