"genomed" - 1 õppematerjal

Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs

Ko~pu (specimens K1 and K2). The study was approved by The electrophoresis of the products in 1% agarose gel. The amplifi- Estonian Environmental Board. No additional permits or approv- cation products were purified using the JetQuick PCR Purification als were required. The cosmopolitan sponge species Ephydatia Kit (Genomed, Germany). fluviatilis is a representative of the phylogenetically lowest DNA fragments of ,1091 bp obtained by PCR amplification multicellular animals and there are no ethics regulations concern- were directly sequenced using ABI PRISM 3130 Genetic Analyzer ing this species. E. fluviatilis is widely distributed in Estonia and not (Applied Biosystems) with the primers mentioned above. All under protection