Liha töötlemine
of carbonyl protein groups with the 2,4- ing the development of increased bacterial
dinitrophenylhydrazine (DNPH) to form resistance to certain antibiotics due to the
hydrazones, and then the absorbance is mea- abuse of antibiotics consumption (Butaye
sured at 370 nm (Oliver et al. 1987). Another et al. 2001).
method to evaluate protein oxidation is based Most veterinary drugs have been banned
on the conjugated fluorophores resulting in the European Union for use in farm animals
from reactions between lipid oxidation prod- because of fears about health effects (geno-
ucts (aldehydes) and amino groups. This toxic, immunotoxic, carcinogenic, or endo-
fluorescence can be detected at excitation and crine) from their residues in animal tissues.
emission wavelengths of 350 and 450 nm, These substances can only be administered to
respectively (Viljanen et al. 2004)