"luminex" - 1 õppematerjal

Liha töötlemine

Marker Genes GMO material, as well as with commercial food samples with similar success. Fantozzi Screening methodologies exploit the detec- et al. (2008) have developed a screening pro- tion of common elements present in GMOs, tocol for the detection of the regulatory such as marker genes or regulatory sequences sequence p35S and the specific event epsps. (i.e., promoters and terminators). Most com- It is based on the Luminex xMAP technol- mercially approved GMOs have been trans- ogy, and two different sets of fluorescent formed using constructs containing sequences beads are cross-linked to the specific oligo- from the Cauliflower Mosaic Virus (CaMV, nucleotide probes previously amplified and i.e., 35S promoter [P-35S] and/or 35S termi- labeled by polymerase chain reaction (PCR) nator [T-35S]) or from Agrobacterium tume- in the presence of a biotinylated nucleotide. faciens (i