Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
In parallel,samples of sponge material were frozen in BioEdit program (http://www.mbio.ncsu.edu/bioedit/bioedit.
liquid nitrogen and stored at 220uC. html). Only sequencing data that displayed electropherograms of
Before starting, the culture gemmules were treated with 1% good quality were used.
hydrogen peroxide for 1 minute to minimize bacterial and fungal The absence or presence of notable intraindividual heteroge-
contamination [8]. Sponges were hatched on Petri dishes in M neity was assessed by visual examination of the electropherograms
medium. Fully functional young sponges were removed from the obtained by direct sequencing. The presence of heterogeneities
dishes using a scraper and dispersed in calcium and magnesium was indicated by double peaks in substitution positions, and by a
free medium (CMF medium: 0.1 mM NaHCO3, 0
annaabi.ee 
