Liha töötlemine
with other changes in postmortem muscle, to as a molecular ruler that during develop-
could lead to enhanced tenderness. The deg- ment may serve to define the length of the
radation of titin has been observed in several thin filaments (Kruger et al. 1991). Nebulin,
studies (Lusby et al. 1983; Zeece et al. 1986; via its intimate association with the thin fila-
Astier et al. 1993; Huff-Lonergan et al. 1995; ment (Lukoyanova et al. 2002), has been
Melody et al. 2004; Rowe et al. 2004a, b). hypothesized to constitute part of a compos-
When titin is degraded, a major degradation ite nebulin/thin filament (Pfuhl et al. 1994;
product, termed T2, is observed that migrates Robson et al. 1995) and may aid in anchoring
only slightly faster under SDS-PAGE con- the thin filament to the Z-line (Wang and
ditions than intact titin. This product migrates Wright 1988; Komiyama et al. 1992).
annaabi.ee 
