Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
The sites of collection were not located in sequencing PCR procedures were conducted using J of the
protected or privately owned areas. recommended amounts of reagents.
To identify the species by light microscopy, spicule preparations The PCR products from five specimens were cloned into the
were made by treatment of aliquots of samples with concentrated pTZ57R/T vector using InsTAclone PCR Cloning KitTM
nitric acid for 10 min at 95uC. After removing the organic matter (Fermentas), following the manufacturer's instructions. Either
the spicules were extensively washed with water and mounted on vector specific primers or PCR product specific primers were
slides. used to sequence the cloned fragments.
Gemmules were isolated from sponge specimens and stored at
4uC in mineral medium (M medium: 0
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