Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
for 18S, 5.8S, and 28S rRNAs. Between these genes, on either side adequately analyse the heterogeneity of the gene pool. We hereby
of the 5.8S rRNA gene, the internal transcribed sequences (ITS), describe a method that also allows for the quantification of the
ITS1 and ITS2, are located [1]. In order to preserve the amounts of different alleles containing heterogeneities caused by
functionality of multicopy genes, concerted evolution is at play. indel events. We use the ITS sequences of the freshwater sponge
However, homogenisation of all the gene variants is not always Ephydatia fluviatilis as an example, and describe intra-individual
complete [2]. Divergent copies may interfere in obtaining heterogeneity in this species for the first time. Our method enables
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