"isopropanol" - 1 õppematerjal

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selection of the correct weight or volume that chelating agents (EDTA); cleanup steps must be processed from each sample. come next, using organic solvents (chloro- Generally, a portion about 0.1 to 1 g from the form, phenol); and finally, DNA is separated received sample is considered sufficient for and concentrated by alcohol/salt precipita- processing (Pietsch et al. 1997; Zimmermann tion (ethanol or isopropanol), affinity, or ion/ et al. 1998a, b, c; van Duijn et al. 2002). exchange purification columns (Hernandez et The DNA-based methods, especially al. 2005). One of the most commonly used those based on its amplification, rely on dif- procedures for DNA extraction in GMO ferent analytical steps: the DNA extraction, analysis is the CTAB-based protocol (Rogers the DNA amplification by PCR, and finally, and Bendich 1985) or its variations (Meyer