RNAsid on erinevaid: mRNA – informatsiooni RNA (messenger RNA). Peamine RNA molekul, mis transleeritakse valguks. tRNA – transpordi RNA (transfer RNA). Madalmolekulaarsed RNA-d, mis on vajalikud translatsiooni läbi viimiseks. Toimivad adapteritena mRNA koodonite ja aminohapete vahel. rRNA – ribosoomi RNA (ribosomal RNA). Ribosoomi struktuuri osad, ka funktsionaalse tähtsusega. hnRNA – heterogeenne tuuma RNA (heterogenous nuclear RNA) on esmane (primaarne) transkript ja sisaldab introneid ega ole kodeeriv. Protsessitakse mRNA-ks tsütoplasmas. snRNA - väike tuuma RNA (small nuclear RNA). Osalevad splaissosoomide töös. Toimib tuumas. miRNA – mikro RNA (microRNA). Väikesed, 20-25 nt. Reguleerivad geenide avaldumist. Transkriptsiooni viib läbi RNA polümeraas RNA polümeraas liigub DNA molekulil 5’-˃3’ suunas, harutades samal ajal lahti DNA molekuli.
direct sequencing data were comparable to the amounts of clones had 253 nt, but 2 clones had the shortest ITS1 variant, sequence variants from 12 clones of the same specimen V2. When Figure 3. Schematic representation of heterogeneities found in ITS1 and ITS2 revealed by cloning from 5 specimens. Red and blue rectangles mark the locations of indels and substitutions, respectively. The heterogenous positions (accompanied by a few surrounding nucleotides) found in those locations are given in the boxes below. doi:10.1371/journal.pone.0066601.g003 PLOS ONE | www.plosone.org 5 June 2013 | Volume 8 | Issue 6 | e66601 Intragenomic Profiling Using Multicopy Genes Table 1