Kvalitatiivne uurimustöö Emotsioonide regulatsioon ja stress
their emotions. Higher scores mean higher HER, stronger buffering of emotions in
EM, and higher distress augmenting regulation in DAR.
2.3 Physiological stress response measures.
Participants collected their saliva by chewing plain (non-citric acid) cotton Salivettes
and the samples (8 time points as described in section 2.4 on p 15) were frozen at
20oC prior to assaying for cortisol. Peripheral venous blood (2 ml anticoagulant with
heparin) was obtained by a trained phlebotomist 10 min before the TSST and again 50
min after the TSST. The whole blood was centrifuged (250g for 10 mins) and the
resulting plasma layer was removed, aliquoted and stored at 70 oC for cytokine
analysis. All samples were numerically coded (known to the principal investigator)
and laboratory measurements (see below) undertaken blind.
2.3.1 Detection of Plasma IL-6
Plasma IL-6 was detected using commercially available paired antibodies enabling
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