62 Molekulaarsed meetodid mikroorganismide samastamiseks ja iseloomustamiseks 9 Eelnevad peatükid on käsitlenud bakterite, seente ja algloomade identifikatsiooni ja iseloomustamist, lähtudes fenotüübilistest meetoditest. Fenotüübil põhinevatel meetoditel on mitmeid puudusi: • Teatud nõudlikud bakterid (fastidious pathogenes) ei ole suutelised kasvama söötmetel; • Mõned mikroorganismid ei säili eluvõimelistena aja vältel, mis kulub uuritava materjali laborisse jõudmiseks; • Mikroorganismide kasv kunstlikel söötmetel võib nõuda väga pikka aega (nädalad, kuud), nende samastamine võib nõuda väga suuri materiaalseid ressursse; • Puuduvad meetodid mõnede mikroobide samastamiseks fenotüübiliste tunnuste alusel.
minutes, to press his friend to join it. "Come, Darcy," said he, "I must have you dance. I hate to see you standing about by yourself in this stupid manner. You had much better dance." "I certainly shall not. You know how I detest it, unless I am particularly acquainted with my partner. At such an assembly as this it would be insupportable. Your sisters are engaged, and there is not another woman in the room whom it would not be a punishment to me to stand up with." "I would not be so fastidious as you are," cried Mr. Bingley, "for a kingdom! Upon my honour, I never met with so many pleasant girls in my life as I have this evening; and there are several of them you see uncommonly pretty." "You are dancing with the only handsome girl in the room," said Mr. Darcy, looking at the eldest Miss Bennet. "Oh! She is the most beautiful creature I ever beheld! But there is one of her sisters sitting down just behind you, who is very pretty, and I dare say very agreeable. Do let me
individual manner the modern town dwellers and their environment. The Fourth Symphony stands out as something different.1 Manifold, even kaleidoscopic materials and jazz elements are used. The pulse in the fast movements is an axis on which all incessant modifications are based. A Trombone motif constitutes the foundation for the first movement that resembles a block built up of motifs: Example 86. The themes could be more extensive. The author seems not to be fastidious enough. The second movement (Fl solo) sounds calm and epic. 1 First performance: 25 Dec. 1959. ERSO conducted by Roman Matsov. The following two movements contain neither outstanding thematic materials nor the character of fixed subjects. A row of single contemplating motifs appears in the fourth movement. An example: Example 87. The Finale includes one theme with a core consisting of only one bar. The general feeling is one of lamentation and nervousness
a house of cards collapsing. All business relationships are based on trust. All relationships that involve money are dependent on the word of the borrower or the creditor. All relationships with your bankers, your suppliers, your customers, your staff, and everyone else in your financial world are based on that critical element of trust. Men and women of high integrity are fastidious about the levels of trust that they have built and maintained. They are careful about their credit, and about their financial commitments and arrange- ments. They always keep their word. They are careful about their banking relationships, their credit cards, their bills, and any money that they owe at any time. ccc_tracy_12_222-248.qxd 6/23/03 3:39 PM Page 233
annaabi.ee 
