Liha töötlemine
1995; ment (Lukoyanova et al. 2002), has been
Melody et al. 2004; Rowe et al. 2004a, b). hypothesized to constitute part of a compos-
When titin is degraded, a major degradation ite nebulin/thin filament (Pfuhl et al. 1994;
product, termed T2, is observed that migrates Robson et al. 1995) and may aid in anchoring
only slightly faster under SDS-PAGE con- the thin filament to the Z-line (Wang and
ditions than intact titin. This product migrates Wright 1988; Komiyama et al. 1992).
at approximately 2,400 kDa (Kurzban and Degradation of nebulin postmortem could
Wang 1988, 1987; Huff-Lonergan et al. weaken the thin filament linkages at the
1995). Another titin degradation product Z-line, and/or of the thin filaments in the
that has been observed by SDS-PAGE an- nearby I-band regions (Taylor et al. 1995),
alysis migrates at approximately 1,200 kDa and thereby weaken the structure of the
(Matsuura et al
annaabi.ee 
