Liha töötlemine
Reference Genes
two introduced regions from different origins
In a food sample, only a certain percentage (Kuribara et al. 2002; Hernandez et al.
may contain the GM ingredient. In order to 2004a); and event-specific methods, which
identify the total amount of DNA corre- amplify the border region between the intro-
sponding to that particular GMO, endoge- duced sequence and the plant genomic DNA
nous reference controls that define 100% of (e.g., for Roundup Ready soybean: Berdal
each ingredient must be used. These are and Holst-Jensen 2001; Taverniers et al.
based on genes present in both transgenic and 2001; Terry and Harris 2001), for transgenic
nontransgenic varieties of the same plant maize lines, such as MON810 (Holck et al.
species