Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
Then to
amplified using the primers 18Sfw: 59-TAC ACA CCG CCC obtain the expected values, the mean value among clones for a
GTC GCT ACT A and 28S59rev: 59-GAC GTG CCT TTC particular nucleotide in each position was found. The normaliza-
CAG GTC AAC TT. The primers were designed to include the tion was needed also because different primers were used for
invariant sequence before the heterogenic positions in the sequencing the clones, so any particular nucleotide in the rDNA
amplicon. This allowed the assessment of the quality of the was positioned at a different distance from the primer.
sequencing electropherogram data obtained. The data obtained by direct sequencing were normalized in a
annaabi.ee 
