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"hahnen" - 1 õppematerjal

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in a single analytical step. mated procedures for food samples are cur- rently available (Marmiroli et al. 2008), although the automation is restricted nowa- DNA Extraction from Food Matrices days to a limited number of food samples The first step of PCR-based methods in (Hahnen et al. 2002). Several papers compare food analysis relies on a careful DNA- different extraction methods (Zimmermann extraction procedure, since components of et al. 1998a; Peano et al. 2004). food samples and nucleic acid extraction After DNA extraction, quantity and reagents can reduce or even block the PCR quality must be evaluated by means of UV amplification, and DNA may be degraded. spectrophotometry at 260 nm; by fluorimetry

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