Liha töötlemine
2002). exchange purification columns (Hernandez et
The DNA-based methods, especially al. 2005). One of the most commonly used
those based on its amplification, rely on dif- procedures for DNA extraction in GMO
ferent analytical steps: the DNA extraction, analysis is the CTAB-based protocol (Rogers
the DNA amplification by PCR, and finally, and Bendich 1985) or its variations (Meyer
the detection of the specific amplification and Jaccaud 1997). Binding and elution from
products. In the case of the use of real-time silica has also become the procedure of
PCR-based methods, the two later steps can choice for most nucleic acid extraction
be combined into a single one, as the ampli- procedures (Smith et al. 2003). In addition,
fication and detection occurs simultaneously many different commercial kits and auto-
in a single analytical step. mated procedures for food samples are cur-
annaabi.ee 
