Molekulaardiagnostika kordamisküsimused
4. SNP genotyping.
5. Verification of microarray results.
Molecular Beacons:
Structure:
A molecular beacon consists of 4 parts, namely
-Loop: This is the 18-30 base pair region of the molecular beacon which is
complementary to the target sequence.
-Stem: The beacon stem sequence lies on both the ends of the loop. It is typically 5-7 bp
long at the sequences at both the ends are complementary to each other.
-5' fluorophore: Towards the 3' end of the molecular beacon, is attached a dye that
fluoresces in presence of a complementary target.
-3' quencher (non fluorescent): The quencher dye is covalently attached to the 3' end of
the molecular beacon and when the beacon is in closed loop shape, prevents the
fluorophore from emitting light.
Molecular Beacons Functioning:
Molecular beacons can report the presence of specific nucleic acids from a homogeneous
solution. In the presence of a complementary target, the "stem" portion of the beacon
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