Liha töötlemine
2000; Holck et al. 2002; (g/g%) or of weight/weight (w/w%). A criti-
Hernandez et al. 2003a; Windels et al. cal point for the relative quantification is that
2003; Nielsen et al. 2004; Taverniers et al. PCR efficiencies of each PCR system must
2004) that are unique for a single transforma- be comparable. Another analytical strategy is
tion event (event-specific). Only one copy of the use of the 2 −ΔΔCT analysis (Livak and
Assessment of Genetically Modified Organisms (GMO) in Meat Products by PCR 511
Schmittgen 2001); recently, for example, a evant issues such as the use of certified mate-
validated quantitative RTi-PCR method for rial and the validation and implementation of
MON 810 has been assessed using this useful methodologies in food-industrial pro-
approach (Aguilera et al. 2009). cesses must be carefully considered. The
annaabi.ee 
