Personal relationships and danna Vocabulary Geisha Maiko Okiya Ozashiki Onee-san Minarai Becoming a Geisha Apprenticeship: Strict Learning traditional arts Minarai Onee-san Ozashiki sit and observe ->potential clients Erikae White collar Hairstyle changes Maiko Minarai Appearance Makeup Most recognisable characteristics White base, red lipstick, red or black eyes and eyebrows Dress: kimono with obi and zoris and tabis Hair Hair-combs and hairpins No pillow wig zoris tabis Click to edit Master text styles Second level Third level Fourth level Fifth level A geisha, when she is working, is just that: the illusion of female perfection. Geisha and prostitution Geisha is not a prostitute! "Geisha girls" Served Am soldiers General word for any Japanese prostitute
by a change in its secondary structure during Rudi et al. 2003; Glynou et al. 2004; Obeid the hybridization phase, which results in an et al. 2004; Fantozzi et al. 2008; Morisset et increase of the distance that separates the al. 2008). Recently, a novel DNA-based reporter fluorophore from the quencher technology has been developed, the NASBA moiety. The most relevant hybridization Implemented Microarray Analysis (NAIMA) probes are those containing hairpins such as (Morisset et al. 2008). In this technology, a Molecular Beacons, Scorpion primers, and cRNA product is obtained from a consecu- 508 Chapter 29 tive first primer extension reaction, using junction regions of DNA from different tailed primers, and a subsequent transcrip- origins, and flanking regions of the intro- tion-based amplification, using universal duced construction, which aims to determine primers
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