Liha töötlemine
Other DNA Techniques
1991), yielding a real-time, measurable fluo-
rescence emission directly proportional to the Other modifications and techniques based on
concentration of the target sequence. In con- DNA detection are continuously appearing in
trast, hybridization probes are not hydrolyzed the scientific literature (Garcia-Canas et al.
during PCR. The fluorescence is generated 2002; Burns et al. 2003; Feriotto et al. 2003;
by a change in its secondary structure during Rudi et al. 2003; Glynou et al. 2004; Obeid
the hybridization phase, which results in an et al. 2004; Fantozzi et al. 2008; Morisset et
increase of the distance that separates the al. 2008). Recently, a novel DNA-based
reporter fluorophore from the quencher technology has been developed, the NASBA
moiety. The most relevant hybridization Implemented Microarray Analysis (NAIMA)
annaabi.ee 
